Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples [PDF]
, 2005 Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for
Al Rashid S.T. +10 more
core +1 more source
Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]
, 2019 Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A. +5 more
core +1 more source
Analysis of the bacterial communities in the rhizosphere of Chrysanthemum via denaturing gradient gel electrophoresis of PCR- amplified 16S rRNA as well as DNA fragments coding for 16S rRNA [PDF]
, 2001 The effect of developing chrysanthemum roots on the presence and activity of bacterial populations in the rhizosphere was examined by using culture-independent methods.
Duineveld, B.M. +4 more
core +1 more source
Use of reverse transcription-polymerase chain reaction (RT-PCR) for Cymbidium mosaic virus (CyMV) detection in orchids [PDF]
, 2000 The reverse transcription-polymerase chain reaction CRT-PCR) was adapted for detection of Cymbidium mosaic virus CCyMV) in orchids. The oligonucleotide primers used were selected from the predicted homologous coat protein region of CyMV and other ...
Benjamin Hescott +3 more
core +7 more sources
Molecular detection and phylogenetic analysis of Peste des petits ruminants virus circulating in small ruminants in eastern Amhara region, Ethiopia [PDF]
, 2019 Background: Peste des Petits Ruminants (PPR) is a severe, highly infectious and fatal viral disease of small ruminants. Four lineages of PPR virus have been identified globally based on sequence analysis of the nucleoprotein (N) and fusion (F) gene.
Abdi, Reta D. +9 more
core +1 more source
Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]
, 2018 Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra +5 more
core +1 more source
Multiplex Detection of Aspergillus fumigatus Mycoviruses. [PDF]
, 2018 © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Mycoviruses are ...
Latge +2 more
core +3 more sources
Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]
Archives of Biological Sciences, 2012 Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D. +6 more
doaj +1 more source
Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]
, 2009 Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E. +3 more
core +2 more sources
Molekularbiologische Diagnostik von Pflanzenkrankkeiten
Julius-Kühn-Archiv, 2014 In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine +3 more
doaj +1 more source