Results 161 to 170 of about 616,892 (193)

α-Synuclein Seeding Assay Using RT-QuIC

2021
Synucleinopathies are neurodegenerative diseases that are associated with the misfolding and aggregation of α-synuclein (αSyn). They include Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy. In each disease, it has been proposed that aggregates of αSyn represent different conformational strains of αSyn, leading to self ...
Ayami, Okuzumi, Taku, Hatano, Takeshi, Fukuhara, Shinichi, Ueno, Nobuyuki, Nukina, Yuzuru, Imai, Nobutaka, Hattori   +6 more
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Alpha‐Synuclein RT‐QuIC in Idiopathic Normal Pressure Hydrocephalus

Annals of Neurology, 2022
This study quantified the occurrence of an underlying synucleinopathy in 50 patients with idiopathic normal pressure hydrocephalus by means of real‐time quaking‐induced conversion, a highly sensitive and specific technique capable of detecting and amplifying misfolded aggregated forms of α‐synuclein in the cerebrospinal fluid.
Alfonso Fasano, Ivan Martinez‐Valbuena, Paula Azevedo, Carolina Candeias da Silva, Musleh Algarni, Anna Vasilevskaya, Chloe Anastassiadis, Foad Taghdiri, Paul Kongkham, Ivan Radovanovic, Gelareh Zadeh, Anthony E. Lang, David F. Tang‐Wai, Gabor G. Kovacs, Maria Carmela Tartaglia   +14 more
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RT-QuIC as ultrasensitive method for prion detection

Cell and Tissue Research, 2022
Real-time quaking-induced conversion (RT-QuIC) is a cell-free abnormal form of prion protein (PrPSc) amplification method using recombinant prion protein from Escherichia coli that can measure prion seeding activity in samples with high sensitivity. The advantages of this method are that it is much more sensitive than Western blotting, which is usually
openaire   +2 more sources

Advancing Prion Diagnostics: RT-QuIC Applications in Peripheral Tissues.

Sub-cellular biochemistry
Prion diseases (PrDs) are fatal neurodegenerative disorders characterized by the accumulation of misfolded prion protein (PrPSc) in the central nervous system (CNS). This pathological isoform of the cellular prion protein drives disease pathogenesis through its unique ability to propagate itself via a template-directed misfolding mechanism.
Merve Begüm Bacınoğlu, Giuseppe Bufano, F. Cazzaniga, G. Zanusso, Giuseppe Legname, F. Moda   +5 more
semanticscholar   +2 more sources

A meta-analysis on RT-QuIC for the diagnosis of sporadic CJD

Acta Neurologica Belgica, 2021
Creutzfeld-Jakob disease (CJD) is a fatal neurodegenerative disease which belongs to the family of transmissible spongiform encephalopathies (TSEs), or prion diseases. Historically, CJD diagnosis has been based on the combination of clinical features and in vivo markers, including CSF protein assays, MRI and EEG changes.
Simela Chatzikonstantinou, Dimitrios Kazis, Eleni Karantali, Mark Knights, Jack McKenna, Foivos Petridis, Ioannis Mavroudis   +6 more
openaire   +2 more sources

Seed amplification and RT-QuIC assays to investigate protein seed structures and strains

Cell and Tissue Research, 2022
The accumulation of misfolded proteins as amyloid fibrils in the brain is characteristic of most neurodegenerative disorders. These misfolded proteins are capable of self-amplifying through protein seeding mechanisms, leading to accumulation in the host. First shown for PrP prions and prion diseases, it is now recognized that self-propagating misfolded
Heidi G. Standke, Allison Kraus
semanticscholar   +3 more sources

Prevalence and clinical impact of alpha-synuclein pathology in idiopathic normal pressure hydrocephalus: Insights from RT-QuIC assay.

Parkinsonism & Related Disorders
BACKGROUND The relationship between idiopathic normal pressure hydrocephalus (iNPH) and synucleinopathy remains unclear. This study aimed to explore the prevalence of α-synuclein pathology in iNPH patients and its impact on clinical outcomes.
Je kook Yu, Hye Joung Choi, Dongwoo Kim, P. Ngoc, I. Kwak, Huu Dat Nguyen, Trung Nguyen Thanh, Suk Jun Song, Jeongjae Lee, Hyeo-il Ma, Young Eun Kim   +10 more
semanticscholar   +1 more source

RT-QuIC Assays for Prion Disease Detection and Diagnostics

2017
In coping with prion diseases, it is important to have tests that are practical enough for routine applications in medicine, agriculture, wildlife biology, and research, yet sensitive enough to detect minimal amounts of infectivity. Real-time quaking-induced conversion (RT-QuIC) assays have evolved to the point where they fulfill these criteria in ...
Christina D, Orrù, Bradley R, Groveman, Andrew G, Hughson, Matteo, Manca, Lynne D, Raymond, Gregory J, Raymond, Katrina J, Campbell, Kelsie J, Anson, Allison, Kraus, Byron, Caughey   +9 more
openaire   +2 more sources

Detection and Diagnosis of Prion Diseases Using RT-QuIC: An Update

2017
Until recently, it has been difficult to detect all infectious levels of prions and diagnose prion diseases in living humans and other animals. Real-time quaking-induced conversion (RT-QuIC), an ultrasensitive test based on amplification of the amyloid seeding activity of prions, is now achieving the sensitivity and practicality required for routine ...
Caughey, Byron, Orru, Christina D., Groveman, Bradley R., Bongianni, Matilde, Hughson, Andrew G., Raymond, Lynne D., Manca, Matteo, Kraus, Allison, Raymond, Gregory J., Fiorini, Michele, Pocchiari, Maurizio, Zanusso, Gianluigi   +11 more
openaire   +1 more source

A systematic review comparing the diagnostic value of 14-3-3 protein in the cerebrospinal fluid, RT-QuIC and RT-QuIC on nasal brushing in sporadic Creutzfeldt–Jakob disease

Acta Neurologica Belgica, 2018
Sporadic Creutzfeldt-Jakob disease (sCJD) is a human prion disease that is a relatively common differential diagnosis in dementia patients. Therefore it needs a good diagnostic tool. Brain autopsy is the golden standard for the diagnosis of CJD; however, a less invasive technique is 14-3-3 protein measurement in the cerebrospinal fluid (CSF).
Olivia Behaeghe, Elias Mangelschots, Bart De Vil, Patrick Cras   +3 more
openaire   +3 more sources