Advancing prion diagnostics: full-length human E200K RT-QuIC substrate facilitates prion detection in tear fluid and improves sensitivity in cerebrospinal fluid [PDF]
Acta Neuropathologica CommunicationsThe real-time quaking-induced conversion (RT-QuIC) assay has revolutionized prion disease diagnosis by detecting amyloidogenic PrP conformers in different tissue types and body fluids.
Susana Da Silva Correia +12 more
doaj +3 more sources
RT-QuIC Reactivity in the Brain and Other Organs and Tissues in Early, Middle-early, Middle-late and Terminal Stages of Scrapie Agent 263K Intracerebral Infection in Hamsters [PDF]
ZoonosesObjective: This study was aimed at analyzing the distribution and progression of prion seeds across the central nervous system (CNS) and peripheral tissues, by evaluating ...
Donglin Liang +8 more
doaj +4 more sources
Lancet Neurology, The, 2021 BACKGROUND Isolated rapid-eye-movement (REM) sleep behaviour disorder (IRBD) can be part of the prodromal stage of the α-synucleinopathies Parkinson's disease and dementia with Lewy bodies.
Álex Iranzo +2 more
exaly +2 more sources
RT-QuIC detection of tauopathies using full-length tau substrates [PDF]
Prion, 2020 Early detection and diagnosis of neurodegenerative diseases has been hampered by the lack of sensitive testing. Real-time quaking induced conversion (RT-QuIC) has been used for the early and sensitive detection of prion-induced neurologic disease, and has more recently been adapted to detect misfolded alpha-synuclein and tau as biomarkers for ...
Davin M Henderson, Edward A Hoover
exaly +5 more sources
Quantification of Lewy body pathology by cerebrospinal fluid endpoint dilution RT-QuIC in a neuropathological autopsy cohort of clinically heterogeneous participants. [PDF]
Acta NeuropatholThe identification of biomarkers predicting the burden of brain alpha-synuclein (α-syn) pathology in vivo represents a research priority in Lewy body disease (LBD).
Mastrangelo A +16 more
europepmc +2 more sources
Prion Positivity Detected by Real-Time Quaking-Induced Conversion (RT-QuIC) in the Cadaver of an Elderly Woman Subjected to Forensic Autopsy. [PDF]
CureusPrion diseases are fatal neurodegenerative disorders. Previous studies have indicated the presence of "prion carriers" who remain asymptomatic, but scrapie prion protein (PrPSc) has begun to accumulate in the brain.
Nakagaki T +8 more
europepmc +2 more sources
Human PrP E219K: a new and promising substrate for robust RT-QuIC amplification of human prions with potential for strain discrimination. [PDF]
Microbiol SpectrMammalian prion diseases are fatal neurodegenerative disorders caused by the conformational conversion of the host-encoded prion protein (PrP) into a pathogenic, misfolded isoform, known as PrPSc.
Marín-Moreno A +8 more
europepmc +2 more sources
RT-QuIC detection of chronic wasting disease prions in third eyelids from white-tailed deer. [PDF]
Sci RepChronic wasting disease (CWD) is a fatal neurodegenerative disease in cervids which is caused by prions, and new cases continue to appear in populations in North America and globally.
Høy-Petersen J +7 more
europepmc +2 more sources
Optimization of the Real-Time Quaking-Induced Conversion Assay for Prion Disease Diagnosis [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 The real-time quaking-induced conversion (RT-QuIC) assay is a highly reproducible and robust methodology exhibiting an excellent pre-mortem diagnostic accuracy for prion diseases.
Inga Zerr +11 more
doaj +3 more sources
RT-QuIC:a new test for sporadic CJD [PDF]
Practical Neurology, 2018 The diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) can be difficult, but the real-time quaking-induced conversion (RT-QuIC) assays have made a considerable impact on its clinical diagnosis.
Green, Alison J E
core +6 more sources