The Latest Research on RT-QuIC Assays—A Literature Review [PDF]
Pathogens, 2021 The misfolding of proteins such as the prion protein, α-synuclein, and tau represents a key initiating event for pathogenesis of most common neurodegenerative disorders, and its presence correlates with infectivity.
Katsuya Satoh +2 more
exaly +7 more sources
Validation and Application of Skin RT-QuIC to Patients in China with Probable CJD [PDF]
Pathogens, 2021 The definite diagnosis of human sporadic Creutzfeldt–Jakob disease (sCJD) largely depends on postmortem neuropathology and PrPSc detection in the brain.
Xiaoping Dong, Cao Chen, Qi Shi
exaly +7 more sources
Standardization of Data Analysis for RT-QuIC-Based Detection of Chronic Wasting Disease [PDF]
Pathogens, 2023 Chronic wasting disease (CWD) is a disease affecting cervids and is caused by prions accumulating as pathogenic fibrils in lymphoid tissue and the central nervous system.
Gage R. Rowden +5 more
doaj +4 more sources
Factors That Improve RT-QuIC Detection of Prion Seeding Activity [PDF]
Viruses, 2016 Rapid and sensitive detection of prions is important in managing prion diseases. The real-time quaking-induced conversion (RT-QuIC) assay for prion seeding activity has been applied to many prion diseases and provides for specific antemortem diagnostic ...
Matteo Manca +2 more
exaly +5 more sources
RT-QuIC optimization for prion detection in soils [PDF]
MethodsXChronic wasting disease (CWD) is a transmissible neurodegenerative prion disorder affecting cervid species, such as deer, characterized by neurological decline and death.
Madeline K. Grunklee +6 more
doaj +4 more sources
Performance of αSynuclein RT-QuIC in relation to neuropathological staging of Lewy body disease [PDF]
Acta Neuropathologica Communications, 2022 Currently, there is a need for diagnostic markers in Lewy body disorders (LBD). α-synuclein (αSyn) RT-QuIC has emerged as a promising assay to detect misfolded αSyn in clinically or neuropathologically established patients with various synucleinopathies.
Sara Hall +9 more
doaj +5 more sources
Diagnostic value of skin RT-QuIC in Parkinson’s disease: a two-laboratory study [PDF]
npj Parkinson's Disease, 2021 Skin α-synuclein deposition is considered a potential biomarker for Parkinson’s disease (PD). Real-time quaking-induced conversion (RT-QuIC) is a novel, ultrasensitive, and efficient seeding assay that enables the detection of minute amounts of α ...
Anastasia Kuzkina +12 more
doaj +5 more sources
Quantification and diagnostic relevance of blood and heme-mediated inhibition of prion detection by RT-QuIC [PDF]
Journal of Clinical MicrobiologyPrion diseases are characterized by misfolding of prion protein (PrP) from correctly folded PrPC to a disease-associated form, PrPD. Real-time quaking-induced conversion (RT-QuIC) detects prions by “seeding” reaction mixtures, which contain recombinant ...
Robert B. Piel, David A. Schneider
doaj +5 more sources
Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that causes a fatal neurodegenerative disease in cervids. Cases of CWD are rapidly increasing in North America among wild and farmed cervid populations, and potential for ...
Soyoun Hwang +6 more
doaj +5 more sources
Ring trial of 2nd generation RT‐QuIC diagnostic tests for sporadic CJD [PDF]
Annals of Clinical and Translational Neurology, 2020 Objective Real‐time quaking‐induced conversion (RT‐QuIC) assays detect prion‐seeding activity in a variety of human biospecimens, including cerebrospinal fluid and olfactory mucosa swabs.
Christina D. Orrú +21 more
doaj +7 more sources