Results 61 to 70 of about 2,266 (154)
PLoS ONESeeding amplification assays, specifically the Real-Time Quaking-Induced Conversion method (RT-QuIC), have shown great diagnostic potential for α-synucleinopathies.
Remarh Bsoul +10 more
doaj +1 more source
PLoS ONE, 2019 Sporadic Creutzfeldt-Jakob disease (sCJD) is a transmissible, rapidly progressive and fatal neurodegenerative disease. The transmissible agent linked to sCJD is composed of the misfolded form of the host-encoded prion protein.
Gabriele Piconi +3 more
doaj +1 more source
A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility
Annals of Clinical and Translational Neurology, Volume 13, Issue 6, Page 1088-1105, June 2026.ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento +3 more
wiley +1 more source
Rapid and Sensitive RT-QuIC Detection of Human Creutzfeldt-Jakob Disease Using Cerebrospinal Fluid
mBio, 2015 Fast, definitive diagnosis of Creutzfeldt-Jakob disease (CJD) is important in assessing patient care options and transmission risks. Real-time quaking-induced conversion (RT-QuIC) assays of cerebrospinal fluid (CSF) and nasal-brushing specimens are ...
Christina D. Orrú +5 more
doaj +1 more source
CNS Neuroscience &Therapeutics, Volume 32, Issue 6, June 2026.This review systematically explores exercise as a multi‐target intervention for RBD, highlighting its mechanisms of action and regulatory effects on key pathophysiological processes. ABSTRACT Backgrounds REM sleep behavior disorder (RBD) is a prodromal non‐motor symptom of Parkinson's disease (PD) and other α‐synucleinopathies, affecting approximately ...
Xinhui Qiu +4 more
wiley +1 more source
Preparation of mouse tissue homogenates for RT-QuIC assay v1
, 2023 This protocol is designed for a standardized and efficient procedure to homogenize mouse tissue, conducive for RT-QuIC analysis. The process involves treating samples with PBS mixed with Triton-X100, followed by homogenization using a prob-tip sonicator.
arpine.sokratian not provided +1 more
openaire +1 more source
Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. [PDF]
PLoS Pathogens, 2010 A major problem for the effective diagnosis and management of prion diseases is the lack of rapid high-throughput assays to measure low levels of prions. Such measurements have typically required prolonged bioassays in animals.
Jason M Wilham +9 more
doaj +1 more source
, 2020 Abstract BackgroundThe definite diagnosis of human sporadic Creutzfeldt-Jakob disease (sCJD) largely depends on postmortem neuropathology and PrPSc detection in the brain. The development of prion RT-QuIC of cerebrospinal fluid (CSF) samples makes it possible for premortem diagnosis for sCJD.
Kang Xiao +7 more
openaire +1 more source
Nanoplastics and Neurodegeneration: A Roadmap From Mechanism to Causation
Advanced Science, Volume 13, Issue 27, 13 May 2026.Nanoplastics are pervasive environmental contaminants with potentially profound implications for human health. Emerging evidence suggests a possible link between nanoplastic exposure and neurodegeneration, a key driver of ageing and dementia, yet causality remains unresolved.
Yuhuan Li +5 more
wiley +1 more source
RT-QuIC: a new test for sporadic CJD [PDF]
Practical Neurology, 2018 The diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) can be difficult, but the real-time quaking-induced conversion (RT-QuIC) assays have made a considerable impact on its clinical diagnosis. This technique exploits the ability of the misfolded pathological form of prion protein (PrPSc) found in cerebrospinal fluid (CSF) to induce conversion of ...
openaire +3 more sources