Ring trial of 2nd generation RT‐QuIC diagnostic tests for sporadic CJD [PDF]
Annals of Clinical and Translational Neurology, 2020 Objective Real‐time quaking‐induced conversion (RT‐QuIC) assays detect prion‐seeding activity in a variety of human biospecimens, including cerebrospinal fluid and olfactory mucosa swabs.
Christina D. Orrú +21 more
doaj +7 more sources
Development of a sensitive real-time quaking-induced conversion (RT-QuIC) assay for application in prion-infected blood. [PDF]
PLoS ONE, 2023 Efforts to prevent human-to-human transmission of variant Creutzfeldt-Jakob disease (vCJD) by contaminated blood would be aided by the development of a sensitive diagnostic test that could be routinely used to screen blood donations.
Charlotte M Thomas +7 more
doaj +2 more sources
Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson’s disease and dementia with Lewy bodies [PDF]
Acta Neuropathologica Communications, 2021 Definitive diagnosis of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) relies on postmortem finding of disease-associated alpha-synuclein (αSynD) as misfolded protein aggregates in the central nervous system (CNS). The recent development of
Connor Bargar +14 more
doaj +2 more sources
Detection of skin α-synuclein using RT-QuIC as a diagnostic biomarker for Parkinson’s disease in the Chinese population [PDF]
European Journal of Medical ResearchBackground Several studies have indicated that skin holds promise as a potential sample for detecting pathological α-Syn and serving as a diagnostic biomarker for α-synucleinopathies. Despite reports in Chinese PD patients, comprehensive research on skin
Jiaqi Li +10 more
doaj +2 more sources
Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid [PDF]
Acta Neuropathologica CommunicationsThe real-time quaking-induced conversion (RT-QuIC) assay has revolutionized prion disease diagnosis by detecting amyloidogenic PrP conformers in different tissue types and body fluids.
Susana Da Silva Correia +12 more
doaj +2 more sources
Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC [PDF]
BMC Research Notes, 2018 Objective Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases, often referred as prion diseases. TSEs result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that ...
Soyoun Hwang +3 more
doaj +3 more sources
α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies [PDF]
Annals of Clinical and Translational Neurology, 2019 We applied RT‐QuIC assay to detect α‐synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt–Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases
Matilde Bongianni +18 more
doaj +6 more sources
Frontiers in Aging Neuroscience, 2022 The real-time quaking-induced conversion (RT-QuIC) assay has been developed and used as an in vitro diagnostic tool for Parkinson’s disease (PD). In this study, we established α-Syn RT-QuIC using recombinant human α-Syn as the substrate.
Dong-Dong Chen +12 more
doaj +1 more source
Prion Seeding Activity in Plant Tissues Detected by RT-QuIC. [PDF]
PathogensPrion diseases such as scrapie, bovine spongiform encephalopathy (BSE), and chronic wasting disease (CWD) affect domesticated and wild herbivorous mammals. Animals afflicted with CWD, the transmissible spongiform encephalopathy of cervids (deer, elk, and moose), shed prions into the environment, where they may persist and remain infectious for years ...
Burgener K +5 more
europepmc +4 more sources
RT-QuIC: a highly promising diagnostic method for neurodegenerative diseases-advantages and limitations. [PDF]
Front NeurolAlthough it has been more than 200 years since Parkinson’s disease was described, we have not established biomarkers for its definitive diagnosis yet. Moreover, there is a similar case for the entire group of α-synucleinopathies, which are all characterized by the pathological accumulation of aggregated α-synuclein (α-Syn) in the brain and other ...
Koníčková D +7 more
europepmc +6 more sources