Results 31 to 40 of about 2,171,089 (338)
Viral Dynamics and Real-Time RT-PCR Ct Values Correlation with Disease Severity in COVID-19
Diagnostics, 2021 Real-time RT-PCR is considered the gold standard confirmatory test for coronavirus disease 2019 (COVID-19). However, many scientists disagree, and it is essential to understand that several factors and variables can cause a false-negative test.
A. Rabaan +15 more
semanticscholar +1 more source
Veterinary Quarterly, 1998 A RT-PCR assay for the specific detection of RNA sequences from foot-and-mouth disease virus (FMDV) has been developed. The procedure permits also the detection of sequences that correlate with established FMDV serotypes. A computer program that allows selection of genotype-specific primers for RT-PCR amplification was used for the identification of ...
Dopazo, J. +7 more
openaire +4 more sources
Foods, 2021 Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante +4 more
doaj +1 more source
Real-time quantification of microRNAs by stem–loop RT–PCR
Nucleic Acids Research, 2005 A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity.
Caifu Chen +12 more
semanticscholar +1 more source
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
Journal of Molecular Endocrinology, 2002 The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology.
S. Bustin
semanticscholar +1 more source
Radiology, 2020 nucleic acid assay was also negative at this time. Six days after admission, the third RT-PCR 2019-nCoV nucleic acid assay was finally found to be positive. When specimen tests are negative, the possibility of a false-negative result should be considered
Peikai Huang +8 more
semanticscholar +1 more source
False negative of RT‐PCR and prolonged nucleic acid conversion in COVID‐19: Rather than recurrence
Journal of Medical Virology, 2020 A novel coronavirus (COVID-19) pandemic cause by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) threatens the world. We read with interest the recent report by Li et al.
Ai Tang Xiao, Y. Tong, S. Zhang
semanticscholar +1 more source
A novel method for real time quantitative RT-PCR.
Genome Research, 1996 A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed.
U E Gibson, C. Heid, P. M. Williams
semanticscholar +1 more source
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Nature Communications, 2020 Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction ...
Ioanna Smyrlaki +12 more
semanticscholar +1 more source
Quantitative PCR and RT-PCR in virology. [PDF]
Genome Research, 1993 In the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in ...
CLEMENTI M +5 more
openaire +4 more sources