Results 31 to 40 of about 616,892 (193)

Optimization of the RT-QuIC in prion disease diagnostic [PDF]

, 2022
In the present work, we were interested in applying different recombinant prion proteins (rec PrP) as substrates in the real-time quaking-induced conversion (RT-QuIC) assay to improve the diagnostic accuracy in different prion diseases.
da Silva Correia, Susana Margarida
core   +2 more sources

RT-QuIC and Related Assays for Detecting and Quantifying Prion-like Pathological Seeds of α-Synuclein. [PDF]

Biomolecules, 2022
Various disease-associated forms or strains of α-synuclein (αSynD) can spread and accumulate in a prion-like fashion during synucleinopathies such as Parkinson’s disease (PD), Lewy body dementia (DLB), and multiple system atrophy (MSA). This capacity for
Srivastava A, Alam P, Caughey B.
europepmc   +2 more sources

Salivary α-Synuclein RT-QuIC Correlates with Disease Severity in de novo Parkinson's Disease. [PDF]

Mov Disord, 2023
generalized and lasted for ...
Vivacqua G, Mason M, De Bartolo MI, Węgrzynowicz M, Calò L, Belvisi D, Suppa A, Fabbrini G, Berardelli A, Spillantini M.   +9 more
europepmc   +2 more sources

Bank Vole Prion Protein As an Apparently Universal Substrate for RT-QuIC-Based Detection and Discrimination of Prion Strains. [PDF]

PLoS Pathogens, 2015
Prions propagate as multiple strains in a wide variety of mammalian species. The detection of all such strains by a single ultrasensitive assay such as Real Time Quaking-induced Conversion (RT-QuIC) would facilitate prion disease diagnosis, surveillance ...
Christina D Orrú, Bradley R Groveman, Lynne D Raymond, Andrew G Hughson, Romolo Nonno, Wenquan Zou, Bernardino Ghetti, Pierluigi Gambetti, Byron Caughey   +8 more
doaj   +3 more sources

Olfactory swab sampling optimization for α-synuclein aggregate detection in patients with Parkinson’s disease [PDF]

Translational Neurodegeneration, 2022
Background In patients with Parkinson’s disease (PD), real-time quaking-induced conversion (RT-QuIC) detection of pathological α-synuclein (α-syn) in olfactory mucosa (OM) is not as accurate as in other α-synucleinopathies.
Matilde Bongianni, Mauro Catalan, Daniela Perra, Elena Fontana, Francesco Janes, Claudio Bertolotti, Luca Sacchetto, Stefano Capaldi, Matteo Tagliapietra, Paola Polverino, Valentina Tommasini, Giulia Bellavita, Elham Ataie Kachoie, Roberto Baruca, Andrea Bernardini, Mariarosaria Valente, Michele Fiorini, Erika Bronzato, Stefano Tamburin, Laura Bertolasi, Lorenzo Brozzetti, Maria Paola Cecchini, Gianluigi Gigli, Salvatore Monaco, Paolo Manganotti, Gianluigi Zanusso   +25 more
doaj   +3 more sources

Optimization of RT-QuIC Assay Duration for Screening Chronic Wasting Disease in White-Tailed Deer. [PDF]

Vet Sci
Simple Summary Chronic wasting disease (CWD) is a prion disease in cervids that inevitably leads to fatal damage to the nervous system. The spread of CWD has dramatically impacted cervid health and the economic viability of the cervid industry.
Yilmaz G, Morrill T, Pilot W, Ward C, Mitchell G, Soutyrine A, Dan H, Lin M, Guan J.   +8 more
europepmc   +2 more sources

Monomeric α-Synuclein Real-Time Induced Conversion: A New Approach to the Diagnostics of Neurodegenerative Synucleinopathies with Weak RT-QuIC Responses. [PDF]

Acta Naturae
Neurodegenerative disorders classified as synucleinopathies (Parkinson’s disease, dementia with Lewy bodies, and multiple-system atrophy) are characterized by the accumulation of aberrant α-synuclein aggregates in neurons and glial cells.
Orlova DA, Kudriaeva AA, Kolotyeva NA, Ivanova EO, Fedotova EY, Tregub PP, Salmina AB, Illarioshkin SN, Jr AAB.   +8 more
europepmc   +2 more sources

Validation of a real-time quaking-induced conversion (RT-QuIC) assay protocol to detect chronic wasting disease using rectal mucosa of naturally infected, pre-clinical white-tailed deer (Odocoileus virginianus). [PDF]

PLoS One
Chronic wasting disease (CWD) is a fatal prion disease of cervids spreading across North America. More effective mitigation efforts may require expansion of the available toolkit to include new methods that provide earlier antemortem detection, higher ...
Piel RB, Veneziano SE, Nicholson EM, Walsh DP, Lomax AD, Nichols TA, Seabury CM, Schneider DA.   +7 more
europepmc   +2 more sources

Alpha-synuclein RT-QuIC assay in gastroduodenal and skin biopsies of Parkinson disease patients. [PDF]

Ann Clin Transl Neurol
In this study, we compared the value of pathological alpha‐synuclein (αSyn) seed amplification assay (SAA) in gastric and duodenal biopsies with skin biopsies in Parkinson disease (PD) patients with different disease duration.
Emmi A, Mammana A, Sandre M, Baiardi S, Weis L, Rossi M, Magliocchetti F, Savarino E, Russo FP, Porzionato A, Carecchio M, Campagnolo M, Antonini A, Parchi P.   +13 more
europepmc   +2 more sources

Neurofilament light chain and α-synuclein RT-QuIC as differential diagnostic biomarkers in parkinsonisms and related syndromes. [PDF]

NPJ Parkinsons Dis, 2021
Neurofilament light chain (NfL) and α-synuclein oligomeric seeds (α-syn-s) are promising biomarkers for patients with parkinsonism. We assessed their performance in discriminating Parkinson disease (PD) from atypical parkinsonisms (APDs) and evaluated ...
Quadalti C, Calandra-Buonaura G, Baiardi S, Mastrangelo A, Rossi M, Zenesini C, Giannini G, Candelise N, Sambati L, Polischi B, Plazzi G, Capellari S, Cortelli P, Parchi P.   +13 more
europepmc   +2 more sources