Optimization of the Real-Time Quaking-Induced Conversion Assay for Prion Disease Diagnosis [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 The real-time quaking-induced conversion (RT-QuIC) assay is a highly reproducible and robust methodology exhibiting an excellent pre-mortem diagnostic accuracy for prion diseases.
Inga Zerr +11 more
doaj +7 more sources
The Use of Real-Time Quaking-Induced Conversion for the Diagnosis of Human Prion Diseases [PDF]
Frontiers in Aging Neuroscience, 2022 Prion diseases are rapidly progressive, invariably fatal, transmissible neurodegenerative disorders associated with the accumulation of the amyloidogenic form of the prion protein in the central nervous system (CNS).
Anna Poleggi +5 more
doaj +5 more sources
Real-Time Quaking- Induced Conversion Assays for Prion Diseases, Synucleinopathies, and Tauopathies [PDF]
Frontiers in Aging Neuroscience, 2022 Prion diseases, synucleinopathies and tauopathies are neurodegenerative disorders characterized by deposition of abnormal protein aggregates in brain and other tissues.
Sarah Vascellari +2 more
doaj +2 more sources
Potential sources of interference with the highly sensitive detection and quantification of alpha‐synuclein seeds by qRT‐QuIC [PDF]
FEBS Open Bio, 2020 Parkinson’s disease (PD) is a progressive neurodegenerative disease which is histologically characterized by loss of dopaminergic neurons in the substantia nigra and deposition of aggregated alpha‐synuclein (aSyn) in the brain.
Viktoria C. Ruf +7 more
doaj +2 more sources
Evaluation of Real-Time Quaking-Induced Conversion, ELISA, and Immunohistochemistry for Chronic Wasting Disease Diagnosis [PDF]
Frontiers in Veterinary Science, 2022 Chronic wasting disease (CWD) is a transmissible prion disorder, primarily affecting free-ranging and captive cervids in North America (United States and Canada), South Korea, and Europe (Finland, Norway, and Sweden).
Carine L. Holz +6 more
doaj +2 more sources
Development of a sensitive real-time quaking-induced conversion (RT-QuIC) assay for application in prion-infected blood [PDF]
PLoS ONE, 2023 Efforts to prevent human-to-human transmission of variant Creutzfeldt-Jakob disease (vCJD) by contaminated blood would be aided by the development of a sensitive diagnostic test that could be routinely used to screen blood donations.
Charlotte M. Thomas +7 more
doaj +4 more sources
Defining the Protein Seeds of Neurodegeneration using Real-Time Quaking-Induced Conversion Assays [PDF]
Biomolecules, 2020 Neurodegenerative diseases are characterized by the accumulation of disease-related misfolded proteins. It is now widely understood that the characteristic self-amplifying (i.e., seeding) capacity once only attributed to the prions of transmissible ...
Matteo Manca, Allison Kraus
doaj +2 more sources
Improved Real-Time Quaking Induced Conversion for Early Diagnostics of Creutzfeldt-Jakob Disease in Denmark. [PDF]
Int J Mol Sci, 2023 Cerebrospinal fluid-based real-time quaking-induced conversion (CSF RT-QuIC) is currently the most prominent method for early detection of sporadic Creutzfeldt–Jakob disease (sCJD), the most common prion disease.
Bsoul R +6 more
europepmc +4 more sources
Development of α-Synuclein Real-Time Quaking-Induced Conversion as a Diagnostic Method for α-Synucleinopathies [PDF]
Frontiers in Aging Neuroscience, 2021 Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy are characterized by aggregation of abnormal α-synuclein (α-syn) and collectively referred to as α-synucleinopathy. Because these diseases have different prognoses and treatments,
Takehiro Nakagaki +2 more
doaj +2 more sources
Evaluation of Winter Ticks (Dermacentor albipictus) Collected from North American Elk (Cervus canadensis) in an Area of Chronic Wasting Disease Endemicity for Evidence of PrP CWD Amplification Using Real-Time Quaking-Induced Conversion Assay [PDF]
mSphere, 2021 This study reports the first finding of detectable levels of prions linked to chronic wasting disease in a tick collected from a clinically infected elk.
N. J. Haley +4 more
doaj +2 more sources