Results 71 to 80 of about 424,860 (186)
A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility
Annals of Clinical and Translational Neurology, Volume 13, Issue 6, Page 1088-1105, June 2026.ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento +3 more
wiley +1 more source
PLoS ONESeeding amplification assays, specifically the Real-Time Quaking-Induced Conversion method (RT-QuIC), have shown great diagnostic potential for α-synucleinopathies.
Remarh Bsoul +10 more
doaj +1 more source
Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. [PDF]
PLoS Pathogens, 2010 A major problem for the effective diagnosis and management of prion diseases is the lack of rapid high-throughput assays to measure low levels of prions. Such measurements have typically required prolonged bioassays in animals.
Jason M Wilham +9 more
doaj +1 more source
CNS Neuroscience &Therapeutics, Volume 32, Issue 6, June 2026.This review systematically explores exercise as a multi‐target intervention for RBD, highlighting its mechanisms of action and regulatory effects on key pathophysiological processes. ABSTRACT Backgrounds REM sleep behavior disorder (RBD) is a prodromal non‐motor symptom of Parkinson's disease (PD) and other α‐synucleinopathies, affecting approximately ...
Xinhui Qiu +4 more
wiley +1 more source
, 2019 Amyloid seeding activity was detected by RT-QuIC in the 10−3–10−7 dilutions of CWD+ cervid brain pool CBP6 (2 μg-200 pg seed material; blue circles and blue median lines). sPMCA/RT-QuIC assay (round 5 sPMCA product diluted 1:100) detected amyloid seeding
Edward A. Hoover (369030) +6 more
core +1 more source
RT-QuIC sodium chloride titration for TME-infected and uninfected cattle brain samples.
, 2017 RT-QuIC reactions were seeded with 10−4 (A, B) and 10−6 dilution (C, D) of TME-infected and uninfected cattle brain homogenates using a range of NaCl concentrations (100–500 mM) with the full-length bPrP wild type (aa 25–241) as substrate.
Justin J. Greenlee (159291) +2 more
core +1 more source
RT-QuIC sodium chloride titration for different types of BSE-infected cattle brain samples.
, 2017 RT-QuIC reactions were seeded with 10−4 and 10−6 dilution of C-, L, and H-BSE-infected cattle brain homogenates using a range of NaCl concentrations (100–500 mM) with the full-length bPrP wild type (aa 25–241) as substrate. Data are presented as mean ThT
Justin J. Greenlee (159291) +2 more
core +1 more source
RT-QuIC: a new test for sporadic CJD [PDF]
Practical Neurology, 2018 The diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) can be difficult, but the real-time quaking-induced conversion (RT-QuIC) assays have made a considerable impact on its clinical diagnosis. This technique exploits the ability of the misfolded pathological form of prion protein (PrPSc) found in cerebrospinal fluid (CSF) to induce conversion of ...
openaire +3 more sources
Preparation of mouse tissue homogenates for RT-QuIC assay v1
, 2023 This protocol is designed for a standardized and efficient procedure to homogenize mouse tissue, conducive for RT-QuIC analysis. The process involves treating samples with PBS mixed with Triton-X100, followed by homogenization using a prob-tip sonicator.
arpine.sokratian not provided +1 more
openaire +1 more source
PLoS Pathogens, 2015 Prions propagate as multiple strains in a wide variety of mammalian species. The detection of all such strains by a single ultrasensitive assay such as Real Time Quaking-induced Conversion (RT-QuIC) would facilitate prion disease diagnosis, surveillance ...
Christina D Orrú +8 more
doaj +1 more source