Results 91 to 100 of about 616,892 (193)

Defining the Protein Seeds of Neurodegeneration using Real-Time Quaking-Induced Conversion Assays

Biomolecules, 2020
Neurodegenerative diseases are characterized by the accumulation of disease-related misfolded proteins. It is now widely understood that the characteristic self-amplifying (i.e., seeding) capacity once only attributed to the prions of transmissible ...
Matteo Manca, Allison Kraus
doaj   +1 more source

Enhanced Sensitivity of a Modified Quaking‐Induced Conversion Diagnostic Test for the Broad Detection of Sporadic and Inherited Prion Diseases: A Retrospective Study

Annals of Neurology, Volume 99, Issue 5, Page 1303-1314, May 2026.
Objective Quaking‐induced conversion (QuIC) tests, which detect prion‐seeding activity in cerebrospinal fluid (CSF), have markedly advanced the antemortem diagnosis of prion diseases such as Creutzfeldt‐Jakob disease (CJD). These tests provide high diagnostic accuracy and enable timely differentiation from other rapidly progressive neurodegenerative ...
Jennifer Myskiw, Rebecca Fox, Dominic M.S. Kielich, Lise Lamoureux, Melanie Leonhardt, Olivia Nykvist, Jessy A. Slota, Kristen Avery, Clark Phillipson, Kathy Frost, Sharon Simon, Brian S. Appleby, Ben A. Bailey‐Elkin, Stephanie A. Booth   +13 more
wiley   +1 more source

Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods [PDF]

, 2018
The concept that abnormal protein aggregates show prion-like propagation between cells has been considered to explain the onset and progression of many neurodegenerative diseases.
Arai Tetsuaki, Hasegawa Masato, Hisanaga Shin-ichi, Murayama Shigeo, Tarutani Airi, 新井 哲明   +5 more
core   +2 more sources

Transmission of CJD from nasal brushings but not spinal fluid or RT‐QuIC product [PDF]

Annals of Clinical and Translational Neurology, 2020
AbstractObjectiveThe detection of prion seeding activity in CSF and olfactory mucosal brushings using real‐time quaking‐induced conversion assays allows highly accurate clinical diagnosis of sporadic Creutzfeldt–Jakob disease. To gauge transmission risks associated with these biospecimens and their testing, we have bioassayed prion infectivity levels ...
Raymond, Gregory J., Race, Brent, Orrú, Christina D., Raymond, Lynne D., Bongianni, Matilde, Fiorini, Michele, Groveman, Bradley R., Ferrari, Sergio, Sacchetto, Luca, Hughson, Andrew G., Monaco, Salvatore, Pocchiari, Maurizio, Zanusso, Gianluigi, Caughey, Byron   +13 more
openaire   +3 more sources

Evidence for different seeding activities of misfolded tau in classical and rapidly progressive Alzheimer's disease

Brain Pathology, Volume 36, Issue 3, May 2026.
The study analyzed the seeding activity of misfolded tau protein in Alzheimer's disease and rapidly progressive Alzheimer's disease patients and provides evidence for the existence of different tau assemblies supported by differences in cellular toxicity and morphology of thioflavin T‐positive real‐time quaking‐induced conversion products.
Matthias Schmitz, Katrin Thüne, Anna‐Lisa Fischer, Peter Hermann, Maria Cramm, Leonie Sheila Kadel, Wiebke Möbius, Jakob Matschke, Tayyaba Saleem, Neelam Younas, Saima Zafar, Hermann Altmeppen, Markus Glatzel, Inga Zerr   +13 more
wiley   +1 more source

Skin as a promising biomatrix for the early detection of misfolded proteins by RT-QuIC in neurodegenerative diseases [PDF]

, 2023
Real-Time Quaking-Induced Conversion (RT-QuIC) is an ultrasensitive assay capable of detecting pathological aggregates of misfolded proteins in biospecimens.
Mammana, Angela <1994>
core   +1 more source

Evaluation of the impact of CSF prion RT-QuIC and amended criteria on the clinical diagnosis of Creutzfeldt-Jakob disease: a 10-year study in Italy

Journal of Neurology Neurosurgery & Psychiatry, 2022
Background The introduction of the prion Real-Time Quaking-Induced Conversion assay (RT-QuIC) has led to a revision of the diagnostic criteria for sporadic Creutzfeldt-Jakob disease (sCJD).
Andrea Mastrangelo, A. Mammana, Simone Baiardi, D. Tiple, Elisa Colaizzo, Marcello Rossi, Luana Vaianella, Barbara Polischi, M. Equestre, A. Poleggi, S. Capellari, A. Ladogana, Piero Parchi   +12 more
semanticscholar   +1 more source

Seeding amplification assay with Universal Control Fluid: Standardized detection of α-synucleinopathies.

PLoS ONE
Seeding amplification assays, specifically the Real-Time Quaking-Induced Conversion method (RT-QuIC), have shown great diagnostic potential for α-synucleinopathies.
Remarh Bsoul, Anja H Simonsen, Kristian S Frederiksen, Kirsten Svenstrup, Sara Bech, Lisette Salvesen, Anne-Mette Hejl, Marcello Rossi, Piero Parchi, Eva L Lund, Aušrinė Areškevičiūtė   +10 more
doaj   +1 more source

Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. [PDF]

PLoS Pathogens, 2010
A major problem for the effective diagnosis and management of prion diseases is the lack of rapid high-throughput assays to measure low levels of prions. Such measurements have typically required prolonged bioassays in animals.
Jason M Wilham, Christina D Orrú, Richard A Bessen, Ryuichiro Atarashi, Kazunori Sano, Brent Race, Kimberly D Meade-White, Lara M Taubner, Andrew Timmes, Byron Caughey   +9 more
doaj   +1 more source

Variably Protease‐Sensitive Prionopathy: Two New Cases With Motor Neuron‐Dementia Syndrome

Annals of Clinical and Translational Neurology, Volume 13, Issue 4, Page 827-833, April 2026.
ABSTRACT We describe two patients with variably protease‐sensitive prionopathy (VPSPr) who developed progressive upper motor neuron symptoms, insomnia, behavioral and cognitive decline, compatible with primary lateral sclerosis associated with frontotemporal dementia (FTD).
María Elena Erro, María Victoria Zelaya, Hasier Eraña, Javier Sánchez Ruiz de Gordoa, Fermín García‐Amigot, Anika Simonovska‐Serra, María Cristina Caballero, Isidre Ferrer, Ellen Gelpi, Ivonne Jericó, Joaquín Castilla   +10 more
wiley   +1 more source