Standardization of Data Analysis for RT-QuIC-Based Detection of Chronic Wasting Disease [PDF]
Pathogens, 2023 Chronic wasting disease (CWD) is a disease affecting cervids and is caused by prions accumulating as pathogenic fibrils in lymphoid tissue and the central nervous system.
Gage Rowden +2 more
exaly +7 more sources
Misfolded alpha-synuclein detection by RT-QuIC in dementia with lewy bodies: a systematic review and meta-analysis [PDF]
Frontiers in Molecular Biosciences, 2023 Introduction: Dementia with Lewy Bodies (DLB) is the second most common cause of neurodegenerative dementia after Alzheimer’s disease (AD), but the field is still lacking a specific biomarker for its core pathology: alpha synuclein (α-syn).
Carmen Peña-Bautista +8 more
exaly +5 more sources
Development of a sensitive real-time quaking-induced conversion (RT-QuIC) assay for application in prion-infected blood. [PDF]
PLoS ONE, 2023 Efforts to prevent human-to-human transmission of variant Creutzfeldt-Jakob disease (vCJD) by contaminated blood would be aided by the development of a sensitive diagnostic test that could be routinely used to screen blood donations.
Charlotte M Thomas +7 more
doaj +7 more sources
RT-QuIC optimization for prion detection in soils [PDF]
MethodsXChronic wasting disease (CWD) is a transmissible neurodegenerative prion disorder affecting cervid species, such as deer, characterized by neurological decline and death.
Madeline K. Grunklee +6 more
doaj +4 more sources
Performance of αSynuclein RT-QuIC in relation to neuropathological staging of Lewy body disease [PDF]
Acta Neuropathologica Communications, 2022 Currently, there is a need for diagnostic markers in Lewy body disorders (LBD). α-synuclein (αSyn) RT-QuIC has emerged as a promising assay to detect misfolded αSyn in clinically or neuropathologically established patients with various synucleinopathies.
Sara Hall +9 more
doaj +6 more sources
The Latest Research on RT-QuIC Assays—A Literature Review [PDF]
Pathogens, 2021 The misfolding of proteins such as the prion protein, α-synuclein, and tau represents a key initiating event for pathogenesis of most common neurodegenerative disorders, and its presence correlates with infectivity.
Thi-Thu-Trang Dong, Katsuya Satoh
exaly +7 more sources
Diagnostic value of skin RT-QuIC in Parkinson’s disease: a two-laboratory study [PDF]
npj Parkinson's Disease, 2021 Skin α-synuclein deposition is considered a potential biomarker for Parkinson’s disease (PD). Real-time quaking-induced conversion (RT-QuIC) is a novel, ultrasensitive, and efficient seeding assay that enables the detection of minute amounts of α ...
Anastasia Kuzkina +12 more
doaj +7 more sources
Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson’s disease and dementia with Lewy bodies [PDF]
Acta Neuropathologica Communications, 2021 Definitive diagnosis of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) relies on postmortem finding of disease-associated alpha-synuclein (αSynD) as misfolded protein aggregates in the central nervous system (CNS). The recent development of
Connor Bargar +14 more
doaj +3 more sources
Detection of skin α-synuclein using RT-QuIC as a diagnostic biomarker for Parkinson’s disease in the Chinese population [PDF]
European Journal of Medical ResearchBackground Several studies have indicated that skin holds promise as a potential sample for detecting pathological α-Syn and serving as a diagnostic biomarker for α-synucleinopathies. Despite reports in Chinese PD patients, comprehensive research on skin
Jiaqi Li +10 more
doaj +3 more sources
Validation and Application of Skin RT-QuIC to Patients in China with Probable CJD [PDF]
Pathogens, 2021 The definite diagnosis of human sporadic Creutzfeldt–Jakob disease (sCJD) largely depends on postmortem neuropathology and PrPSc detection in the brain.
Cao Chen, Qi Shi
exaly +4 more sources