Aqueous extraction of formalin-fixed paraffin-embedded tissue and detection of prion disease using real-time quaking-induced conversion [PDF]
BMC Research NotesObjective The goal of the research presented here is to determine if methods previously developed for the aqueous extraction of PrPSc from formalin-fixed paraffin-embedded tissue (FFPET) are applicable to the detection PrPSc by real-time quaking induced ...
Eric M. Nicholson +2 more
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Involvement of N- and C-terminal region of recombinant cervid prion protein in its reactivity to CWD and atypical BSE prions in real-time quaking-induced conversion reaction in the presence of high concentrations of tissue homogenates. [PDF]
Prion, 2020 Suzuki A +6 more
europepmc +2 more sources
Chinese Journal of Contemporary Neurology and Neurosurgery, 2023 Creutzfeldt⁃Jakob disease (CJD) is a group of clinically rare neurodegenerative diseases caused by misfolding of prion proteins, and is the most common type of human prion diseases.
Dian HE
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Premortem diagnosis of pathologically confirmed sporadic Creutzfeldt‐Jakob disease
Clinical Case Reports, 2021 Sporadic Creutzfeldt‐Jakob disease should be considered in any case of rapid neuropsychiatric decline. While neuropathological examination of a brain biopsy specimen remains the only definitive diagnostic method and real‐time quaking‐induced conversion ...
Elina T Ziukelis +2 more
doaj +1 more source
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material. [PDF]
J Vis Exp, 2017 The RT-QuIC technique is a sensitive in vitro cell-free prion amplification assay based mainly on the seeded misfolding and aggregation of recombinant prion protein (PrP) substrate using prion seeds as a template for the conversion. RT-QuIC is a novel high-throughput technique which is analogous to real-time polymerase chain reaction (PCR).
Cheng YC +5 more
europepmc +4 more sources
Transmission of CJD from nasal brushings but not spinal fluid or RT‐QuIC product
Annals of Clinical and Translational Neurology, 2020 Objective The detection of prion seeding activity in CSF and olfactory mucosal brushings using real‐time quaking‐induced conversion assays allows highly accurate clinical diagnosis of sporadic Creutzfeldt–Jakob disease.
Gregory J. Raymond +13 more
doaj +1 more source
Toward an Early Real-Time Quaking-Induced Conversion-Based Diagnostic Biomarker for Lewy Body-Related Synucleinopathies. [PDF]
Mov Disord Clin Pract, 2020 [no abstract available]
Magrinelli F, Tinazzi M, Bhatia KP.
europepmc +6 more sources
An autopsy-verified case of steroid-responsive encephalopathy with convulsion and a false-positive result from the real-time quaking-induced conversion assay. [PDF]
Prion, 2017 Hayashi Y +9 more
europepmc +2 more sources
, 2022 Disagreement between postmortem immunohistochemistry (IHC) and real-time quaking-induced conversion (RT-QuIC) results.
Stuart S. Lichtenberg (14129070) +5 more
core +1 more source
Detection of prions in matching post-mortem skin and cerebrospinal fluid samples using second-generation real-time quaking-induced conversion assay. [PDF]
Sci RepReal-time quaking-induced conversion assay (RT-QuIC) exploits templating activity of pathogenic prion protein for ultrasensitive detection of prions. We have utilized second generation RT-QuIC assay to analyze matching post-mortem cerebrospinal fluid and
Baranová S +7 more
europepmc +2 more sources