Results 11 to 20 of about 2,160 (134)

PAD-Beads enrichment enhances detection of PrPSc using real-time quaking-induced conversion [PDF]

BMC Research Notes, 2019
Objective Scrapie is a transmissible spongiform encephalopathy (TSE) that naturally occurs in sheep and goats. This fatal neurodegenerative disease results from misfolding of the normal cellular prion protein (PrPC) to a pathogenic prion protein form ...
Soyoun Hwang, Rohana P. Dassanayake, Eric M. Nicholson   +2 more
doaj   +5 more sources

Real-Time Quaking-Induced Conversion Detection of Bovine Spongiform Encephalopathy Prions in a Subclinical Steer [PDF]

Frontiers in Veterinary Science, 2018
Bovine spongiform encephalopathy (BSE) belongs to a group of fatal prion diseases that result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that accumulates in the brain.
Soyoun Hwang, M. Heather West Greenlee, Anne Balkema-Buschmann, Martin H. Groschup, Eric M. Nicholson, Justin J. Greenlee   +5 more
doaj   +5 more sources

Improved Real-Time Quaking Induced Conversion for Early Diagnostics of Creutzfeldt–Jakob Disease in Denmark [PDF]

International Journal of Molecular Sciences, 2023
Cerebrospinal fluid-based real-time quaking-induced conversion (CSF RT-QuIC) is currently the most prominent method for early detection of sporadic Creutzfeldt–Jakob disease (sCJD), the most common prion disease. CSF RT-QuIC delivers high sensitivity (>90%) and specificity (100%), which has been demonstrated by large ring-trial studies testing ...
Eva Løbner Lund, Ausrine Areškevičiūtė, Alison Green   +2 more
exaly   +7 more sources

Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion [PDF]

BioMed Research International, 2017
Prions composed of pathogenic scrapie prion protein (PrPSc) are infectious pathogens that cause progressive neurological conditions known as prion diseases or transmissible spongiform encephalopathies. Although these diseases pose considerable risk to public health, procedures for early diagnosis have not been established.
Chongsuk Ryou, Hye-Mi Lee, Ryou Chongsuk   +2 more
exaly   +5 more sources

α‐Synuclein Real‐Time Quaking‐Induced Conversion in the Submandibular Glands of Parkinson's Disease Patients [PDF]

Movement Disorders, 2020
AbstractBackgroundIdentification of a peripheral biomarker is a major roadblock in the diagnosis of PD. Immunohistological identification of p‐serine 129 α‐synuclein in the submandibular gland tissues of PD patients has been recently reported.ObjectiveWe report on a proof‐of‐principle study for using an ultra‐sensitive and specific, real‐time quaking ...
Naveen Kondru, Anumantha Kanthasamy, Vellareddy Anantharam   +2 more
exaly   +5 more sources

Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies

Experimental Gerontology
synucleinopathies are diseases characterized by the aggregation of α-synuclein (α-syn), which forms fibrils through misfolding and accumulates in a prion-like manner.
Juan Huang, Xingxing Yuan, Lin Chen, Binbin Hu, Hui Wang, Ye Wang, Wei Huang   +6 more
doaj   +3 more sources

Real-time Quaking-induced Conversion Assay for the Diagnosis of Sporadic Creutzfeldt-Jakob Disease in a Living Patient [PDF]

Infectious Diseases: Research and Treatment, 2019
Creutzfeldt-Jakob disease (CJD) is the most common prion disease in humans with an incidence of one case per million inhabitants worldwide. The sporadic form of CJD (sCJD) is spontaneous and accounts for 85% of cases. Its symptoms include rapidly progressive dementia, ataxic gait, personality changes, myoclonus, coma, and eventually death.
Vikas Reddy, Neesha Settipalle
exaly   +5 more sources

Practical Real-Time Quaking-Induced Conversion for Detecting Classical Bovine Spongiform Encephalopathy and Classical and Atypical Scrapie Prions [PDF]

Pathogens
Real-time quaking-induced conversion (RT-QuIC) is highly sensitive for prion detection; however, inhibitory factors present in tissue homogenates readily interfere with the assay.
Akio Suzuki, Kazuhei Sawada, Taku Nakashima, Toyotaka Sato, Kohtaro Miyazawa, Yuichi Matsuura, Keigo Ikeda, Yoshifumi Iwamaru, Motohiro Horiuchi   +8 more
doaj   +2 more sources

Progressive multifocal diffusion-weighted imaging hyperintensities in sporadic Creutzfeldt–Jakob disease with positive cerebrospinal fluid real-time quaking-induced conversion: a case report [PDF]

Journal of Medical Case Reports
Background Creutzfeldt–Jakob disease is a rare and invariably fatal neurodegenerative disorder classified among the prion diseases. Primarily affecting the brain, this condition precipitates neuronal cell demise, culminating in swiftly advancing dementia
Puyu Li, Wenjing Wang, Lingzhi Li, Keding Cheng, Ying Wang, Yuanyuan Li, Wei Xu   +6 more
doaj   +2 more sources

Early diagnosis of a case of Heidenhain variant of Creutzfeld-Jakob disease by cerebrospinal fluid real-time quaking-induced conversion test [PDF]

Prion
Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare, rapidly progressive, and fatal neurodegenerative disorder caused by the accumulation of abnormal prion proteins in the central nervous system. The Heidenhain variant (HvCJD), a rare subtype of sCJD, is
Lin Li, Rong Yan, Jiawei Wang, Yanjun Guo   +3 more
doaj   +2 more sources