Results 31 to 40 of about 2,266 (154)

Prion Seeding Activity in Plant Tissues Detected by RT-QuIC. [PDF]

Pathogens
Prion diseases such as scrapie, bovine spongiform encephalopathy (BSE), and chronic wasting disease (CWD) affect domesticated and wild herbivorous mammals. Animals afflicted with CWD, the transmissible spongiform encephalopathy of cervids (deer, elk, and moose), shed prions into the environment, where they may persist and remain infectious for years ...
Burgener K, Lichtenberg SS, Walsh DP, Inzalaco HN, Lomax A, Pedersen JA.   +5 more
europepmc   +4 more sources

Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson’s disease and dementia with Lewy bodies

Acta Neuropathologica Communications, 2021
Definitive diagnosis of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) relies on postmortem finding of disease-associated alpha-synuclein (αSynD) as misfolded protein aggregates in the central nervous system (CNS). The recent development of
Connor Bargar, Wen Wang, Steven A. Gunzler, Alexandra LeFevre, Zerui Wang, Alan J. Lerner, Neena Singh, Curtis Tatsuoka, Brian Appleby, Xiongwei Zhu, Rong Xu, Vahram Haroutunian, Wen-Quan Zou, Jiyan Ma, Shu G. Chen   +14 more
doaj   +1 more source

Diagnosis of prion diseases by RT-QuIC results in improved surveillance [PDF]

Neurology, 2020
To present the National Prion Disease Pathology Surveillance Center's (NPDPSC's) experience using CSF real-time quaking-induced conversion (RT-QuIC) as a diagnostic test, to examine factors associated with false-negative RT-QuIC results, and to investigate the impact of RT-QuICs on prion disease surveillance.Between May 2015 and April 2018, the NPDPSC ...
Daniel D. Rhoads, Aleksandra Wrona, Aaron Foutz, Janis Blevins, Kathleen Glisic, Marissa Person, Ryan A. Maddox, Ermias D. Belay, Lawrence B. Schonberger, Curtis Tatsuoka, Mark L. Cohen, Brian S. Appleby   +11 more
openaire   +2 more sources

THE DIAGNOSTIC UTILITY OF RT-QUIC IN SPORADIC CJD [PDF]

Journal of Neurology, Neurosurgery & Psychiatry, 2014
Sporadic CJD (sCJD) remains the commonest type of CJD. Currently, there is not a disease specific antemortem test available for the diagnosis of sCJD with postmortem remaining the definitive method of diagnosis. Current diagnostic criteria rely on clinical presentation in association with the MRI, EEG and CSF 14–3–3 protein.
Louise Davidson, Alison Green, Lynne McGuire, Robert G Will, Richard Knight   +4 more
openaire   +1 more source

Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear

Scientific Reports, 2021
Efforts to contain the spread of chronic wasting disease (CWD), a fatal, contagious prion disease of cervids, would be aided by the availability of additional diagnostic tools.
Natalia C. Ferreira, Jorge M. Charco, Jakob Plagenz, Christina D. Orru, Nathanial D. Denkers, Michael A. Metrick, Andrew G. Hughson, Karen A. Griffin, Brent Race, Edward A. Hoover, Joaquín Castilla, Tracy A. Nichols, Michael W. Miller, Byron Caughey   +13 more
doaj   +1 more source

Anti-Prion Screening for Acridine, Dextran, and Tannic Acid using Real Time-Quaking Induced Conversion: A Comparison with PrPSc-Infected Cell Screening. [PDF]

PLoS ONE, 2017
Prion propagation is mediated by the structural alteration of normal prion protein (PrPC) to generate pathogenic prion protein (PrPSc). To date, compounds for the inhibition of prion propagation have mainly been screened using PrPSc-infected cells.
Jae Wook Hyeon, Su Yeon Kim, Sol Moe Lee, Jeongmin Lee, Seong Soo A An, Myung Koo Lee, Yeong Seon Lee   +6 more
doaj   +1 more source

Evaluation of Real-Time Quaking-Induced Conversion, ELISA, and Immunohistochemistry for Chronic Wasting Disease Diagnosis

Frontiers in Veterinary Science, 2022
Chronic wasting disease (CWD) is a transmissible prion disorder, primarily affecting free-ranging and captive cervids in North America (United States and Canada), South Korea, and Europe (Finland, Norway, and Sweden).
Carine L. Holz, Joseph R. Darish, Kelly Straka, Nicole Grosjean, Steven Bolin, Matti Kiupel, Srinand Sreevatsan   +6 more
doaj   +1 more source

Early detection of abnormal prion protein in genetic human prion diseases now possible using real-time QUIC assay. [PDF]

PLoS ONE, 2013
INTRODUCTION: The definitive diagnosis of genetic prion diseases (gPrD) requires pathological confirmation. To date, diagnosis has relied upon the finding of the biomarkers 14-3-3 protein and total tau (t-tau) protein in the cerebrospinal fluid (CSF ...
Kazunori Sano, Katsuya Satoh, Ryuichiro Atarashi, Hiroshi Takashima, Yasushi Iwasaki, Mari Yoshida, Nobuo Sanjo, Hiroyuki Murai, Hidehiro Mizusawa, Matthias Schmitz, Inga Zerr, Yong-Sun Kim, Noriyuki Nishida   +12 more
doaj   +1 more source

Real-Time Quaking-Induced Conversion Detection of Bovine Spongiform Encephalopathy Prions in a Subclinical Steer

Frontiers in Veterinary Science, 2018
Bovine spongiform encephalopathy (BSE) belongs to a group of fatal prion diseases that result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that accumulates in the brain.
Soyoun Hwang, M. Heather West Greenlee, Anne Balkema-Buschmann, Martin H. Groschup, Eric M. Nicholson, Justin J. Greenlee   +5 more
doaj   +1 more source

Exploring physical and chemical factors influencing the properties of recombinant prion protein and the real-time quaking-induced conversion (RT-QuIC) assay. [PDF]

PLoS ONE, 2014
Real-time quaking-induced conversion (RT-QuIC), a highly specific and sensitive assay able to detect low levels of the disease-inducing isoform of the prion protein (PrP(d)) in brain tissue biopsies and cerebral spinal fluid, has great potential to ...
Keding Cheng, Angela Sloan, Kristen M Avery, Michael Coulthart, Michael Carpenter, J David Knox   +5 more
doaj   +1 more source