RT-PCR Analysis of TOPBP1 Gene Expression in Hereditary Breast Cancer [PDF]
, 2010 Hereditary predisposition to breast cancer determined in large part by loss of function mutations in one of two genes BRCA1 and BRCA2. Besides BRCA1 and BRCA2 other genes are also likely to be involved in hereditary predisposition to breast cancer ...
Bernaciak, Magdalena +3 more
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Characterisation of nicotine receptors on human peripheral blood mononuclear cells (PBMC) [PDF]
, 2009 “The original publication is available at www.springerlink.com”. Copyright Springer. DOI: 10.1007/s00011-008-8171-xAim and objective: The aim of the work was to characterise the nAChRs on human PBMC.
Parsons, M. +2 more
core +1 more source
Quantitative RT-PCR: Limits and Accuracy
BioTechniques, 1996 In this paper we determine the limits and accuracy of quantitative reverse transcription (RT)-PCR using a modification of the original protocol. The quantification of mRNA with this procedure requires a preliminary estimation of the target molecule (TM) concentration, established from experiments with an internal control molecule (ICM).
Souazé, Frédérique +4 more
openaire +6 more sources
Evaluation of a commercially available reverse transcription-PCR enzyme immunoassay (Enterovirus Consensus kit) for the diagnosis of enterovirus central nervous system infections [PDF]
, 2005 A commercial reverse transcription (RT)-PCR amplification method was compared with culture for the diagnosis of enterovirus meningitis. In total, 99 cerebrospinal fluid (CSF) specimens were examined with the Enterovirus Consensus kit and shell vial ...
Gartzonika, C. +2 more
core +1 more source
Use of reverse transcription-polymerase chain reaction (RT-PCR) for Cymbidium mosaic virus (CyMV) detection in orchids [PDF]
, 2000 The reverse transcription-polymerase chain reaction CRT-PCR) was adapted for detection of Cymbidium mosaic virus CCyMV) in orchids. The oligonucleotide primers used were selected from the predicted homologous coat protein region of CyMV and other ...
Benjamin Hescott +3 more
core +7 more sources
The Appropriate Use of Testing for COVID-19 [PDF]
, 2020 Many public officials are calling for increased testing for the 2019 novel coronavirus disease (COVID-19), and some governments have taken extraordinary measures to increase the availability of testing.
Zitek, Tony
core
An autocrine role for pituitary GABA: Activation of GABA-B receptors and regulation of growth hormone levels [PDF]
, 2002 There is increasing evidence suggesting that the neurotransmitter gamma-aminobutyric acid (GABA) is a local factor involved in the regulation of endocrine organs.
Corsi, C. +5 more
core +1 more source
Detection and identification of Fabavirus species by one-step RT-PCR and multiplex RT-PCR
Journal of Virological Methods, 2014 The genus Fabavirus of the family Secoviridae comprises a group of poorly characterized viruses. To date, only five species have been described: Broad bean wilt virus 1 (BBWV-1), Broad bean wilt virus 2 (BBWV-2), Lamium mild mosaic virus (LMMV), Gentian mosaic virus (GeMV) and Cucurbit mild mosaic virus (CuMMV).
Panno, S +7 more
openaire +5 more sources
, 2018 This assay amplified the partial VP1 gene (Region C) of the norovirus genome. The yield products could be sequenced.
Hengyun Guan +3 more
openaire +2 more sources
Modeling and analysis of competitive RT-PCR [PDF]
Nucleic Acids Research, 1998 The present studies demonstrate a theoretical and practical framework for the accurate quantitation of gene expression in RNA extracted from microscopic tissue samples. The approaches are developed around competitive RT-PCR techniques. Assay performance has been examined and validated at both the RT and PCR steps.
Peter J. Oefner +5 more
openaire +3 more sources