Diagnosis of prion diseases by RT-QuIC results in improved surveillance [PDF]
Neurology, 2020 To present the National Prion Disease Pathology Surveillance Center's (NPDPSC's) experience using CSF real-time quaking-induced conversion (RT-QuIC) as a diagnostic test, to examine factors associated with false-negative RT-QuIC results, and to investigate the impact of RT-QuICs on prion disease surveillance.Between May 2015 and April 2018, the NPDPSC ...
Daniel D. Rhoads +11 more
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THE DIAGNOSTIC UTILITY OF RT-QUIC IN SPORADIC CJD [PDF]
Journal of Neurology, Neurosurgery & Psychiatry, 2014 Sporadic CJD (sCJD) remains the commonest type of CJD. Currently, there is not a disease specific antemortem test available for the diagnosis of sCJD with postmortem remaining the definitive method of diagnosis. Current diagnostic criteria rely on clinical presentation in association with the MRI, EEG and CSF 14–3–3 protein.
Louise Davidson +4 more
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Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear
Scientific Reports, 2021 Efforts to contain the spread of chronic wasting disease (CWD), a fatal, contagious prion disease of cervids, would be aided by the availability of additional diagnostic tools.
Natalia C. Ferreira +13 more
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Anti-Prion Screening for Acridine, Dextran, and Tannic Acid using Real Time-Quaking Induced Conversion: A Comparison with PrPSc-Infected Cell Screening. [PDF]
PLoS ONE, 2017 Prion propagation is mediated by the structural alteration of normal prion protein (PrPC) to generate pathogenic prion protein (PrPSc). To date, compounds for the inhibition of prion propagation have mainly been screened using PrPSc-infected cells.
Jae Wook Hyeon +6 more
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Frontiers in Veterinary Science, 2022 Chronic wasting disease (CWD) is a transmissible prion disorder, primarily affecting free-ranging and captive cervids in North America (United States and Canada), South Korea, and Europe (Finland, Norway, and Sweden).
Carine L. Holz +6 more
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Exploring physical and chemical factors influencing the properties of recombinant prion protein and the real-time quaking-induced conversion (RT-QuIC) assay. [PDF]
PLoS ONE, 2014 Real-time quaking-induced conversion (RT-QuIC), a highly specific and sensitive assay able to detect low levels of the disease-inducing isoform of the prion protein (PrP(d)) in brain tissue biopsies and cerebral spinal fluid, has great potential to ...
Keding Cheng +5 more
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Early detection of abnormal prion protein in genetic human prion diseases now possible using real-time QUIC assay. [PDF]
PLoS ONE, 2013 INTRODUCTION: The definitive diagnosis of genetic prion diseases (gPrD) requires pathological confirmation. To date, diagnosis has relied upon the finding of the biomarkers 14-3-3 protein and total tau (t-tau) protein in the cerebrospinal fluid (CSF ...
Kazunori Sano +12 more
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Frontiers in Veterinary Science, 2018 Bovine spongiform encephalopathy (BSE) belongs to a group of fatal prion diseases that result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that accumulates in the brain.
Soyoun Hwang +5 more
doaj +1 more source
RT-QuIC Assays in Humans … and Animals.
Food Safety, 2016 Prion diseases are neurodegenerative diseases affecting both humans and animal species. The phenotypic spectrum is broad and includes Creutzfeldt-Jakob disease (CJD) and its variant zoonotic form (vCJD) in humans, while in animals, scrapie of sheep and goats, bovine spongiform encephalopathy and chronic wasting disease of deer, elk and moose are ...
Collins, Steven, Sarros, Shannon
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Use of bovine recombinant prion protein and real-time quaking-induced conversion to detect cattle transmissible mink encephalopathy prions and discriminate classical and atypical L- and H-Type bovine spongiform encephalopathy. [PDF]
PLoS ONE, 2017 Prions are amyloid-forming proteins that cause transmissible spongiform encephalopathies through a process involving conversion from the normal cellular prion protein to the pathogenic misfolded conformation (PrPSc).
Soyoun Hwang +2 more
doaj +1 more source