Results 11 to 20 of about 616,892 (193)

A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens. [PDF]

NPJ Biosens
Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and other synucleinopathies are characterized by the accumulation of abnormal, self-propagating aggregates of α-synuclein. RT-QuIC or seed amplification assays are currently showing unprecedented
Parveen S, Alam P, Orrù CD, Vascellari S, Hughson AG, Zou WQ, Beach TG, Serrano GE, Goldstein DS, Ghetti B, Cossu G, Pisano G, Pinna B, Caughey B.   +13 more
europepmc   +7 more sources

Misfolded α-Synuclein Assessment in the Skin and CSF by RT-QuIC in Isolated REM Sleep Behavior Disorder. [PDF]

Neurology, 2023
Background and Objectives Real-time quaking-induced conversion (RT-QuIC) assay detects misfolded α-synuclein (AS) in the skin and CSF of patients with the synucleinopathies Parkinson disease and dementia with Lewy bodies.
Iranzo A, Mammana A, Muñoz-Lopetegi A, Dellavalle S, Mayà G, Rossi M, Serradell M, Baiardi S, Arqueros A, Quadalti C, Perissinotti A, Ruggeri E, Cano JS, Gaig C, Parchi P.   +14 more
europepmc   +5 more sources

Factors That Improve RT-QuIC Detection of Prion Seeding Activity [PDF]

Viruses, 2016
Rapid and sensitive detection of prions is important in managing prion diseases. The real-time quaking-induced conversion (RT-QuIC) assay for prion seeding activity has been applied to many prion diseases and provides for specific antemortem diagnostic ...
Bradley Groveman, Katrina J Campbell, Allison Kraus   +2 more
exaly   +5 more sources

Prion Seeding Activity in Plant Tissues Detected by RT-QuIC. [PDF]

Pathogens
Prion diseases such as scrapie, bovine spongiform encephalopathy (BSE), and chronic wasting disease (CWD) affect domesticated and wild herbivorous mammals. Animals afflicted with CWD, the transmissible spongiform encephalopathy of cervids (deer, elk, and
Burgener K, Lichtenberg SS, Walsh DP, Inzalaco HN, Lomax A, Pedersen JA.   +5 more
europepmc   +5 more sources

RT-QuIC: a highly promising diagnostic method for neurodegenerative diseases-advantages and limitations. [PDF]

Front Neurol
Although it has been more than 200 years since Parkinson’s disease was described, we have not established biomarkers for its definitive diagnosis yet.
Koníčková D, Hraboš D, Menšíková K, Tučková L, Kaleta M, Strnad M, Colosimo C, Kaňovský P.   +7 more
europepmc   +5 more sources

Enhanced quantitation of pathological α-synuclein in patient biospecimens by RT-QuIC seed amplification assays. [PDF]

PLoS Pathog
Disease associated pathological aggregates of alpha-synuclein (αSynD) exhibit prion-like spreading in synucleinopathies such as Parkinson’s disease (PD) and dementia with Lewy bodies (DLB).
Srivastava A, Wang Q, Orrù CD, Fernandez M, Compta Y, Ghetti B, Zanusso G, Zou WQ, Caughey B, Beauchemin CAA.   +9 more
europepmc   +5 more sources

Quantification and diagnostic relevance of blood and heme-mediated inhibition of prion detection by RT-QuIC [PDF]

Journal of Clinical Microbiology
Prion diseases are characterized by misfolding of prion protein (PrP) from correctly folded PrPC to a disease-associated form, PrPD. Real-time quaking-induced conversion (RT-QuIC) detects prions by “seeding” reaction mixtures, which contain recombinant ...
Robert B. Piel, David A. Schneider
doaj   +3 more sources

Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that causes a fatal neurodegenerative disease in cervids. Cases of CWD are rapidly increasing in North America among wild and farmed cervid populations, and potential for ...
Soyoun Hwang, Danielle Beckley, Danielle Beckley, Konstantin P. Alekseev, Konstantin P. Alekseev, Konstantin P. Alekseev, Eric M. Nicholson   +6 more
doaj   +5 more sources

α‐synuclein RT‐QuIC in cerebrospinal fluid of LRRK2‐linked Parkinson's disease [PDF]

Annals of Clinical and Translational Neurology, 2019
Background Leucine‐rich kinase 2 (LRRK2)‐linked Parkinson's disease (PD) is clinically indistinguishable from idiopathic PD (IPD). A pleiotropic neuropathology has been recognized but the majority of studies in LRRK2 p.G2019S patients reveal Lewy‐type ...
Alicia Garrido, Graham Fairfoul, Eduardo S. Tolosa, Maria José Martí, Alison Green, the Barcelona LRRK2 Study Group   +5 more
doaj   +7 more sources

Ring trial of 2nd generation RT‐QuIC diagnostic tests for sporadic CJD [PDF]

Annals of Clinical and Translational Neurology, 2020
Objective Real‐time quaking‐induced conversion (RT‐QuIC) assays detect prion‐seeding activity in a variety of human biospecimens, including cerebrospinal fluid and olfactory mucosa swabs.
Christina D. Orrú, Bradley R. Groveman, Aaron Foutz, Matilde Bongianni, Franco Cardone, Neil McKenzie, Audrey Culeux, Anna Poleggi, Katarina Grznarova, Daniela Perra, Michele Fiorini, Xiaoqin Liu, Anna Ladogana, Marco Sbriccoli, Andrew G. Hughson, Stéphane Haïk, Alison J. Green, Michael D. Geschwind, Maurizio Pocchiari, Jiri G. Safar, Gianluigi Zanusso, Byron Caughey   +21 more
doaj   +8 more sources