Results 21 to 30 of about 1,247,639 (287)
, 2011 In situ polymerase chain reaction (PCR) is a histological technique that exploits the advantages of PCR for detection of mRNA directly in tissue sections. It somehow conjugates together PCR and in situ hybridization that is more traditionally employed for mRNA localization in cell organelles, intact cells, or tissue sections. This chapter describes the
LOSSI, Laura +3 more
openaire +3 more sources
The Appropriate Use of Testing for COVID-19 [PDF]
, 2020 Many public officials are calling for increased testing for the 2019 novel coronavirus disease (COVID-19), and some governments have taken extraordinary measures to increase the availability of testing.
Zitek, Tony
core
Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR [PDF]
, 2017 Background Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses ...
Agoti, Charles N. +8 more
core +2 more sources
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2006 Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV)
A. Cortez +8 more
doaj +1 more source
Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques [PDF]
, 2001 Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment.
Antunes, S.v. +5 more
core +1 more source
RT-PCR Analysis of TOPBP1 Gene Expression in Hereditary Breast Cancer [PDF]
, 2010 Hereditary predisposition to breast cancer determined in large part by loss of function mutations in one of two genes BRCA1 and BRCA2. Besides BRCA1 and BRCA2 other genes are also likely to be involved in hereditary predisposition to breast cancer ...
Bernaciak, Magdalena +3 more
core +2 more sources
Evaluation of the Effects of DNase Treatment on Signal Specificity in RT-PCR and In Situ RT-PCR
BioTechniques, 1998 In this study, the effects of DNase treatment on the specificity of reverse transcription (RT)-PCR have been investigated on different samples, containing RNA, DNA, DNA/RNA and DNA/cDNA.
Karin Ivarsson, Birgitta Weijdegård
doaj +1 more source
Brazilian Journal of Infectious Diseases, 2021 Introduction: In the current standard of care (SoC) RT-PCR method for COVID-19, the patient's swab was extracted in viral transport media (VTM). For the Panbio™ COVID-19 Ag Rapid Test, the patient swab is flushed out in extraction buffer, of which a ...
Terezinha Marta Pereira Pinto Castineiras +8 more
doaj +1 more source
Characterisation of nicotine receptors on human peripheral blood mononuclear cells (PBMC) [PDF]
, 2009 “The original publication is available at www.springerlink.com”. Copyright Springer. DOI: 10.1007/s00011-008-8171-xAim and objective: The aim of the work was to characterise the nAChRs on human PBMC.
Parsons, M. +2 more
core +1 more source
BREAST CANCER TYPING USING RT-PCR ASSAY
Сибирский онкологический журнал, 2019 Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy.
V. K. Bozhenko +6 more
doaj +1 more source