Results 1 to 10 of about 1,155,913 (246)

RT-PCR negative COVID-19 [PDF]

BMC Infectious Diseases, 2022
Abstract Background COVID-19 is a multi-system infection with emerging evidence-based antiviral and anti-inflammatory therapies to improve disease prognosis. However, a subset of patients with COVID-19 signs and symptoms have repeatedly negative RT-PCR tests, leading to treatment hesitancy.
Heta Parmar, Margaret Montovano, Padmapriya Banada, Sri Ram Pentakota, Stephanie Shiau, Zhongjie Ma, Kaheerman Saibire, Abby Chopoorian, Michael O’Shaughnessy, Mitchell Hirsch, Priyanshi Jain, Gaiane Demirdjian, Magali Karagueuzian, Thomas Robin, Michael Salvati, Bhavana Patel, David Alland, Yingda L. Xie   +17 more
openaire   +3 more sources

Direct In Situ RT-PCR [PDF]

, 2011
In situ polymerase chain reaction (PCR) is a histological technique that exploits the advantages of PCR for detection of mRNA directly in tissue sections. It somehow conjugates together PCR and in situ hybridization that is more traditionally employed for mRNA localization in cell organelles, intact cells, or tissue sections. This chapter describes the
LOSSI, Laura, GAMBINO, Graziana, SALIO, Chiara, MERIGHI, Adalberto   +3 more
openaire   +4 more sources

Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples [PDF]

, 2005
Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for
Al Rashid S.T., Cloak O.M., Hani E.C., Hunt J.M., Linton D., Manser P.A., Munroe D., Ng L.-K., Penner J.L., Totten P.A., Wilson D.L.   +10 more
core   +1 more source

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]

, 2019
Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A., Caruso A. G., Davino S., Panno S., Ruiz-Ruiz S., San Ambrosio M. I. F.   +5 more
core   +1 more source

RT‐PCR in foot‐and‐mouth

Veterinary Quarterly, 1998
A RT-PCR assay for the specific detection of RNA sequences from foot-and-mouth disease virus (FMDV) has been developed. The procedure permits also the detection of sequences that correlate with established FMDV serotypes. A computer program that allows selection of genotype-specific primers for RT-PCR amplification was used for the identification of ...
Dopazo, J., Núñez, J. I., Blanco Lavilla, Esther, Hernández, T., Sobrino Castelló, Francisco, Núñez, J. I., Hernández, T., Sobrino Castelló, Francisco   +7 more
openaire   +4 more sources

Molecular detection and phylogenetic analysis of Peste des petits ruminants virus circulating in small ruminants in eastern Amhara region, Ethiopia [PDF]

, 2019
Background: Peste des Petits Ruminants (PPR) is a severe, highly infectious and fatal viral disease of small ruminants. Four lineages of PPR virus have been identified globally based on sequence analysis of the nucleoprotein (N) and fusion (F) gene.
Abdi, Reta D., Alemu, Biruk, Asfaw, Wondwoson, Belayneh, Rediet, Gari, Getachew, Kidane, Menbere, Kwiatek, Olivier, Libeau, Geneviève, Siraw, Bewuket, Wieland, Barbara   +9 more
core   +1 more source

Multiplex Detection of Aspergillus fumigatus Mycoviruses. [PDF]

, 2018
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Mycoviruses are ...
Latge, Robert Coutts, Selin Özkan-Kotiloğlu   +2 more
core   +2 more sources

Prevalence of inclusion body disease and associated comorbidity in captive collections of boid and pythonid snakes in Belgium [PDF]

, 2020
Inclusion body disease (IBD) is caused by reptarenaviruses and constitutes one of the most notorious viral diseases in snakes. Although central nervous system disease and various other clinical signs have been attributed to IBD in boid and pythonid ...
Hellebuyck, Tom, Leineweber, Christoph, Marschang, Rachel E, Simard, Jules   +3 more
core   +1 more source

Quantitative PCR and RT-PCR in virology. [PDF]

Genome Research, 1993
In the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in ...
CLEMENTI M, MENZO S, BAGNARELLI P, MANZIN, ALDO, VALENZA A, VARALDO PE   +5 more
openaire   +4 more sources

Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples [PDF]

, 2014
Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more
Reena Ghildyal, Xuelian Yu, Xuelian Yu
openaire   +3 more sources