Results 1 to 10 of about 2,171,089 (338)

RT-PCR negative COVID-19 [PDF]

BMC Infectious Diseases, 2022
Background COVID-19 is a multi-system infection with emerging evidence-based antiviral and anti-inflammatory therapies to improve disease prognosis. However, a subset of patients with COVID-19 signs and symptoms have repeatedly negative RT-PCR tests ...
Heta Parmar, Margaret Montovano, Padmapriya Banada, Sri Ram Pentakota, Stephanie Shiau, Zhongjie Ma, Kaheerman Saibire, Abby Chopoorian, Michael O’Shaughnessy, Mitchell Hirsch, Priyanshi Jain, Gaiane Demirdjian, Magali Karagueuzian, Thomas Robin, Michael Salvati, Bhavana Patel, David Alland, Yingda L. Xie   +17 more
doaj   +3 more sources

Comparison of Three RT-PCR Methods [PDF]

BioTechniques, 1998
Various approaches can now be taken for amplification of RNA transcripts using the polymerase chain reaction (PCR). Here, we compare three such methods: (i) uncoupled reverse transcription (RT)-PCR (using separate reactions for cDNA synthesis and PCR ...
Loryn N. Sellner, Gavin R. Turbett
doaj   +3 more sources

Quantitative RT-PCR: Limits and Accuracy

BioTechniques, 1996
In this paper we determine the limits and accuracy of quantitative reverse transcription (RT)-PCR using a modification of the original protocol. The quantification of mRNA with this procedure requires a preliminary estimation of the target molecule (TM ...
F. Souazé, A. Ntodou-Thomé, C.Y. Tran, W. Rostène, P. Forgez   +4 more
doaj   +5 more sources

Evaluation of the Effects of DNase Treatment on Signal Specificity in RT-PCR and In Situ RT-PCR [PDF]

BioTechniques, 1998
In this study, the effects of DNase treatment on the specificity of reverse transcription (RT)-PCR have been investigated on different samples, containing RNA, DNA, DNA/RNA and DNA/cDNA.
Karin Ivarsson, Birgitta Weijdegård
doaj   +3 more sources

RT-PCR Without RNA Isolation

BioTechniques, 1996
Reverse transcription-PCR (RT-PCR) has traditionally required time-consuming RNA extraction and purification. This report demonstrates that one can completely avoid the RNA extraction step in RT-PCR by basing the comparison of samples on cell number ...
R.J. Klebe, G.M. Grant, A.M. Grant, M.A. Garcia, T.A. Giambernardi, G.P. Taylor   +5 more
doaj   +4 more sources

Quantitative RT-PCR: Pitfalls and Potential

BioTechniques, 1999
Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing RNA. While it has tremendous potential for quantitative applications, a comprehensive knowledge of its technical aspects is required. Successful quantitative RT-PCR
Willard M. Freeman, Stephen J. Walker, Kent E. Vrana   +2 more
doaj   +4 more sources

RT-PCR with affinity-captured mRNA [PDF]

Nucleic Acids Research, 1993
Kevin Cuddy, Brian Foley, Jonathan S. Jaffe, David Gillespie   +3 more
openalex   +5 more sources

Pitfalls of Processed Pseudogenes in RT-PCR [PDF]

BioTechniques, 1998
Helen Mutimer, Nicholas Deacon, Suzanne Crowe, Secondo Sonza   +3 more
doaj   +3 more sources

Exploring Demographic Properties and Amplification Efficiency of N and ORF-1ab Gene in Suspected COVID-19 Patients in Dhaka, Bangladesh

Update Dental College Journal, 2022
Background: In late December 2019, an outbreak of pneumonia with an unknown cause occurs in Wuhan, China. Later, the causative agent was identified as SARS-CoV-2.
Sumaiya khatun , Md Moazzem Hossain , Md Zaforullah Chowdhury, Mamun Mia, Nazmun Nahar Munny, Fatima Tuj Johora   +5 more
doaj   +3 more sources

RT-PCR for multi-copy genes

BioTechniques, 2013
Kristie Nybo
doaj   +2 more sources