Results 31 to 40 of about 2,220,062 (335)
JAMA Internal Medicine, 2022 Importance As self-collected home antigen tests become widely available, a better understanding of their performance during the course of SARS-CoV-2 infection is needed.
V. Chu +142 more
semanticscholar +1 more source
, 2011 In situ polymerase chain reaction (PCR) is a histological technique that exploits the advantages of PCR for detection of mRNA directly in tissue sections. It somehow conjugates together PCR and in situ hybridization that is more traditionally employed for mRNA localization in cell organelles, intact cells, or tissue sections. This chapter describes the
LOSSI, Laura +3 more
openaire +3 more sources
Real-time RT-PCR in COVID-19 detection: issues affecting the results
Expert Review of Molecular Diagnostics, 2020 Due to the rapid spread and increasing number of coronavirus disease 19 (COVID-19) cases caused by a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), rapid and accurat...
A. Tahamtan, A. Ardebili
semanticscholar +1 more source
PLoS ONE, 2021 At the start of the COVID-19 pandemic, the Centers for Disease Control and Prevention (CDC) designed, manufactured, and distributed the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel for SARS-CoV-2 detection.
Justin Lee +24 more
semanticscholar +1 more source
Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA
BioTechniques, 1998 The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps.
L.L. Hall +5 more
doaj +1 more source
False-negative results of initial RT-PCR assays for COVID-19: A systematic review
medRxiv, 2020 Background: Cases with negative reverse transcription-polymerase chain reaction (RT-PCR) results at initial testing for suspicion of SARS-CoV-2 infection, and found to be positive in a subsequent test, are considered as RT-PCR false-negative cases. False-
I. Arévalo-Rodriguez +11 more
semanticscholar +1 more source
Viral Dynamics and Real-Time RT-PCR Ct Values Correlation with Disease Severity in COVID-19
Diagnostics, 2021 Real-time RT-PCR is considered the gold standard confirmatory test for coronavirus disease 2019 (COVID-19). However, many scientists disagree, and it is essential to understand that several factors and variables can cause a false-negative test.
A. Rabaan +15 more
semanticscholar +1 more source
Foods, 2021 Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante +4 more
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Real-time quantification of microRNAs by stem–loop RT–PCR
Nucleic Acids Research, 2005 A novel microRNA (miRNA) quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones in terms of RT efficiency and specificity.
Caifu Chen +12 more
semanticscholar +1 more source
Journal of Medical Virology, 2020 In this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR) testing ...
Yafang Li +6 more
semanticscholar +1 more source