Results 31 to 40 of about 10,380 (154)

Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic Creutzfeldt-Jakob disease : an international study [PDF]

Annals of Neurology, 2016
: Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be ...
Cheng , Keding, Cras, Patrick, Shi , Song, de Vil , Bart, Poggiolini , Ilaria, Atarashi , Ryuichiro, Grznarova, Katarina, Fairfoul, G, Cramm, Maria, Haïk, S, Cras , Patrick, Knox , David, Collins, Steven J., Green, A, Equestre, Michele, Cramm , Maria, Haik, Stephane, Satoh, K, Shi, Song, Sarros , Shannon, McGuire, LI, de Vil, B, Tagliavini, Fabrizio, Equestre, M, Pocchiari, Maurizio, Fairfoul, Graham, Sarros, Shannon, Parchi, Piero, Green, Alison, Fairfoul , Graham ; id_orcid, Schmitz, Matthias, De Vil, Bart, Zerr, Inga, Shi, S, Collins, S, McGuire, Lynne I., Suardi, Silvia, Poleggi, Anna, Poggiolini, I, Poggiolini, Ilaria, Knox, David, Atarashi, Ryuichiro, Cheng, Keding, Grznarova, K, Knox, D, Tagliavini, F, Grznarova , Katarina, Collins, Steven, Suardi, S, Cramm, M, Sarros, S, Parchi, P, Poleggi, A, Cras, P, Zerr, I, Schmitz, M, Pocchiari, M, Satoh, Katsuya, Cheng, K, Atarashi, R   +59 more
core   +3 more sources

quicR: An R library for streamlined data handling of real-time quaking induced conversion assays

SoftwareX
Real-time quaking induced conversion (RT-QuIC) has become a valuable diagnostic tool for protein misfolding disorders such as Creutzfeldt–Jakob disease and Parkinson’s disease.
Gage R. Rowden, Peter A. Larsen
doaj   +2 more sources

Detection of prions in matching post-mortem skin and cerebrospinal fluid samples using second-generation real-time quaking-induced conversion assay. [PDF]

Sci Rep
Real-time quaking-induced conversion assay (RT-QuIC) exploits templating activity of pathogenic prion protein for ultrasensitive detection of prions. We have utilized second generation RT-QuIC assay to analyze matching post-mortem cerebrospinal fluid and
Baranová S, Moško T, Brůžová M, Haldiman T, Kim C, Safar JG, Matěj R, Holada K.   +7 more
europepmc   +2 more sources

Early and Non-Invasive Detection of Chronic Wasting Disease Prions in Elk Feces by Real-Time Quaking Induced Conversion. [PDF]

PLoS ONE, 2016
Chronic wasting disease (CWD) is a fatal prion disease of wild and captive cervids in North America. Prions are infectious agents composed of a misfolded version of a host-encoded protein, termed PrPSc.
Yo Ching Cheng, Samia Hannaoui, Theodore R John, Sandor Dudas, Stefanie Czub, Sabine Gilch   +5 more
doaj   +2 more sources

Aqueous extraction of formalin-fixed paraffin-embedded tissue and detection of prion disease using real-time quaking-induced conversion [PDF]

BMC Research Notes
Objective The goal of the research presented here is to determine if methods previously developed for the aqueous extraction of PrPSc from formalin-fixed paraffin-embedded tissue (FFPET) are applicable to the detection PrPSc by real-time quaking induced ...
Eric M. Nicholson, Justin J. Greenlee, Soyoun Hwang   +2 more
doaj   +2 more sources

Are We Ready for Detecting α-Synuclein Prone to Aggregation in Patients? The Case of "Protein-Misfolding Cyclic Amplification" and "Real-Time Quaking-Induced Conversion" as Diagnostic Tools. [PDF]

Front Neurol, 2018
The accumulation and deposition of α-synuclein aggregates in brain tissue is the main event in the pathogenesis of different neurodegenerative disorders grouped under the term of synucleinopathies.
Paciotti S, Bellomo G, Gatticchi L, Parnetti L.   +3 more
europepmc   +2 more sources

Real-time quaking-induced conversion [PDF]

Prion, 2011
We recently developed a new in vitro amplification technology, designated "real-time quaking-induced conversion (RT-QUIC)", for detection of the abnormal form of prion protein (PrPSc) in easily accessible specimens such as cerebrospinal fluid (CSF).
Ryuichiro Atarashi, Kazunori Sano, Katsuya Satoh, Noriyuki Nishida   +3 more
core   +3 more sources

Involvement of N- and C-terminal region of recombinant cervid prion protein in its reactivity to CWD and atypical BSE prions in real-time quaking-induced conversion reaction in the presence of high concentrations of tissue homogenates. [PDF]

Prion, 2020
Suzuki A, Sawada K, Yamasaki T, Denkers ND, Mathiason CK, Hoover EA, Horiuchi M.   +6 more
europepmc   +2 more sources

Application value of real ⁃ time quaking ⁃ induced conversion technology in clinical diagnosis of Creutzfeldt⁃Jakob disease

Chinese Journal of Contemporary Neurology and Neurosurgery, 2023
Creutzfeldt⁃Jakob disease (CJD) is a group of clinically rare neurodegenerative diseases caused by misfolding of prion proteins, and is the most common type of human prion diseases.
Dian HE
doaj   +1 more source

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material. [PDF]

J Vis Exp, 2017
The RT-QuIC technique is a sensitive in vitro cell-free prion amplification assay based mainly on the seeded misfolding and aggregation of recombinant prion protein (PrP) substrate using prion seeds as a template for the conversion. RT-QuIC is a novel high-throughput technique which is analogous to real-time polymerase chain reaction (PCR).
Cheng YC, Hannaoui S, John TR, Dudas S, Czub S, Gilch S.   +5 more
europepmc   +4 more sources