Results 11 to 20 of about 4,479 (143)

Sporadic Creutzfeldt-Jakob disease: Real-Time Quaking Induced Conversion (RT-QuIC) assay represents a major diagnostic advance [PDF]

European Journal of Histochemistry, 2021
Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare and fatal neurodegenerative disorder with an incidence of 1.5 to 2 cases per million population/year. The disease is caused by a proteinaceous infectious agent, named prion (or PrPSc), which arises from
Federico Angelo Cazzaniga, Edoardo Bistaffa, Chiara Maria Giulia De Luca, Giuseppe Bufano, Antonio Indaco, Giorgio Giaccone, Fabio Moda   +6 more
doaj   +2 more sources

Assessment of Real-Time Quaking-Induced Conversion (RT-QuIC) Assay, Immunohistochemistry and ELISA for Detection of Chronic Wasting Disease under Field Conditions in White-Tailed Deer: A Bayesian Approach [PDF]

Pathogens, 2022
Chronic wasting disease (CWD) is a transmissible prion disease of the cervidae family. ELISA and IHC tests performed postmortem on the medial retropharyngeal lymph nodes (RPLN) or obex are considered diagnostic gold standards for prion detection. However,
Catalina Picasso-Risso, Marc D. Schwabenlander, Gage Rowden, Michelle Carstensen, Jason C. Bartz, Peter A. Larsen, Tiffany M. Wolf   +6 more
doaj   +2 more sources

PAD-Beads enrichment enhances detection of PrPSc using real-time quaking-induced conversion [PDF]

BMC Research Notes, 2019
Objective Scrapie is a transmissible spongiform encephalopathy (TSE) that naturally occurs in sheep and goats. This fatal neurodegenerative disease results from misfolding of the normal cellular prion protein (PrPC) to a pathogenic prion protein form ...
Soyoun Hwang, Rohana P. Dassanayake, Eric M. Nicholson   +2 more
doaj   +2 more sources

Creutzfeldt-Jakob disease: recent developments [version 1; referees: 2 approved] [PDF]

F1000Research, 2017
Creutzfeldt-Jakob disease (CJD) is a rare prion disorder that has been the subject of both professional and public interest following the identification of variant CJD as a zoonotic disorder.
Graeme Mackenzie, Robert Will
doaj   +4 more sources

Practical Real-Time Quaking-Induced Conversion for Detecting Classical Bovine Spongiform Encephalopathy and Classical and Atypical Scrapie Prions [PDF]

Pathogens
Real-time quaking-induced conversion (RT-QuIC) is highly sensitive for prion detection; however, inhibitory factors present in tissue homogenates readily interfere with the assay.
Akio Suzuki, Kazuhei Sawada, Taku Nakashima, Toyotaka Sato, Kohtaro Miyazawa, Yuichi Matsuura, Keigo Ikeda, Yoshifumi Iwamaru, Motohiro Horiuchi   +8 more
doaj   +2 more sources

Real time quaking-induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt-Jakob disease [PDF]

Annals of Neurology, 2012
OBJECTIVE: Current cerebrospinal fluid (CSF) tests for sporadic Creutzfeldt-Jakob disease (sCJD) are based on the detection of surrogate markers of neuronal damage such as CSF 14-3-3 which are not specific for sCJD.
Andrews, Mary, Appleford, Nigel E, Caughey, Byron, Green, Alison J E, Head, Mark W, Knight, Richard S G, Mallinson, Gary, McGuire, Lynne I, Orrú, Christina D, Peden, Alexander H, Wilham, Jason M, Will, Robert G   +11 more
core   +3 more sources

Detection of prions in skin punch biopsies of Creutzfeldt–Jakob disease patients [PDF]

Annals of Clinical and Translational Neurology, 2020
Prion real‐time quaking‐induced conversion (RT‐QuIC) is an ultrasensitive assay detecting pathological aggregates of misfolded prion protein in biospecimens.
Angela Mammana, Simone Baiardi, Marcello Rossi, Alessia Franceschini, Vincenzo Donadio, Sabina Capellari, Byron Caughey, Piero Parchi   +7 more
doaj   +2 more sources

Progressive multifocal diffusion-weighted imaging hyperintensities in sporadic Creutzfeldt–Jakob disease with positive cerebrospinal fluid real-time quaking-induced conversion: a case report [PDF]

Journal of Medical Case Reports
Background Creutzfeldt–Jakob disease is a rare and invariably fatal neurodegenerative disorder classified among the prion diseases. Primarily affecting the brain, this condition precipitates neuronal cell demise, culminating in swiftly advancing dementia
Puyu Li, Wenjing Wang, Lingzhi Li, Keding Cheng, Ying Wang, Yuanyuan Li, Wei Xu   +6 more
doaj   +2 more sources

Early diagnosis of a case of Heidenhain variant of Creutzfeld-Jakob disease by cerebrospinal fluid real-time quaking-induced conversion test [PDF]

Prion
Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare, rapidly progressive, and fatal neurodegenerative disorder caused by the accumulation of abnormal prion proteins in the central nervous system. The Heidenhain variant (HvCJD), a rare subtype of sCJD, is
Lin Li, Rong Yan, Jiawei Wang, Yanjun Guo   +3 more
doaj   +2 more sources

Real-Time Quaking-Induced Conversion Detection of Bovine Spongiform Encephalopathy Prions in a Subclinical Steer [PDF]

Frontiers in Veterinary Science, 2018
Bovine spongiform encephalopathy (BSE) belongs to a group of fatal prion diseases that result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that accumulates in the brain.
Soyoun Hwang, M. Heather West Greenlee, Anne Balkema-Buschmann, Martin H. Groschup, Eric M. Nicholson, Justin J. Greenlee   +5 more
doaj   +2 more sources