Results 51 to 60 of about 20,909,028 (154)

Evaluation of the effect of various main elements on the PrPSc detection by real-time quaking-induced conversion assay. [PDF]

Int J Mol Med, 2018
In order to definitively diagnosis sporadic Creutzfeldt‑Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different variables, including the amount of recombinant celluar prion protein (rPrPC), salt, cleaning surfactants and thioflavin T ...
Xiao K, Shi Q, Zhou W, Gao C, Chen C, Zhang BY, Wang J, Dong XP.   +7 more
europepmc   +4 more sources

Median time to threshold of 20 white-tailed deer ears and belly skin samples determined to be CWD-positive by the real-time quaking-induced conversion (RT-QuIC) assay.

, 2022
Time to threshold was determined by the time at which fluorescent signal reaches 10× the standard deviation of the threshold (defined by the average of fluorescent readings of cycles 3–13).
Stuart S. Lichtenberg (14129070), Daniel P. Walsh (13985259), Kate R. Burgener (14129067), Joel A. Pedersen (171748), Aaron Lomax (14129073), Daniel J. Storm (5017322)   +5 more
core   +1 more source

Estimating chronic wasting disease susceptibility in cervids using real-time quaking-induced conversion. [PDF]

J Gen Virol, 2017
In mammals, susceptibility to prion infection is primarily modulated by the host's cellular prion protein (PrPC) sequence. In the sheep scrapie model, a graded scale of susceptibility has been established both in vivo and in vitro based on PrPC amino acids 136, 154 and 171, leading to global breeding programmes to reduce the prevalence of scrapie in ...
Haley NJ, Rielinger R, Davenport KA, O'Rourke K, Mitchell G, Richt JA.   +5 more
europepmc   +3 more sources

Altered rPrP substrate structures and their influence on real-time quaking induced conversion reactions

Protein Expression and Purification, 2018
Bacterially-produced recombinant prion protein (rPrP) has traditionally been used for in vitro fibrillation assays and reagent development for prion disease research. In recent years, it has also been used as a substrate for real-time quaking-induced conversion (RT-QuIC), a very sensitive method of detecting the presence of the misfolded, disease ...
Keding Cheng, Angela Sloan, Brooks Waitt, Robert Vendramelli, Debra Godal, Sharon L.R. Simon, Joe O'Neil, Michael Carpenter, Dave Jackson, Jane Eastlake, Gary Mallinson, J. David Knox   +11 more
openaire   +2 more sources

Brain and Cerebrospinal Fluid α-Synuclein Real-Time Quaking-Induced Conversion Identifies Lewy Body Pathology in LRRK2-PD [PDF]

, 2022
BACKGROUND: The neuropathology of Parkinson's disease (PD) associated with leucine-rich repeat kinase 2 (LRRK2) mutations (LRRK2-PD) is heterogeneous and varies with the type of mutation. There are only a few studies evaluating seeding aggregation assays
Garrido, Alicia, Maria J. Marti, Domenech, Maria Jose Marti, Alicia Garrido, Marti, Maria J, Tolosa, Eduardo, Fairfoul, Graham; id_orcid, Garrido-Pla, Alicia, Ezquerra, Mario, Mario Ezquerra, Eduardo Tolosa, Green, Alison J E, Alison J E Green, Green, Alison, Graham Fairfoul   +14 more
core   +1 more source

Early preclinical detection of prions in the skin of prion-infected animals

Nature Communications, 2019
There are currently no validated methods for the diagnosis of prion disease at the preclinical stage. Here the authors show that serial protein misfolding cyclic amplification and real-time quaking-induced conversion can be used to detect prions in the ...
Zerui Wang, Matteo Manca, Aaron Foutz, Manuel V. Camacho, Gregory J. Raymond, Brent Race, Christina D. Orru, Jue Yuan, Pingping Shen, Baiya Li, Yue Lang, Johnny Dang, Alise Adornato, Katie Williams, Nicholas R. Maurer, Pierluigi Gambetti, Bin Xu, Witold Surewicz, Robert B. Petersen, Xiaoping Dong, Brian S. Appleby, Byron Caughey, Li Cui, Qingzhong Kong, Wen-Quan Zou   +24 more
doaj   +1 more source

Quantification and diagnostic relevance of blood and heme-mediated inhibition of prion detection by RT-QuIC

Journal of Clinical Microbiology
Prion diseases are characterized by misfolding of prion protein (PrP) from correctly folded PrPC to a disease-associated form, PrPD. Real-time quaking-induced conversion (RT-QuIC) detects prions by “seeding” reaction mixtures, which contain recombinant ...
Robert B. Piel, David A. Schneider
doaj   +1 more source

Second-Generation RT-QuIC Assay for the Diagnosis of Creutzfeldt-Jakob Disease Patients in Brazil

Frontiers in Bioengineering and Biotechnology, 2020
The recent development of IQ-CSF, the second generation of real-time quaking-induced conversion (RT-QuIC) using cerebrospinal fluid (CSF), for the diagnosis of Creutzfeldt-Jakob Disease (CJD) represents a major diagnostic advance in the field.
Breno José Alencar Pires Barbosa, Bruno Batitucci Castrillo, Ricardo Pires Alvim, Marcelo Houat de Brito, Helio R. Gomes, Sônia M. D. Brucki, Jerusa Smid, Ricardo Nitrini, Michele C. Landemberger, Vilma R. Martins, Jerson L. Silva, Tuane C. R. G. Vieira   +11 more
doaj   +1 more source

Detection and Quantification of CWD Prions in Fixed Paraffin Embedded Tissues by Real-Time Quaking-Induced Conversion [PDF]

Scientific Reports, 2016
AbstractTraditional diagnostic detection of chronic wasting disease (CWD) relies on immunodetection of misfolded CWD prion protein (PrPCWD) by western blotting, ELISA, or immunohistochemistry (IHC). These techniques require separate sample collections (frozen and fixed) which may result in discrepancies due to variation in prion tissue distribution and
Clare E. Hoover, Kristen A. Davenport, Davin M. Henderson, Laura A. Pulscher, Candace K. Mathiason, Mark D. Zabel, Edward A. Hoover   +6 more
openaire   +2 more sources

Standardization of Data Analysis for RT-QuIC-Based Detection of Chronic Wasting Disease

Pathogens, 2023
Chronic wasting disease (CWD) is a disease affecting cervids and is caused by prions accumulating as pathogenic fibrils in lymphoid tissue and the central nervous system.
Gage R. Rowden, Catalina Picasso-Risso, Manci Li, Marc D. Schwabenlander, Tiffany M. Wolf, Peter A. Larsen   +5 more
doaj   +1 more source