Results 41 to 50 of about 2,160 (134)

α-Synuclein Real-Time Quaking-Induced Conversion Assay as a Biomarker for Parkinson's Disease: Clinical Implications and Perspectives. [PDF]

J Clin Neurol
Parkinson's disease (PD) has traditionally been diagnosed through clinical motor symptoms and the postmortem identification of pathological α-synuclein aggregates in Lewy bodies. The focus of diagnostic paradigms has recently moved from clinical approaches toward biological measures.
Yu JK, Lee YK, Yea DA, Kim YE.
europepmc   +3 more sources

Potential sources of interference with the highly sensitive detection and quantification of alpha‐synuclein seeds by qRT‐QuIC

FEBS Open Bio, 2020
Parkinson’s disease (PD) is a progressive neurodegenerative disease which is histologically characterized by loss of dopaminergic neurons in the substantia nigra and deposition of aggregated alpha‐synuclein (aSyn) in the brain.
Viktoria C. Ruf, Song Shi, Felix Schmidt, Daniel Weckbecker, Georg S. Nübling, Uwe Ködel, Brit Mollenhauer, Armin Giese   +7 more
doaj   +1 more source

Evaluation of the effect of various main elements on the PrPSc detection by real-time quaking-induced conversion assay. [PDF]

Int J Mol Med, 2018
In order to definitively diagnosis sporadic Creutzfeldt‑Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different variables, including the amount of recombinant celluar prion protein (rPrPC), salt, cleaning surfactants and thioflavin T ...
Xiao K, Shi Q, Zhou W, Gao C, Chen C, Zhang BY, Wang J, Dong XP.   +7 more
europepmc   +4 more sources

Real-Time Quaking-Induced Conversion Analysis for the Diagnosis of Sporadic Creutzfeldt-Jakob Disease in Korea [PDF]

Journal of Clinical Neurology, 2016
The level of 14-3-3 protein in the cerebrospinal fluid (CSF) is increased in Creutzfeldt-Jakob disease (CJD) patients, which has led to it being used as a clinical biomarker for the ante-mortem diagnosis of human prion diseases. However, the specificity of the 14-3-3 protein is less reliable for CJD diagnosis. Newly developed assays including real-time
Park, Jeong-Ho, Choi, Yeong-Gon, Lee, Yun-Jung, Park, Seok-Joo, Choi, Hong-Seok, Choi, Kyung-Chan, Choi, Eun-Kyoung, Kim, Yong-Sun   +7 more
openaire   +2 more sources

Estimating chronic wasting disease susceptibility in cervids using real-time quaking-induced conversion. [PDF]

J Gen Virol, 2017
In mammals, susceptibility to prion infection is primarily modulated by the host's cellular prion protein (PrPC) sequence. In the sheep scrapie model, a graded scale of susceptibility has been established both in vivo and in vitro based on PrPC amino acids 136, 154 and 171, leading to global breeding programmes to reduce the prevalence of scrapie in ...
Haley NJ, Rielinger R, Davenport KA, O'Rourke K, Mitchell G, Richt JA.   +5 more
europepmc   +3 more sources

Altered rPrP substrate structures and their influence on real-time quaking induced conversion reactions

Protein Expression and Purification, 2018
Bacterially-produced recombinant prion protein (rPrP) has traditionally been used for in vitro fibrillation assays and reagent development for prion disease research. In recent years, it has also been used as a substrate for real-time quaking-induced conversion (RT-QuIC), a very sensitive method of detecting the presence of the misfolded, disease ...
Keding Cheng, Angela Sloan, Brooks Waitt, Robert Vendramelli, Debra Godal, Sharon L.R. Simon, Joe O'Neil, Michael Carpenter, Dave Jackson, Jane Eastlake, Gary Mallinson, J. David Knox   +11 more
openaire   +2 more sources

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion

Nature Medicine, 2011
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted ...
Ryuichiro, Atarashi, Katsuya, Satoh, Kazunori, Sano, Takayuki, Fuse, Naohiro, Yamaguchi, Daisuke, Ishibashi, Takehiro, Matsubara, Takehiro, Nakagaki, Hitoki, Yamanaka, Susumu, Shirabe, Masahito, Yamada, Hidehiro, Mizusawa, Tetsuyuki, Kitamoto, Genevieve, Klug, Amelia, McGlade, Steven J, Collins, Noriyuki, Nishida   +16 more
openaire   +2 more sources

Early preclinical detection of prions in the skin of prion-infected animals

Nature Communications, 2019
There are currently no validated methods for the diagnosis of prion disease at the preclinical stage. Here the authors show that serial protein misfolding cyclic amplification and real-time quaking-induced conversion can be used to detect prions in the ...
Zerui Wang, Matteo Manca, Aaron Foutz, Manuel V. Camacho, Gregory J. Raymond, Brent Race, Christina D. Orru, Jue Yuan, Pingping Shen, Baiya Li, Yue Lang, Johnny Dang, Alise Adornato, Katie Williams, Nicholas R. Maurer, Pierluigi Gambetti, Bin Xu, Witold Surewicz, Robert B. Petersen, Xiaoping Dong, Brian S. Appleby, Byron Caughey, Li Cui, Qingzhong Kong, Wen-Quan Zou   +24 more
doaj   +1 more source

Quantification and diagnostic relevance of blood and heme-mediated inhibition of prion detection by RT-QuIC

Journal of Clinical Microbiology
Prion diseases are characterized by misfolding of prion protein (PrP) from correctly folded PrPC to a disease-associated form, PrPD. Real-time quaking-induced conversion (RT-QuIC) detects prions by “seeding” reaction mixtures, which contain recombinant ...
Robert B. Piel, David A. Schneider
doaj   +1 more source

Second-Generation RT-QuIC Assay for the Diagnosis of Creutzfeldt-Jakob Disease Patients in Brazil

Frontiers in Bioengineering and Biotechnology, 2020
The recent development of IQ-CSF, the second generation of real-time quaking-induced conversion (RT-QuIC) using cerebrospinal fluid (CSF), for the diagnosis of Creutzfeldt-Jakob Disease (CJD) represents a major diagnostic advance in the field.
Breno José Alencar Pires Barbosa, Bruno Batitucci Castrillo, Ricardo Pires Alvim, Marcelo Houat de Brito, Helio R. Gomes, Sônia M. D. Brucki, Jerusa Smid, Ricardo Nitrini, Michele C. Landemberger, Vilma R. Martins, Jerson L. Silva, Tuane C. R. G. Vieira   +11 more
doaj   +1 more source