Results 41 to 50 of about 424,860 (186)
Optimization of the Real-Time Quaking-Induced Conversion Assay for Prion Disease Diagnosis
Frontiers in Bioengineering and Biotechnology, 2020 The real-time quaking-induced conversion (RT-QuIC) assay is a highly reproducible and robust methodology exhibiting an excellent pre-mortem diagnostic accuracy for prion diseases.
Inga Zerr +11 more
doaj +1 more source
Pathogens, 2022 Chronic wasting disease (CWD) is a transmissible prion disease of the cervidae family. ELISA and IHC tests performed postmortem on the medial retropharyngeal lymph nodes (RPLN) or obex are considered diagnostic gold standards for prion detection. However,
Catalina Picasso-Risso +6 more
doaj +1 more source
In Vivo Diagnosis of Synucleinopathies: A Comparative Study of Skin Biopsy and RT-QuIC. [PDF]
Neurology, 2021 To determine whether (1) immunofluorescence is a reproducible technique in detecting misfolded α-synuclein in skin nerves and subsequently whether (2) immunofluorescence and real-time quaking-induced conversion (RT-QuIC) (both in skin and CSF) show a comparable in vivo diagnostic accuracy in distinguishing synucleinopathies from non-synucleinopathies ...
Donadio V +12 more
europepmc +4 more sources
RT-QuIC seeding activity precedes IHC detection of PrPCWD in tonsil and RAMALT biopsies.
, 2020 Six, nine, twelve and fifteen-month biopsy samples of tonsil (black) and RAMALT (blue) were probed with anti-PrP antibody BAR224 for PrPCWD detection by IHC.
Lauren A. Bracchi (8452077) +7 more
core +1 more source
, 2022 This protocol is for the detection of prionoid alpha-synuclein forms in human cerebrospinal fluid using the Real Time Quacking-Induced Conversion method (RT-QuIC). The protocol is adapted from Marco J. Russo, Christina D. Orru, Luis Concha‑Marambio, Simone Giaisi et al., 2021 (doi:10.1186/s40478-021-01282-8) and Concha-Marambio et al., 2019 (doi:10 ...
openaire +1 more source
Progression of chronic wasting disease in white-tailed deer analyzed by serial biopsy RT-QuIC and immunohistochemistry. [PDF]
PLoS ONE, 2020 Chronic wasting disease (CWD) continues to spread or be recognized in the United States, Canada, and Europe. CWD is diagnosed by demonstration of the causative misfolded prion protein (PrPCWD) in either brain or lymphoid tissue using immunodetection ...
Davin M Henderson +7 more
doaj +1 more source
Translational Neurodegeneration, 2022 Background In patients with Parkinson’s disease (PD), real-time quaking-induced conversion (RT-QuIC) detection of pathological α-synuclein (α-syn) in olfactory mucosa (OM) is not as accurate as in other α-synucleinopathies.
Matilde Bongianni +25 more
doaj +1 more source
PAD-Beads enrichment enhances detection of PrPSc using real-time quaking-induced conversion
BMC Research Notes, 2019 Objective Scrapie is a transmissible spongiform encephalopathy (TSE) that naturally occurs in sheep and goats. This fatal neurodegenerative disease results from misfolding of the normal cellular prion protein (PrPC) to a pathogenic prion protein form ...
Soyoun Hwang +2 more
doaj +1 more source
RT-QuIC shows widespread detection of misfolded prion seeds beyond levels detected using IHC.
, 2016 (a) ThT fluorescence readout over time during the RT-QuIC assay. Each solid line represents a GSS/101LL brain region, whereas each dotted line represents a NBH/101LL brain region. These data are comprised of the averages from triplicate RT-QuIC reactions
Jean C. Manson (194761) +7 more
core +1 more source
Transmission of CJD from nasal brushings but not spinal fluid or RT‐QuIC product [PDF]
Annals of Clinical and Translational Neurology, 2020 AbstractObjectiveThe detection of prion seeding activity in CSF and olfactory mucosal brushings using real‐time quaking‐induced conversion assays allows highly accurate clinical diagnosis of sporadic Creutzfeldt–Jakob disease. To gauge transmission risks associated with these biospecimens and their testing, we have bioassayed prion infectivity levels ...
Raymond, Gregory J. +13 more
openaire +3 more sources