α-Synuclein real-time quaking-induced conversion in the cerebrospinal fluid of uncertain cases of parkinsonism. [PDF]
Ann Neurol, 2019 A reliable biomarker is needed for accurate and early differentiation between Parkinson disease and the various forms of atypical parkinsonism. We used a novel real‐time quaking‐induced conversion (RT‐QuIC) assay to detect α‐synuclein (α‐syn) aggregates ...
van Rumund A +5 more
europepmc +8 more sources
Application of real-time quaking-induced conversion in Creutzfeldt-Jakob disease surveillance. [PDF]
J Neurol, 2023 Background Evaluation of the application of CSF real-time quaking-induced conversion in Creutzfeldt–Jakob disease surveillance to investigate test accuracy, influencing factors, and associations with disease incidence.
Hermann P +10 more
europepmc +6 more sources
Evaluation of Real-Time Quaking-Induced Conversion, ELISA, and Immunohistochemistry for Chronic Wasting Disease Diagnosis [PDF]
Frontiers in Veterinary Science, 2022 Chronic wasting disease (CWD) is a transmissible prion disorder, primarily affecting free-ranging and captive cervids in North America (United States and Canada), South Korea, and Europe (Finland, Norway, and Sweden).
Carine L. Holz +6 more
doaj +5 more sources
Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion. [PDF]
Biomed Res Int, 2017 Prions composed of pathogenic scrapie prion protein (PrPSc) are infectious pathogens that cause progressive neurological conditions known as prion diseases or transmissible spongiform encephalopathies.
Kang HE +4 more
europepmc +5 more sources
Development of α-Synuclein Real-Time Quaking-Induced Conversion as a Diagnostic Method for α-Synucleinopathies [PDF]
Frontiers in Aging Neuroscience, 2021 Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy are characterized by aggregation of abnormal α-synuclein (α-syn) and collectively referred to as α-synucleinopathy. Because these diseases have different prognoses and treatments,
Takehiro Nakagaki +2 more
doaj +5 more sources
Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan. [PDF]
J Vet Med Sci, 2021 There has been no report on Chronic wasting disease (CWD) cases in Japan to date; however, there is concern about the geographic spread of CWD. To clarify the CWD status in Japan, we conducted CWD monitoring using real-time quaking-induced conversion (RT-
Suzuki A +6 more
europepmc +6 more sources
PAD-Beads enrichment enhances detection of PrPSc using real-time quaking-induced conversion [PDF]
BMC Research Notes, 2019 Objective Scrapie is a transmissible spongiform encephalopathy (TSE) that naturally occurs in sheep and goats. This fatal neurodegenerative disease results from misfolding of the normal cellular prion protein (PrPC) to a pathogenic prion protein form ...
Soyoun Hwang +2 more
doaj +5 more sources
Seeding activity of human superoxide dismutase 1 aggregates in familial and sporadic amyotrophic lateral sclerosis postmortem neural tissues by real-time quaking-induced conversion. [PDF]
Acta NeuropatholAmyotrophic lateral sclerosis (ALS) is a rapidly progressive neurodegenerative disease with average lifespan of 2-5 years after diagnosis. The identification of novel prognostic and pharmacodynamic biomarkers are needed to facilitate therapeutic ...
Mielke JK +10 more
europepmc +3 more sources
α-Synuclein real-time quaking-induced conversion in the submandibular glands of Parkinson's disease patients. [PDF]
Mov Disord, 2020 AbstractBackgroundIdentification of a peripheral biomarker is a major roadblock in the diagnosis of PD. Immunohistological identification of p‐serine 129 α‐synuclein in the submandibular gland tissues of PD patients has been recently reported.ObjectiveWe report on a proof‐of‐principle study for using an ultra‐sensitive and specific, real‐time quaking ...
Manne S +6 more
europepmc +5 more sources
Evaluation of Winter Ticks (Dermacentor albipictus) Collected from North American Elk (Cervus canadensis) in an Area of Chronic Wasting Disease Endemicity for Evidence of PrP CWD Amplification Using Real-Time Quaking-Induced Conversion Assay [PDF]
mSphere, 2021 This study reports the first finding of detectable levels of prions linked to chronic wasting disease in a tick collected from a clinically infected elk.
N. J. Haley +4 more
doaj +2 more sources