Results 1 to 10 of about 2,250 (151)

Detection of CWD in cervids by RT-QuIC assay of third eyelids.

PLoS ONE, 2019
The diagnosis of chronic wasting disease (CWD) relies on demonstration of the disease-associated misfolded CWD prion protein (PrPCWD) in brain or retropharyngeal lymph node tissue by immunodetection methods, e.g. ELISA and immunohistochemistry (IHC). The
Sarah K Cooper, Clare E Hoover, Davin M Henderson, Nicholas J Haley, Candace K Mathiason, Edward A Hoover   +5 more
doaj   +3 more sources

α‐synuclein RT‐QuIC in cerebrospinal fluid of LRRK2‐linked Parkinson's disease [PDF]

Annals of Clinical and Translational Neurology, 2019
Background Leucine‐rich kinase 2 (LRRK2)‐linked Parkinson's disease (PD) is clinically indistinguishable from idiopathic PD (IPD). A pleiotropic neuropathology has been recognized but the majority of studies in LRRK2 p.G2019S patients reveal Lewy‐type ...
Alicia Garrido, Graham Fairfoul, Eduardo S. Tolosa, Maria José Martí, Alison Green, the Barcelona LRRK2 Study Group   +5 more
doaj   +5 more sources

A noninvasive test for human prion disease using hair roots and scalp

Scientific Reports
Invasive tests like cerebrospinal fluid (CSF) examination are highly effective for diagnosing human prion disease (HPD). Real-time quaking-induced conversion (RT-QuIC) CSF assay demonstrates 80–90% sensitivity for HPD diagnosis.
Thi-Thu-Trang Dong, Hiroyuki Honda, Akio Akagi, Yasushi Iwasaki, Hitaru Kishida, Tadashi Tsukamoto, Kensaku Kasuga, Hirotsugu Takashima, Tatsuhiro Terada, Kensuke Ikenaka, Takeshi Ikeuchi, Tsuyoshi Mori, Hideki Mochizuki, Kenjiro Ono, Yoshihisa Takiyama, Tsuyoshi Hamaguchi, Hiroyuki Murota, Nobuo Sanjo, Takeshi Fujimoto, Michio Kitayama, Koji Fujita, Motohiro Yukitake, Shinsuke Fujioka, Noriyuki Nishida, Yoshio Tsuboi, Tetsuyuki Kitamoto, Masaki Takao, Masahito Yamada, Hidehiro Mizusawa, Katsuya Satoh   +29 more
doaj   +1 more source

Defining the Protein Seeds of Neurodegeneration using Real-Time Quaking-Induced Conversion Assays

Biomolecules, 2020
Neurodegenerative diseases are characterized by the accumulation of disease-related misfolded proteins. It is now widely understood that the characteristic self-amplifying (i.e., seeding) capacity once only attributed to the prions of transmissible ...
Matteo Manca, Allison Kraus
doaj   +1 more source

Seeding amplification assay with Universal Control Fluid: Standardized detection of α-synucleinopathies.

PLoS ONE
Seeding amplification assays, specifically the Real-Time Quaking-Induced Conversion method (RT-QuIC), have shown great diagnostic potential for α-synucleinopathies.
Remarh Bsoul, Anja H Simonsen, Kristian S Frederiksen, Kirsten Svenstrup, Sara Bech, Lisette Salvesen, Anne-Mette Hejl, Marcello Rossi, Piero Parchi, Eva L Lund, Aušrinė Areškevičiūtė   +10 more
doaj   +1 more source

Epitope mapping of the protease resistant products of RT-QuIC does not allow the discrimination of sCJD subtypes.

PLoS ONE, 2019
Sporadic Creutzfeldt-Jakob disease (sCJD) is a transmissible, rapidly progressive and fatal neurodegenerative disease. The transmissible agent linked to sCJD is composed of the misfolded form of the host-encoded prion protein.
Gabriele Piconi, Alexander H Peden, Marcelo A Barria, Alison J E Green   +3 more
doaj   +1 more source

Rapid and Sensitive RT-QuIC Detection of Human Creutzfeldt-Jakob Disease Using Cerebrospinal Fluid

mBio, 2015
Fast, definitive diagnosis of Creutzfeldt-Jakob disease (CJD) is important in assessing patient care options and transmission risks. Real-time quaking-induced conversion (RT-QuIC) assays of cerebrospinal fluid (CSF) and nasal-brushing specimens are ...
Christina D. Orrú, Bradley R. Groveman, Andrew G. Hughson, Gianluigi Zanusso, Michael B. Coulthart, Byron Caughey   +5 more
doaj   +1 more source

Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. [PDF]

PLoS Pathogens, 2010
A major problem for the effective diagnosis and management of prion diseases is the lack of rapid high-throughput assays to measure low levels of prions. Such measurements have typically required prolonged bioassays in animals.
Jason M Wilham, Christina D Orrú, Richard A Bessen, Ryuichiro Atarashi, Kazunori Sano, Brent Race, Kimberly D Meade-White, Lara M Taubner, Andrew Timmes, Byron Caughey   +9 more
doaj   +1 more source

Bank Vole Prion Protein As an Apparently Universal Substrate for RT-QuIC-Based Detection and Discrimination of Prion Strains.

PLoS Pathogens, 2015
Prions propagate as multiple strains in a wide variety of mammalian species. The detection of all such strains by a single ultrasensitive assay such as Real Time Quaking-induced Conversion (RT-QuIC) would facilitate prion disease diagnosis, surveillance ...
Christina D Orrú, Bradley R Groveman, Lynne D Raymond, Andrew G Hughson, Romolo Nonno, Wenquan Zou, Bernardino Ghetti, Pierluigi Gambetti, Byron Caughey   +8 more
doaj   +1 more source

A single ultrasensitive assay for detection and discrimination of tau aggregates of Alzheimer and Pick diseases

Acta Neuropathologica Communications, 2020
Multiple neurodegenerative diseases are characterized by aggregation of tau molecules. Adult humans express six isoforms of tau that contain either 3 or 4 microtubule binding repeats (3R or 4R tau).
Michael A. Metrick, Natália do Carmo Ferreira, Eri Saijo, Allison Kraus, Kathy Newell, Gianluigi Zanusso, Michele Vendruscolo, Bernardino Ghetti, Byron Caughey   +8 more
doaj   +1 more source