Results 41 to 50 of about 2,171,089 (338)

Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples [PDF]

, 2014
Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more
Reena Ghildyal, Xuelian Yu, Xuelian Yu
openaire   +3 more sources

Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]

Archives of Biological Sciences, 2012
Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D., Šekler M., Polaček V., Vasković N., Ašanin Ružica, Milić N., Nišavić J.   +6 more
doaj   +1 more source

Detection of Novel Coronavirus by RT-PCR in Stool Specimen from Asymptomatic Child, China

Emerging Infectious Diseases, 2020
We report an asymptomatic child who was positive for a coronavirus by reverse transcription PCR in a stool specimen 17 days after the last virus exposure. The child was virus positive in stool specimens for at least an additional 9 days.
A. Tang, Z. Tong, Hongling Wang, Yaxin Dai, Kefeng Li, Jie-nan Liu, Wen-jie Wu, Chen Yuan, Meng-lu Yu, Peng Li, Jian-Bo Yan   +10 more
semanticscholar   +1 more source

Molekularbiologische Diagnostik von Pflanzenkrankkeiten

Julius-Kühn-Archiv, 2014
In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine, Gottsberger, Richard A., Leichtfried, Thomas, Reisenzein, Helga   +3 more
doaj   +1 more source

Preanalytical Issues and Cycle Threshold Values in SARS-CoV-2 Real-Time RT-PCR Testing: Should Test Results Include These?

ACS Omega, 2021
Since the emergence of SARS-CoV-2 pandemic, clinical laboratories worldwide are overwhelmed with SARS-CoV-2 testing using the current gold standard: real-time reverse-transcription polymerase chain reaction (RT-PCR) assays. The large numbers of suspected
I. Engelmann, E. K. Alidjinou, J. Ogiez, Quentin Pagneux, Sana Miloudi, Ilyes Benhalima, Mahdi Ouafi, F. Sane, D. Hober, A. Roussel, C. Cambillau, D. Devos, R. Boukherroub, S. Szunerits   +13 more
semanticscholar   +1 more source

Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal Detection of Brucella spp., Leptospira spp., bovine herpesvirus and bovine viral diarrhea virus nucleic acids in aborted fetuses and bovines dead perinatal

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2006
Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV)
A. Cortez, A.M.G. Castro, M.B. Heinemann, R.M. Soares, R.C. Leite, E. Scarcelli, M.E. Genovez, A.A. Alfieri, L.J. Richtzenhain   +8 more
doaj   +1 more source

Rapid SARS-CoV-2 antigen detection assay in comparison with real-time RT-PCR assay for laboratory diagnosis of COVID-19 in Thailand

Virology Journal, 2020
Background The Coronavirus disease 2019 (COVID-19) pandemic continues to spread across the world. Hence, there is an urgent need for rapid, simple, and accurate tests to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection ...
Chutikarn Chaimayo, Bualan Kaewnaphan, Nattaya Tanlieng, N. Athipanyasilp, R. Sirijatuphat, M. Chayakulkeeree, N. Angkasekwinai, R. Sutthent, Nattawut Puangpunngam, Theerawoot Tharmviboonsri, O. Pongraweewan, S. Chuthapisith, Y. Sirivatanauksorn, W. Kantakamalakul, N. Horthongkham   +14 more
semanticscholar   +1 more source

Should RT‐PCR be considered a gold standard in the diagnosis of COVID‐19?

Journal of Medical Virology, 2020
To face the new Covid-19 pandemic, the need for early and accurate diagnosis of the disease among suspected cases quickly became obvious for effective management, and for better control of the spread of the disease in the population.
M. Dramé, M. T. Teguo, E. Proye, Fanny Hequet, M. Hentzien, L. Kanagaratnam, L. Godaert   +6 more
semanticscholar   +1 more source

Detection and identification of Fabavirus species by one-step RT-PCR and multiplex RT-PCR

Journal of Virological Methods, 2014
The genus Fabavirus of the family Secoviridae comprises a group of poorly characterized viruses. To date, only five species have been described: Broad bean wilt virus 1 (BBWV-1), Broad bean wilt virus 2 (BBWV-2), Lamium mild mosaic virus (LMMV), Gentian mosaic virus (GeMV) and Cucurbit mild mosaic virus (CuMMV).
Panno, S, Ferriol, I, Rangelm E, Olmosm, A, Han, C. G, MARTINELLI, Federico, Rubio, L, DAVINO, Salvatore   +7 more
openaire   +5 more sources

A case report of COVID-19 with false negative RT-PCR test: necessity of chest CT

Japanese Journal of Radiology, 2020
The definite diagnosis of corona virus disease 2019 (COVID-19) is based on the viral isolation or positive result of polymerase chain reaction (PCR) from sputum, or nasal swab, or throat swab. However, the sensitivity to detect COVID-19 of real time (RT)-
Hao Feng, Yujian Liu, M. Lv, Jianquan Zhong   +3 more
semanticscholar   +1 more source