Premortem diagnosis of pathologically confirmed sporadic Creutzfeldt‐Jakob disease
Clinical Case Reports, 2021 Sporadic Creutzfeldt‐Jakob disease should be considered in any case of rapid neuropsychiatric decline. While neuropathological examination of a brain biopsy specimen remains the only definitive diagnostic method and real‐time quaking‐induced conversion ...
Elina T Ziukelis +2 more
doaj +1 more source
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material. [PDF]
J Vis Exp, 2017 The RT-QuIC technique is a sensitive in vitro cell-free prion amplification assay based mainly on the seeded misfolding and aggregation of recombinant prion protein (PrP) substrate using prion seeds as a template for the conversion. RT-QuIC is a novel high-throughput technique which is analogous to real-time polymerase chain reaction (PCR).
Cheng YC +5 more
europepmc +4 more sources
Transmission of CJD from nasal brushings but not spinal fluid or RT‐QuIC product
Annals of Clinical and Translational Neurology, 2020 Objective The detection of prion seeding activity in CSF and olfactory mucosal brushings using real‐time quaking‐induced conversion assays allows highly accurate clinical diagnosis of sporadic Creutzfeldt–Jakob disease.
Gregory J. Raymond +13 more
doaj +1 more source
Toward an Early Real-Time Quaking-Induced Conversion-Based Diagnostic Biomarker for Lewy Body-Related Synucleinopathies. [PDF]
Mov Disord Clin Pract, 2020 [no abstract available]
Magrinelli F, Tinazzi M, Bhatia KP.
europepmc +6 more sources
Molecular Neurodegeneration, 2021 Background Detection of the pathological and disease-associated alpha-synuclein (αSynD) in the brain is required to formulate the definitive diagnosis of multiple system atrophy (MSA) and Parkinson’s disease (PD).
Connor Bargar +18 more
doaj +1 more source
An autopsy-verified case of steroid-responsive encephalopathy with convulsion and a false-positive result from the real-time quaking-induced conversion assay [PDF]
Prion, 2017 Katsuya Satoh +2 more
exaly +2 more sources
Real time-quaking induced conversion assay (RT-QUIC) v1
, 2022 Seeded-amplification assay (SAA) method for the detection of pathology-associated aggregation-templating competent forms of alpha-synuclein and other interacting factors.
andrew.west west, Arpine Sokratian
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Real time quaking‐induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt–Jakob disease [PDF]
Annals of Neurology, 2012 AbstractObjective:Current cerebrospinal fluid (CSF) tests for sporadic Creutzfeldt–Jakob disease (sCJD) are based on the detection of surrogate markers of neuronal damage such as CSF 14‐3‐3, which are not specific for sCJD. A number of prion protein conversion assays have been developed, including real time quaking‐induced conversion (RT‐QuIC).
McGuire, Lynne I +11 more
openaire +3 more sources
Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC
BMC Research Notes, 2018 Objective Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases, often referred as prion diseases. TSEs result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that ...
Soyoun Hwang +3 more
doaj +1 more source
Atypical Case of VV1 Creutzfeldt–Jakob Disease Subtype: Case Report
Frontiers in Neurology, 2022 Creutzfeldt–Jakob disease (CJD) is a rare form of rapidly progressive, neurodegenerative disease that results from the misfolding and accumulation of an aberrant, disease-associated prion protein (PrPD).
Adrianna E. Carrasco +3 more
doaj +1 more source