Results 41 to 50 of about 2,220,062 (335)
Radiology, 2020 nucleic acid assay was also negative at this time. Six days after admission, the third RT-PCR 2019-nCoV nucleic acid assay was finally found to be positive. When specimen tests are negative, the possibility of a false-negative result should be considered
Peikai Huang +8 more
semanticscholar +1 more source
False negative of RT‐PCR and prolonged nucleic acid conversion in COVID‐19: Rather than recurrence
Journal of Medical Virology, 2020 A novel coronavirus (COVID-19) pandemic cause by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) threatens the world. We read with interest the recent report by Li et al.
Ai Tang Xiao, Y. Tong, S. Zhang
semanticscholar +1 more source
A novel method for real time quantitative RT-PCR.
Genome Research, 1996 A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed.
U E Gibson, C. Heid, P. M. Williams
semanticscholar +1 more source
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Nature Communications, 2020 Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction ...
Ioanna Smyrlaki +12 more
semanticscholar +1 more source
Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]
Archives of Biological Sciences, 2012 Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D. +6 more
doaj +1 more source
Detection of Novel Coronavirus by RT-PCR in Stool Specimen from Asymptomatic Child, China
Emerging Infectious Diseases, 2020 We report an asymptomatic child who was positive for a coronavirus by reverse transcription PCR in a stool specimen 17 days after the last virus exposure. The child was virus positive in stool specimens for at least an additional 9 days.
A. Tang +10 more
semanticscholar +1 more source
An optimized protocol for stepwise optimization of real-time RT-PCR analysis
Horticulture Research, 2021 Computational tool-assisted primer design for real-time reverse transcription (RT) PCR (qPCR) analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome.
Fangzhou Zhao +11 more
semanticscholar +1 more source
Molekularbiologische Diagnostik von Pflanzenkrankkeiten
Julius-Kühn-Archiv, 2014 In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine +3 more
doaj +1 more source
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2006 Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV)
A. Cortez +8 more
doaj +1 more source
Virology Journal, 2020 Background The Coronavirus disease 2019 (COVID-19) pandemic continues to spread across the world. Hence, there is an urgent need for rapid, simple, and accurate tests to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection ...
Chutikarn Chaimayo +14 more
semanticscholar +1 more source