Results 21 to 30 of about 2,171,089 (338)

In Situ RT-PCR Using Fluorescence-Labeled Primers

BioTechniques, 1997
Ulrike Stein, Wolfgang Walther, Jana Wendt, Thomas A. Schild   +3 more
doaj   +2 more sources

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Genome Biology, 2002
BackgroundGene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes.
J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, F. Speleman   +6 more
semanticscholar   +1 more source

Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2020
Severe acute respiratory syndrome coronavirus 2 viral load in the upper respiratory tract peaks around symptom onset and infectious virus persists for 10 days in mild-to-moderate coronavirus disease (n = 324 samples analysed). RT-PCR cycle threshold (Ct)
A. Singanayagam, M. Patel, A. Charlett, J. Lopez Bernal, V. Saliba, J. Ellis, S. Ladhani, M. Zambon, R. Gopal   +8 more
semanticscholar   +1 more source

Comparison of Home Antigen Testing With RT-PCR and Viral Culture During the Course of SARS-CoV-2 Infection.

JAMA Internal Medicine, 2022
Importance As self-collected home antigen tests become widely available, a better understanding of their performance during the course of SARS-CoV-2 infection is needed.
V. Chu, N. Schwartz, Marisa A P Donnelly, Meagan R. Chuey, R. Soto, A. Yousaf, Emily N. Schmitt-Matzen, S. Sleweon, Jasmine Ruffin, N. Thornburg, J. Harcourt, A. Tamin, Gimin Kim, J. Folster, Laura J. Hughes, S. Tong, Ginger Stringer, B. Albanese, S. Totten, Meghan M. Hudziec, Shannon R. Matzinger, E. Dietrich, Sarah W. Sheldon, Sarah Stous, E. McDonald, B. Austin, M. Beatty, J. E. Staples, M. Killerby, Christopher H. Hsu, J. Tate, H. Kirking, Almea M Matanock, W. Abara, L. Alexander, Melissa M. Arons, M. Firestone, M. Foster, E. Garza, Yessica Gomez, S. Guagliardo, D. Haberling, B. Humrighouse, Stacey L. Konkle, Perrine Marcenac, Benjamin P. Monroe, Apophia Namageyo-Funa, M. O’Hegarty, C. Pratt, Ian W. Pray, Byron F. Robinson, H. Ruiseñor-Escudero, H. Segaloff, Talya Shragai, Tarah Somers, F. Tobolowsky, R. Zacks, Raydel D. Anderson, A. Chakrabarti, Blake Cherney, Stephen P. LaVoie, Kaitlin F. Mitchell, Clint N. Morgan, R. Rossetti, R. Stoddard, Jeni T. Vuong, M. Whaley, J. Castro Georgi, R. Chancey, Erica Figueroa, J. Lehman, Kristine Lindell, Reed Magleby, G. Marx, D. McCormick, Paul Mead, Laird J. Ruth, Maggie Silver, S. Visser, M. Waltenburg, Karen L. Boroughs, A. Brault, A. Drexler, Janet McAllister, Jamie L. Pawloski, Janae L. Stovall, Maria I. Dionicio Bernabe, A. L. Manlutac, Graciela Zuniga-Groot, Seema Jain, H. Baily, Alexis W. Burakoff, Christopher Grano, L. Webb, Evelyn Álvarez, Cassandra N Bundalian, Hannah Collins, Christopher Delmonico, Shaun Dunyak, Hannah M Fortune, Wyatt Giddmore, Kimberly Huynh-Templeman, T. Ling, Hunter Olsen, Nicholas J Pysnack, Aleigha M Ricci, Tara M. Stitzlein, J. Drobeniuc, Sabrina Weis-Torres, L. Punkova, T. Jia, Peter Browning, Shanna J Bolcen, P. Maniatis, So Hee Park, K. M. Moss, B. Zellner, K. Ortiz, A. Uehara, Adam C. Retchless, Ying Tao, Yan Li, Anna Kelleher, H. Ng, Jing Zhang, Brian Lynch, P. W. Cook, Clinton R. Paden, Krista J. Queen, M. Medrzycki, Shannon L. Rogers, Phili Wong, Shilpi Jain, A. Tejada-Strop, J. Metz, E. David, Xiaoling Tang, N. Wynn, R. Tiller, S. Sakthivel, Srinivasan Velusamy, Brett Whitaker, Marla Petway   +142 more
semanticscholar   +1 more source

A new mathematical model for relative quantification in real-time RT-PCR.

Nucleic Acids Research, 2001
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression.
M. Pfaffl
semanticscholar   +1 more source

Real-time RT-PCR in COVID-19 detection: issues affecting the results

Expert Review of Molecular Diagnostics, 2020
Due to the rapid spread and increasing number of coronavirus disease 19 (COVID-19) cases caused by a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), rapid and accurat...
A. Tahamtan, A. Ardebili
semanticscholar   +1 more source

Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA

BioTechniques, 1998
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps.
L.L. Hall, G.R. Bicknell, L. Primrose, J.H. Pringle, J.A. Shaw, P.N. Furness   +5 more
doaj   +1 more source

Stability issues of RT‐PCR testing of SARS‐CoV‐2 for hospitalized patients clinically diagnosed with COVID‐19

Journal of Medical Virology, 2020
In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR) testing ...
Yafang Li, L. Yao, Jiawei Li, Lei Chen, Yiyan Song, Z. Cai, Chunhua Yang   +6 more
semanticscholar   +1 more source

Analysis of the initial lot of the CDC 2019-Novel Coronavirus (2019-nCoV) real-time RT-PCR diagnostic panel

PLoS ONE, 2021
At the start of the COVID-19 pandemic, the Centers for Disease Control and Prevention (CDC) designed, manufactured, and distributed the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel for SARS-CoV-2 detection.
Justin Lee, J. Goldstein, J. L. Moon, O. Herzegh, D. Bagarozzi, M. Oberste, H. Hughes, K. Bedi, Dorothie Gerard, Brenique Cameron, Christopher Benton, A. Chida, Ausaf Ahmad, David Petway, Xiaoling Tang, Nicky Sulaiman, Dawit Teklu, D. Batra, Dakota T. Howard, M. Sheth, W. Kuhnert, S. Bialek, C. Hutson, J. Pohl, Darin S. Carroll   +24 more
semanticscholar   +1 more source

False-negative results of initial RT-PCR assays for COVID-19: A systematic review

medRxiv, 2020
Background: Cases with negative reverse transcription-polymerase chain reaction (RT-PCR) results at initial testing for suspicion of SARS-CoV-2 infection, and found to be positive in a subsequent test, are considered as RT-PCR false-negative cases. False-
I. Arévalo-Rodriguez, Diana Buitrago-García, Daniel Simancas-Racines, Paula Zambrano-Achig, R. del Campo, A. Ciapponi, O. Sued, L. Martínez-García, A. Rutjes, N. Low, J. Pérez-Molina, J. Zamora   +11 more
semanticscholar   +1 more source