Results 1 to 10 of about 10,380 (154)

Optimization of the Real-Time Quaking-Induced Conversion Assay for Prion Disease Diagnosis [PDF]

Frontiers in Bioengineering and Biotechnology, 2020
The real-time quaking-induced conversion (RT-QuIC) assay is a highly reproducible and robust methodology exhibiting an excellent pre-mortem diagnostic accuracy for prion diseases.
Inga Zerr, Maria Cramm, Susana Margarida da Silva Correia, Saima Zafar, Saima Zafar, Anna Villar-Piqué, Anna Villar-Piqué, Anna Villar-Piqué, Franc Llorens, Franc Llorens, Franc Llorens, Matthias Schmitz   +11 more
doaj   +12 more sources

Real-Time Quaking- Induced Conversion Assays for Prion Diseases, Synucleinopathies, and Tauopathies [PDF]

Frontiers in Aging Neuroscience, 2022
Prion diseases, synucleinopathies and tauopathies are neurodegenerative disorders characterized by deposition of abnormal protein aggregates in brain and other tissues.
Sarah Vascellari, Christina D. Orrù, Byron Caughey   +2 more
doaj   +6 more sources

The Use of Real-Time Quaking-Induced Conversion for the Diagnosis of Human Prion Diseases [PDF]

Frontiers in Aging Neuroscience, 2022
Prion diseases are rapidly progressive, invariably fatal, transmissible neurodegenerative disorders associated with the accumulation of the amyloidogenic form of the prion protein in the central nervous system (CNS).
Anna Poleggi, Simone Baiardi, Simone Baiardi, Anna Ladogana, Piero Parchi, Piero Parchi   +5 more
doaj   +6 more sources

Defining the Protein Seeds of Neurodegeneration using Real-Time Quaking-Induced Conversion Assays [PDF]

Biomolecules, 2020
Neurodegenerative diseases are characterized by the accumulation of disease-related misfolded proteins. It is now widely understood that the characteristic self-amplifying (i.e., seeding) capacity once only attributed to the prions of transmissible ...
Matteo Manca, Allison Kraus
doaj   +6 more sources

Improved Real-Time Quaking Induced Conversion for Early Diagnostics of Creutzfeldt-Jakob Disease in Denmark. [PDF]

Int J Mol Sci, 2023
Cerebrospinal fluid-based real-time quaking-induced conversion (CSF RT-QuIC) is currently the most prominent method for early detection of sporadic Creutzfeldt–Jakob disease (sCJD), the most common prion disease.
Bsoul R, Lund EL, Burns K, Andrews M, McKenzie N, Green A, Areškevičiūtė A.   +6 more
europepmc   +9 more sources

Development of a sensitive real-time quaking-induced conversion (RT-QuIC) assay for application in prion-infected blood [PDF]

PLoS ONE, 2023
Efforts to prevent human-to-human transmission of variant Creutzfeldt-Jakob disease (vCJD) by contaminated blood would be aided by the development of a sensitive diagnostic test that could be routinely used to screen blood donations.
Charlotte M. Thomas, M. Khalid F. Salamat, Christopher de Wolf, Sandra McCutcheon, A. Richard Alejo Blanco, Jean C. Manson, Nora Hunter, E. Fiona Houston   +7 more
doaj   +9 more sources

Real-Time Quaking-Induced Conversion Detection of Bovine Spongiform Encephalopathy Prions in a Subclinical Steer [PDF]

Frontiers in Veterinary Science, 2018
Bovine spongiform encephalopathy (BSE) belongs to a group of fatal prion diseases that result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that accumulates in the brain.
Soyoun Hwang, M. Heather West Greenlee, Anne Balkema-Buschmann, Martin H. Groschup, Eric M. Nicholson, Justin J. Greenlee   +5 more
doaj   +6 more sources

Concordance of cerebrospinal fluid real-time quaking-induced conversion across the European Creutzfeldt-Jakob Disease Surveillance Network. [PDF]

Eur J Neurol, 2022
Background and purpose: Cerebrospinal fluid (CSF) real-time quaking-induced conversion (RT-QuIC) has a high degree of sensitivity and specificity for the diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD) and this has led to its being included in ...
McKenzie N, Piconi G, Culeux A, Hammarin AL, Stergiou C, Tzartos S, Versleijen AAM, van de Geer J, Cras P, Cardone F, Ladogana A, Mannana A, Rossi M, Bongianni M, Perra D, Regelsberger G, Klotz S, Hornemann S, Aguzzi A, Schmitz M, Andrews M, Burns K, Haïk S, Ruiz-García R, Verner-Carlsson J, Tzartos J, Verbeek MM, De Vil B, Poleggi A, Parchi P, Zanusso G, Gelpi E, Frontzek K, Reimann R, Hermann P, Zerr I, Pal S, Green A.   +37 more
europepmc   +7 more sources

Sporadic Creutzfeldt-Jakob disease: Real-Time Quaking Induced Conversion (RT-QuIC) assay represents a major diagnostic advance [PDF]

European Journal of Histochemistry, 2021
Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare and fatal neurodegenerative disorder with an incidence of 1.5 to 2 cases per million population/year. The disease is caused by a proteinaceous infectious agent, named prion (or PrPSc), which arises from
Federico Angelo Cazzaniga, Edoardo Bistaffa, Chiara Maria Giulia De Luca, Giuseppe Bufano, Antonio Indaco, Giorgio Giaccone, Fabio Moda   +6 more
doaj   +4 more sources

TDP-43 real-time quaking induced conversion reaction optimization and detection of seeding activity in CSF of amyotrophic lateral sclerosis and frontotemporal dementia patients. [PDF]

Brain Commun, 2020
The pathological deposition of the transactive response DNA-binding protein of 43 kDa occurs in the majority (∼97%) of amyotrophic lateral sclerosis and in around 45% of frontotemporal lobar degeneration cases.
Scialò C, Tran TH, Salzano G, Novi G, Caponnetto C, Chiò A, Calvo A, Canosa A, Moda F, Caroppo P, Silani V, Ticozzi N, Ratti A, Borroni B, Benussi L, Ghidoni R, Furlanis G, Manganotti P, Senigagliesi B, Parisse P, Brasselet R, Buratti E, Legname G.   +22 more
europepmc   +5 more sources