Results 21 to 30 of about 20,909,028 (154)

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. [PDF]

Nature Medicine, 2011
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions.
Yamaguchi, Naohiro, Nakagaki, Takehiro, Tetsuyuki Kitamoto, Yamada, Masahito, Masahito Yamada, Sano, Kazunori, Hitoki Yamanaka, Ryuichiro Atarashi, Fuse, Takayuki, Yamanaka, Hitoki, Mizusawa, Hidehiro, Nishida, Noriyuki, Naohiro Yamaguchi, Ishibashi, Daisuke, Atarashi, Ryuichiro, Shirabe, Susumu, Susumu Shirabe, Amelia McGlade, Kitamoto, Tetsuyuki, Kazunori Sano, Takayuki Fuse, Genevieve Klug, Steven J Collins, Matsubara, Takehiro, Hidehiro Mizusawa, Takehiro Matsubara, Satoh, Katsuya, Daisuke Ishibashi, Collins, Steven J, Katsuya Satoh, Noriyuki Nishida, Takehiro Nakagaki, Klug, Genevieve, McGlade, Amelia   +33 more
core   +3 more sources

Real-time quaking-induced conversion assays for prions: Applying a sensitive but imperfect test in clinical practice. [PDF]

Eur J Neurol, 2023
AbstractBackground and purposeReal‐time quaking‐induced conversion (RT‐QuIC) assays offer a sensitive and specific means for detection of prions, although false negative results are recognized in clinical practice. We profile the clinical, laboratory, and pathologic features associated with false negative RT‐QuIC assays and extend these to frame the ...
Jones SM, Lazar EB, Porter AL, Prusinski CC, Brier MR, Bucelli RC, Day GS.   +6 more
europepmc   +4 more sources

Real time quaking-induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt-Jakob disease [PDF]

Annals of Neurology, 2012
Objective:Current cerebrospinal fluid (CSF) tests for sporadic Creutzfeldt–Jakob disease (sCJD) are based on the detection of surrogate markers of neuronal damage such as CSF 14-3-3, which are not specific for sCJD.
M. W. Head, Orrú, Christina D, A. J. E. Green, L. I. McGuire, Will, Robert G, A. H. Peden, Peden, Alexander H; id_orcid, Mallinson, Gary, Knight, Richard S G, Caughey, Byron, Andrews, Mary, M. Andrews, R. S. G. Knight, B. Caughey, Head, Mark W, Wilham, Jason M, ORRU', CHRISTINA DORIANA, Appleford, Nigel E, Green, Alison J E, G. Mallinson, R. G. Will, N. E. Appleford, McGuire, Lynne I, J. M. Wilham   +23 more
core   +5 more sources

Pathological α-synuclein detected by real-time quaking-induced conversion in synucleinopathies

Experimental Gerontology
synucleinopathies are diseases characterized by the aggregation of α-synuclein (α-syn), which forms fibrils through misfolding and accumulates in a prion-like manner.
Juan Huang, Xingxing Yuan, Lin Chen, Binbin Hu, Hui Wang, Ye Wang, Wei Huang   +6 more
doaj   +3 more sources

Real-time quaking-induced conversion [PDF]

Prion, 2011
We recently developed a new in vitro amplification technology, designated "real-time quaking-induced conversion (RT-QUIC)", for detection of the abnormal form of prion protein (PrPSc) in easily accessible specimens such as cerebrospinal fluid (CSF).
Ryuichiro Atarashi, Kazunori Sano, Katsuya Satoh, Noriyuki Nishida   +3 more
core   +3 more sources

Practical Real-Time Quaking-Induced Conversion for Detecting Classical Bovine Spongiform Encephalopathy and Classical and Atypical Scrapie Prions [PDF]

Pathogens
Real-time quaking-induced conversion (RT-QuIC) is highly sensitive for prion detection; however, inhibitory factors present in tissue homogenates readily interfere with the assay.
Akio Suzuki, Kazuhei Sawada, Taku Nakashima, Toyotaka Sato, Kohtaro Miyazawa, Yuichi Matsuura, Keigo Ikeda, Yoshifumi Iwamaru, Motohiro Horiuchi   +8 more
doaj   +2 more sources

Progressive multifocal diffusion-weighted imaging hyperintensities in sporadic Creutzfeldt–Jakob disease with positive cerebrospinal fluid real-time quaking-induced conversion: a case report [PDF]

Journal of Medical Case Reports
Background Creutzfeldt–Jakob disease is a rare and invariably fatal neurodegenerative disorder classified among the prion diseases. Primarily affecting the brain, this condition precipitates neuronal cell demise, culminating in swiftly advancing dementia
Puyu Li, Wenjing Wang, Lingzhi Li, Keding Cheng, Ying Wang, Yuanyuan Li, Wei Xu   +6 more
doaj   +2 more sources

Early diagnosis of a case of Heidenhain variant of Creutzfeld-Jakob disease by cerebrospinal fluid real-time quaking-induced conversion test [PDF]

Prion
Sporadic Creutzfeldt-Jakob disease (sCJD) is a rare, rapidly progressive, and fatal neurodegenerative disorder caused by the accumulation of abnormal prion proteins in the central nervous system. The Heidenhain variant (HvCJD), a rare subtype of sCJD, is
Lin Li, Rong Yan, Jiawei Wang, Yanjun Guo   +3 more
doaj   +2 more sources

quicR: An R library for streamlined data handling of real-time quaking induced conversion assays

SoftwareX
Real-time quaking induced conversion (RT-QuIC) has become a valuable diagnostic tool for protein misfolding disorders such as Creutzfeldt–Jakob disease and Parkinson’s disease.
Gage R. Rowden, Peter A. Larsen
doaj   +2 more sources

Early and Non-Invasive Detection of Chronic Wasting Disease Prions in Elk Feces by Real-Time Quaking Induced Conversion. [PDF]

PLoS ONE, 2016
Chronic wasting disease (CWD) is a fatal prion disease of wild and captive cervids in North America. Prions are infectious agents composed of a misfolded version of a host-encoded protein, termed PrPSc.
Yo Ching Cheng, Samia Hannaoui, Theodore R John, Sandor Dudas, Stefanie Czub, Sabine Gilch   +5 more
doaj   +2 more sources