RT-QuIC optimization for prion detection in soils [PDF]
MethodsXChronic wasting disease (CWD) is a transmissible neurodegenerative prion disorder affecting cervid species, such as deer, characterized by neurological decline and death.
Madeline K. Grunklee +6 more
doaj +4 more sources
Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that causes a fatal neurodegenerative disease in cervids. Cases of CWD are rapidly increasing in North America among wild and farmed cervid populations, and potential for ...
Soyoun Hwang +6 more
doaj +5 more sources
α‐synuclein RT‐QuIC in cerebrospinal fluid of LRRK2‐linked Parkinson's disease [PDF]
Annals of Clinical and Translational Neurology, 2019 Background Leucine‐rich kinase 2 (LRRK2)‐linked Parkinson's disease (PD) is clinically indistinguishable from idiopathic PD (IPD). A pleiotropic neuropathology has been recognized but the majority of studies in LRRK2 p.G2019S patients reveal Lewy‐type ...
Alicia Garrido +5 more
doaj +7 more sources
Ring trial of 2nd generation RT‐QuIC diagnostic tests for sporadic CJD [PDF]
Annals of Clinical and Translational Neurology, 2020 Objective Real‐time quaking‐induced conversion (RT‐QuIC) assays detect prion‐seeding activity in a variety of human biospecimens, including cerebrospinal fluid and olfactory mucosa swabs.
Christina D. Orrú +21 more
doaj +7 more sources
Diagnosis of prion diseases by RT-QuIC results in improved surveillance [PDF]
Neurology, 2020 Objective: We present the National Prion Disease Pathology Surveillance Center's (NPDPSC) experience using cerebrospinal fluid (CSF) real time quaking induced conversion (RT-QuIC) as a diagnostic test, examine factors associated with false negative RT ...
Appleby, Brian
core +3 more sources
Optimization of the RT-QuIC in prion disease diagnostic [PDF]
, 2022 In the present work, we were interested in applying different recombinant prion proteins (rec PrP) as substrates in the real-time quaking-induced conversion (RT-QuIC) assay to improve the diagnostic accuracy in different prion diseases.
da Silva Correia, Susana Margarida
core +3 more sources
Detection of CWD in cervids by RT-QuIC assay of third eyelids.
PLoS ONE, 2019 The diagnosis of chronic wasting disease (CWD) relies on demonstration of the disease-associated misfolded CWD prion protein (PrPCWD) in brain or retropharyngeal lymph node tissue by immunodetection methods, e.g. ELISA and immunohistochemistry (IHC). The
Sarah K Cooper +5 more
doaj +3 more sources
Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid [PDF]
Acta Neuropathologica CommunicationsThe real-time quaking-induced conversion (RT-QuIC) assay has revolutionized prion disease diagnosis by detecting amyloidogenic PrP conformers in different tissue types and body fluids.
Susana Da Silva Correia +12 more
doaj +2 more sources
Stability and Reproducibility Underscore Utility of RT-QuIC for Diagnosis of Creutzfeldt-Jakob Disease [PDF]
Molecular Neurobiology, 2015 Real-time quaking-induced conversion (RT-QuIC) allows the amplification of miniscule amounts of scrapie prion protein (PrP(Sc)). Recent studies applied the RT-QuIC methodology to cerebrospinal fluid (CSF) for diagnosing human prion diseases.
Steven Collins +35 more
core +7 more sources
Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC [PDF]
BMC Research Notes, 2018 Objective Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases, often referred as prion diseases. TSEs result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that ...
Soyoun Hwang +3 more
doaj +3 more sources