Misfolded alpha-synuclein detection by RT-QuIC in dementia with lewy bodies: a systematic review and meta-analysis [PDF]
Frontiers in Molecular Biosciences, 2023 Introduction: Dementia with Lewy Bodies (DLB) is the second most common cause of neurodegenerative dementia after Alzheimer’s disease (AD), but the field is still lacking a specific biomarker for its core pathology: alpha synuclein (α-syn).
Carmen Peña-Bautista +8 more
doaj +2 more sources
Development of a sensitive real-time quaking-induced conversion (RT-QuIC) assay for application in prion-infected blood. [PDF]
PLoS ONE, 2023 Efforts to prevent human-to-human transmission of variant Creutzfeldt-Jakob disease (vCJD) by contaminated blood would be aided by the development of a sensitive diagnostic test that could be routinely used to screen blood donations.
Charlotte M Thomas +7 more
doaj +2 more sources
RT-QuIC detection of tauopathies using full-length tau substrates [PDF]
Prion, 2020 Early detection and diagnosis of neurodegenerative diseases has been hampered by the lack of sensitive testing. Real-time quaking induced conversion (RT-QuIC) has been used for the early and sensitive detection of prion-induced neurologic disease, and has more recently been adapted to detect misfolded alpha-synuclein and tau as biomarkers for ...
Sarah Cooper +2 more
exaly +5 more sources
Detection of skin α-synuclein using RT-QuIC as a diagnostic biomarker for Parkinson’s disease in the Chinese population [PDF]
European Journal of Medical ResearchBackground Several studies have indicated that skin holds promise as a potential sample for detecting pathological α-Syn and serving as a diagnostic biomarker for α-synucleinopathies. Despite reports in Chinese PD patients, comprehensive research on skin
Jiaqi Li +10 more
doaj +2 more sources
Detection of CWD in cervids by RT-QuIC assay of third eyelids.
PLoS ONE, 2019 The diagnosis of chronic wasting disease (CWD) relies on demonstration of the disease-associated misfolded CWD prion protein (PrPCWD) in brain or retropharyngeal lymph node tissue by immunodetection methods, e.g. ELISA and immunohistochemistry (IHC). The
Sarah K Cooper +5 more
doaj +3 more sources
Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid [PDF]
Acta Neuropathologica CommunicationsThe real-time quaking-induced conversion (RT-QuIC) assay has revolutionized prion disease diagnosis by detecting amyloidogenic PrP conformers in different tissue types and body fluids.
Susana Da Silva Correia +12 more
doaj +2 more sources
α‐synuclein RT‐QuIC in cerebrospinal fluid of LRRK2‐linked Parkinson's disease [PDF]
Annals of Clinical and Translational Neurology, 2019 Background Leucine‐rich kinase 2 (LRRK2)‐linked Parkinson's disease (PD) is clinically indistinguishable from idiopathic PD (IPD). A pleiotropic neuropathology has been recognized but the majority of studies in LRRK2 p.G2019S patients reveal Lewy‐type ...
Alicia Garrido +5 more
doaj +5 more sources
Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC [PDF]
BMC Research Notes, 2018 Objective Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases, often referred as prion diseases. TSEs result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that ...
Soyoun Hwang +3 more
doaj +3 more sources
α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies [PDF]
Annals of Clinical and Translational Neurology, 2019 We applied RT‐QuIC assay to detect α‐synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt–Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases
Matilde Bongianni +18 more
doaj +6 more sources
Frontiers in Aging Neuroscience, 2022 The real-time quaking-induced conversion (RT-QuIC) assay has been developed and used as an in vitro diagnostic tool for Parkinson’s disease (PD). In this study, we established α-Syn RT-QuIC using recombinant human α-Syn as the substrate.
Dong-Dong Chen +12 more
doaj +1 more source