Results 41 to 50 of about 20,909,028 (154)

Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories

Molecular Neurodegeneration, 2021
Background Detection of the pathological and disease-associated alpha-synuclein (αSynD) in the brain is required to formulate the definitive diagnosis of multiple system atrophy (MSA) and Parkinson’s disease (PD).
Connor Bargar, Chiara Maria Giulia De Luca, Grazia Devigili, Antonio Emanuele Elia, Roberto Cilia, Sara Maria Portaleone, Wen Wang, Irene Tramacere, Edoardo Bistaffa, Federico Angelo Cazzaniga, Giovanni Felisati, Giuseppe Legname, Alessio Di Fonzo, Rong Xu, Steven Alexander Gunzler, Giorgio Giaccone, Roberto Eleopra, Shu Guang Chen, Fabio Moda   +18 more
doaj   +1 more source

Seeding activity of human superoxide dismutase 1 aggregates in familial and sporadic amyotrophic lateral sclerosis postmortem neural tissues by real-time quaking-induced conversion. [PDF]

Acta Neuropathol
Amyotrophic lateral sclerosis (ALS) is a rapidly progressive neurodegenerative disease with average lifespan of 2-5 years after diagnosis. The identification of novel prognostic and pharmacodynamic biomarkers are needed to facilitate therapeutic ...
Mielke JK, Klingeborn M, Schultz EP, Markham EL, Reese ED, Alam P, Mackenzie IR, Ly CV, Caughey B, Cashman NR, Leavens MJ.   +10 more
europepmc   +2 more sources

α-Synuclein Real-Time Quaking-Induced Conversion Assay as a Biomarker for Parkinson's Disease: Clinical Implications and Perspectives. [PDF]

J Clin Neurol
Parkinson's disease (PD) has traditionally been diagnosed through clinical motor symptoms and the postmortem identification of pathological α-synuclein aggregates in Lewy bodies. The focus of diagnostic paradigms has recently moved from clinical approaches toward biological measures.
Yu JK, Lee YK, Yea DA, Kim YE.
europepmc   +3 more sources

Real time-quaking induced conversion assay (RT-QUIC) v1

, 2022
Seeded-amplification assay (SAA) method for the detection of pathology-associated aggregation-templating competent forms of alpha-synuclein and other interacting factors.
andrew.west west, Arpine Sokratian
openaire   +1 more source

Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC

BMC Research Notes, 2018
Objective Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases, often referred as prion diseases. TSEs result from the misfolding of the cellular prion protein (PrPC) into a pathogenic form (PrPSc) that ...
Soyoun Hwang, Trudy Tatum, Semakaleng Lebepe-Mazur, Eric M. Nicholson   +3 more
doaj   +1 more source

mCWD seeding activity in the spinal cord of Ndst1^f/ftga20^+/+SynCre- and SynCre+ mice using real-time quaking-induced conversion.

, 2023
mCWD seeding activity in the spinal cord of Ndst1f/ftga20+/+SynCre- and SynCre+ mice using real-time quaking-induced conversion.
Jeffrey J. Iliff (11808058), Adela Malik (17041664), Christopher Barback (6989393), Daniel R. Sandoval (10037976), Christina J. Sigurdson (180096), Christina D. Orrù (5651755), K. Peter R. Nilsson (8037491), Olivia R. Thomas (17041667), David R. Vera (1273554), Allison Kraus (602160), Ryotaro Ogawa (15018621), Byron Caughey (236055), Patricia Aguilar-Calvo (532439), Katrin Soldau (174488), Mckenzie B. Riley (17041670), Chrissa A. Dwyer (9197724), Jaidev Bapat (8282310), Heidi G. Standke (12981384), Jeffrey D. Esko (216312), Donald P. Pizzo (15004748)   +19 more
core   +1 more source

Atypical Case of VV1 Creutzfeldt–Jakob Disease Subtype: Case Report

Frontiers in Neurology, 2022
Creutzfeldt–Jakob disease (CJD) is a rare form of rapidly progressive, neurodegenerative disease that results from the misfolding and accumulation of an aberrant, disease-associated prion protein (PrPD).
Adrianna E. Carrasco, Brian S. Appleby, Ignazio Cali, Hamid R. Okhravi   +3 more
doaj   +1 more source

Real-time Quaking-induced Conversion Assay for the Diagnosis of Sporadic Creutzfeldt-Jakob Disease in a Living Patient. [PDF]

Infect Dis (Auckl), 2019
Creutzfeldt-Jakob disease (CJD) is the most common prion disease in humans with an incidence of one case per million inhabitants worldwide. The sporadic form of CJD (sCJD) is spontaneous and accounts for 85% of cases. Its symptoms include rapidly progressive dementia, ataxic gait, personality changes, myoclonus, coma, and eventually death.
Reddy VD, Hamed A, Settipalle N, Jande S, Rahman S, Szabella ME, Boghossian J.   +6 more
europepmc   +4 more sources

Real-Time Quaking-Induced Conversion Analysis for the Diagnosis of Sporadic Creutzfeldt-Jakob Disease in Korea [PDF]

Journal of Clinical Neurology, 2016
The level of 14-3-3 protein in the cerebrospinal fluid (CSF) is increased in Creutzfeldt-Jakob disease (CJD) patients, which has led to it being used as a clinical biomarker for the ante-mortem diagnosis of human prion diseases. However, the specificity of the 14-3-3 protein is less reliable for CJD diagnosis. Newly developed assays including real-time
Park, Jeong-Ho, Choi, Yeong-Gon, Lee, Yun-Jung, Park, Seok-Joo, Choi, Hong-Seok, Choi, Kyung-Chan, Choi, Eun-Kyoung, Kim, Yong-Sun   +7 more
openaire   +2 more sources

Potential sources of interference with the highly sensitive detection and quantification of alpha‐synuclein seeds by qRT‐QuIC

FEBS Open Bio, 2020
Parkinson’s disease (PD) is a progressive neurodegenerative disease which is histologically characterized by loss of dopaminergic neurons in the substantia nigra and deposition of aggregated alpha‐synuclein (aSyn) in the brain.
Viktoria C. Ruf, Song Shi, Felix Schmidt, Daniel Weckbecker, Georg S. Nübling, Uwe Ködel, Brit Mollenhauer, Armin Giese   +7 more
doaj   +1 more source