Results 51 to 60 of about 10,380 (154)

α-Synuclein Real-Time Quaking-Induced Conversion Assay as a Biomarker for Parkinson's Disease: Clinical Implications and Perspectives. [PDF]

J Clin Neurol
Parkinson's disease (PD) has traditionally been diagnosed through clinical motor symptoms and the postmortem identification of pathological α-synuclein aggregates in Lewy bodies. The focus of diagnostic paradigms has recently moved from clinical approaches toward biological measures.
Yu JK, Lee YK, Yea DA, Kim YE.
europepmc   +3 more sources

Real time-quaking induced conversion assay (RT-QUIC) v1

, 2022
Seeded-amplification assay (SAA) method for the detection of pathology-associated aggregation-templating competent forms of alpha-synuclein and other interacting factors.
andrew.west west, Arpine Sokratian
openaire   +1 more source

Potential sources of interference with the highly sensitive detection and quantification of alpha‐synuclein seeds by qRT‐QuIC

FEBS Open Bio, 2020
Parkinson’s disease (PD) is a progressive neurodegenerative disease which is histologically characterized by loss of dopaminergic neurons in the substantia nigra and deposition of aggregated alpha‐synuclein (aSyn) in the brain.
Viktoria C. Ruf, Song Shi, Felix Schmidt, Daniel Weckbecker, Georg S. Nübling, Uwe Ködel, Brit Mollenhauer, Armin Giese   +7 more
doaj   +1 more source

Median time to threshold of 20 white-tailed deer ears and belly skin samples determined to be CWD-positive by the real-time quaking-induced conversion (RT-QuIC) assay.

, 2022
Time to threshold was determined by the time at which fluorescent signal reaches 10× the standard deviation of the threshold (defined by the average of fluorescent readings of cycles 3–13).
Stuart S. Lichtenberg (14129070), Daniel P. Walsh (13985259), Kate R. Burgener (14129067), Joel A. Pedersen (171748), Aaron Lomax (14129073), Daniel J. Storm (5017322)   +5 more
core   +1 more source

Evaluation of the effect of various main elements on the PrPSc detection by real-time quaking-induced conversion assay. [PDF]

Int J Mol Med, 2018
In order to definitively diagnosis sporadic Creutzfeldt‑Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different variables, including the amount of recombinant celluar prion protein (rPrPC), salt, cleaning surfactants and thioflavin T ...
Xiao K, Shi Q, Zhou W, Gao C, Chen C, Zhang BY, Wang J, Dong XP.   +7 more
europepmc   +4 more sources

Brain and Cerebrospinal Fluid α-Synuclein Real-Time Quaking-Induced Conversion Identifies Lewy Body Pathology in LRRK2-PD [PDF]

, 2022
BACKGROUND: The neuropathology of Parkinson's disease (PD) associated with leucine-rich repeat kinase 2 (LRRK2) mutations (LRRK2-PD) is heterogeneous and varies with the type of mutation. There are only a few studies evaluating seeding aggregation assays
Garrido, Alicia, Maria J. Marti, Domenech, Maria Jose Marti, Alicia Garrido, Marti, Maria J, Tolosa, Eduardo, Fairfoul, Graham; id_orcid, Garrido-Pla, Alicia, Ezquerra, Mario, Mario Ezquerra, Eduardo Tolosa, Green, Alison J E, Alison J E Green, Green, Alison, Graham Fairfoul   +14 more
core   +1 more source

Real-Time Quaking-Induced Conversion Analysis for the Diagnosis of Sporadic Creutzfeldt-Jakob Disease in Korea [PDF]

Journal of Clinical Neurology, 2016
The level of 14-3-3 protein in the cerebrospinal fluid (CSF) is increased in Creutzfeldt-Jakob disease (CJD) patients, which has led to it being used as a clinical biomarker for the ante-mortem diagnosis of human prion diseases. However, the specificity of the 14-3-3 protein is less reliable for CJD diagnosis. Newly developed assays including real-time
Park, Jeong-Ho, Choi, Yeong-Gon, Lee, Yun-Jung, Park, Seok-Joo, Choi, Hong-Seok, Choi, Kyung-Chan, Choi, Eun-Kyoung, Kim, Yong-Sun   +7 more
openaire   +2 more sources

Estimating chronic wasting disease susceptibility in cervids using real-time quaking-induced conversion. [PDF]

J Gen Virol, 2017
In mammals, susceptibility to prion infection is primarily modulated by the host's cellular prion protein (PrPC) sequence. In the sheep scrapie model, a graded scale of susceptibility has been established both in vivo and in vitro based on PrPC amino acids 136, 154 and 171, leading to global breeding programmes to reduce the prevalence of scrapie in ...
Haley NJ, Rielinger R, Davenport KA, O'Rourke K, Mitchell G, Richt JA.   +5 more
europepmc   +3 more sources

Altered rPrP substrate structures and their influence on real-time quaking induced conversion reactions

Protein Expression and Purification, 2018
Bacterially-produced recombinant prion protein (rPrP) has traditionally been used for in vitro fibrillation assays and reagent development for prion disease research. In recent years, it has also been used as a substrate for real-time quaking-induced conversion (RT-QuIC), a very sensitive method of detecting the presence of the misfolded, disease ...
Keding Cheng, Angela Sloan, Brooks Waitt, Robert Vendramelli, Debra Godal, Sharon L.R. Simon, Joe O'Neil, Michael Carpenter, Dave Jackson, Jane Eastlake, Gary Mallinson, J. David Knox   +11 more
openaire   +2 more sources

Early preclinical detection of prions in the skin of prion-infected animals

Nature Communications, 2019
There are currently no validated methods for the diagnosis of prion disease at the preclinical stage. Here the authors show that serial protein misfolding cyclic amplification and real-time quaking-induced conversion can be used to detect prions in the ...
Zerui Wang, Matteo Manca, Aaron Foutz, Manuel V. Camacho, Gregory J. Raymond, Brent Race, Christina D. Orru, Jue Yuan, Pingping Shen, Baiya Li, Yue Lang, Johnny Dang, Alise Adornato, Katie Williams, Nicholas R. Maurer, Pierluigi Gambetti, Bin Xu, Witold Surewicz, Robert B. Petersen, Xiaoping Dong, Brian S. Appleby, Byron Caughey, Li Cui, Qingzhong Kong, Wen-Quan Zou   +24 more
doaj   +1 more source