Results 21 to 30 of about 10,380 (154)

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. [PDF]

Nature Medicine, 2011
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions.
Yamaguchi, Naohiro, Nakagaki, Takehiro, Tetsuyuki Kitamoto, Yamada, Masahito, Masahito Yamada, Sano, Kazunori, Hitoki Yamanaka, Ryuichiro Atarashi, Fuse, Takayuki, Yamanaka, Hitoki, Mizusawa, Hidehiro, Nishida, Noriyuki, Naohiro Yamaguchi, Ishibashi, Daisuke, Atarashi, Ryuichiro, Shirabe, Susumu, Susumu Shirabe, Amelia McGlade, Kitamoto, Tetsuyuki, Kazunori Sano, Takayuki Fuse, Genevieve Klug, Steven J Collins, Matsubara, Takehiro, Hidehiro Mizusawa, Takehiro Matsubara, Satoh, Katsuya, Daisuke Ishibashi, Collins, Steven J, Katsuya Satoh, Noriyuki Nishida, Takehiro Nakagaki, Klug, Genevieve, McGlade, Amelia   +33 more
core   +3 more sources

Assessment of Real-Time Quaking-Induced Conversion (RT-QuIC) Assay, Immunohistochemistry and ELISA for Detection of Chronic Wasting Disease under Field Conditions in White-Tailed Deer: A Bayesian Approach [PDF]

Pathogens, 2022
Chronic wasting disease (CWD) is a transmissible prion disease of the cervidae family. ELISA and IHC tests performed postmortem on the medial retropharyngeal lymph nodes (RPLN) or obex are considered diagnostic gold standards for prion detection. However,
Catalina Picasso-Risso, Marc D. Schwabenlander, Gage Rowden, Michelle Carstensen, Jason C. Bartz, Peter A. Larsen, Tiffany M. Wolf   +6 more
doaj   +2 more sources

Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies

Experimental Gerontology
synucleinopathies are diseases characterized by the aggregation of α-synuclein (α-syn), which forms fibrils through misfolding and accumulates in a prion-like manner.
Juan Huang, Xingxing Yuan, Lin Chen, Binbin Hu, Hui Wang, Ye Wang, Wei Huang   +6 more
doaj   +3 more sources

Dermal Real‐Time Quaking‐Induced Conversion Is a Sensitive Marker to Confirm Isolated Rapid Eye Movement Sleep Behavior Disorder as an Early α‐Synucleinopathy

Movement Disorders, 2023
:Background:Skin biopsy is apotential tool for the premortem confirmation of anα-synucleinopathy.Objective:The aim was to assess the aggregationassay real-time quaking-induced conversion (RT-QuIC)of skin biopsy lysates to confirm isolated rapid ...
Berg, Daniela, Janzen, Annette, Salabasidou, Elena, Schaeffer, Eva, Oertel, Wolfgang H., Seger, Aline, Sommerauer, Michael, Knacke, Henrike, Sommer, Claudia, Barbe, Michael, Kohl, Antonia, Volkmann, Jens, Sittig, Elisabeth, Doppler, Kathrin, Schmitt, Daniela, Schreglmann, Sebastian R., Rößle, Jonas, Kuzkina, Anastasia, Panzer, Celine   +18 more
core   +2 more sources

Practical Real-Time Quaking-Induced Conversion for Detecting Classical Bovine Spongiform Encephalopathy and Classical and Atypical Scrapie Prions [PDF]

Pathogens
Real-time quaking-induced conversion (RT-QuIC) is highly sensitive for prion detection; however, inhibitory factors present in tissue homogenates readily interfere with the assay.
Akio Suzuki, Kazuhei Sawada, Taku Nakashima, Toyotaka Sato, Kohtaro Miyazawa, Yuichi Matsuura, Keigo Ikeda, Yoshifumi Iwamaru, Motohiro Horiuchi   +8 more
doaj   +2 more sources

Quantitative real-time quaking-induced conversion allows monitoring of disease-modifying therapy in the urine of prion-infected mice.

Journal of Neuropathology and Experimental Neurology, 2015
Prion diseases are fatal neurodegenerative diseases characterized by accumulation of the pathogenic prion protein PrP in the brain. We established quantitative real-time quaking-induced conversion for the measurement of minute amounts of PrP in body ...
Wagner, Jens, Ryazanov, Sergey, Shi, Song, Mitteregger-Kretzschmar, G., Wagner, J., Leonov, Andrei, Ryazanov, S., Giese, Armin, Mitteregger-Kretzschmar, Gerda, Griesinger, C. ; https://orcid.org/, Shi, S., Leonov, A., Giese, A., Griesinger, Christian   +13 more
core   +2 more sources

Diagnostic Value of the CSF α-Synuclein Real-Time Quaking-Induced Conversion Assay at the Prodromal MCI Stage of Dementia With Lewy Bodies. [PDF]

Neurology, 2021
OBJECTIVE: To investigate whether the CSF α-synuclein (α-syn) real-time quaking-induced conversion (RT-QuIC) assay accurately identifies patients with mild cognitive impairment (MCI) due to probable Lewy body (LB) disease.
Rossi M, Baiardi S, Teunissen CE, Quadalti C, van de Beek M, Mammana A, Stanzani-Maserati M, Van der Flier WM, Sambati L, Zenesini C, Caughey B, Capellari S, Lemstra AW, Parchi P.   +13 more
europepmc   +3 more sources

Real time quaking-induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt-Jakob disease [PDF]

Annals of Neurology, 2012
Objective:Current cerebrospinal fluid (CSF) tests for sporadic Creutzfeldt–Jakob disease (sCJD) are based on the detection of surrogate markers of neuronal damage such as CSF 14-3-3, which are not specific for sCJD.
M. W. Head, Orrú, Christina D, A. J. E. Green, L. I. McGuire, Will, Robert G, A. H. Peden, Peden, Alexander H; id_orcid, Mallinson, Gary, Knight, Richard S G, Caughey, Byron, Andrews, Mary, M. Andrews, R. S. G. Knight, B. Caughey, Head, Mark W, Wilham, Jason M, ORRU', CHRISTINA DORIANA, Appleford, Nigel E, Green, Alison J E, G. Mallinson, R. G. Will, N. E. Appleford, McGuire, Lynne I, J. M. Wilham   +23 more
core   +4 more sources

Progressive multifocal diffusion-weighted imaging hyperintensities in sporadic Creutzfeldt–Jakob disease with positive cerebrospinal fluid real-time quaking-induced conversion: a case report [PDF]

Journal of Medical Case Reports
Background Creutzfeldt–Jakob disease is a rare and invariably fatal neurodegenerative disorder classified among the prion diseases. Primarily affecting the brain, this condition precipitates neuronal cell demise, culminating in swiftly advancing dementia
Puyu Li, Wenjing Wang, Lingzhi Li, Keding Cheng, Ying Wang, Yuanyuan Li, Wei Xu   +6 more
doaj   +2 more sources

Early diagnosis of a case of Heidenhain variant of Creutzfeld-Jakob disease by cerebrospinal fluid real-time quaking-induced conversion test [PDF]

Prion
Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare, rapidly progressive, and fatal neurodegenerative disorder caused by the accumulation of abnormal prion proteins in the central nervous system. The Heidenhain variant (HvCJD), a rare subtype of sCJD, is
Lin Li, Rong Yan, Jiawei Wang, Yanjun Guo   +3 more
doaj   +2 more sources