Results 61 to 70 of about 10,380 (154)

Quantification and diagnostic relevance of blood and heme-mediated inhibition of prion detection by RT-QuIC

Journal of Clinical Microbiology
Prion diseases are characterized by misfolding of prion protein (PrP) from correctly folded PrPC to a disease-associated form, PrPD. Real-time quaking-induced conversion (RT-QuIC) detects prions by “seeding” reaction mixtures, which contain recombinant ...
Robert B. Piel, David A. Schneider
doaj   +1 more source

Second-Generation RT-QuIC Assay for the Diagnosis of Creutzfeldt-Jakob Disease Patients in Brazil

Frontiers in Bioengineering and Biotechnology, 2020
The recent development of IQ-CSF, the second generation of real-time quaking-induced conversion (RT-QuIC) using cerebrospinal fluid (CSF), for the diagnosis of Creutzfeldt-Jakob Disease (CJD) represents a major diagnostic advance in the field.
Breno José Alencar Pires Barbosa, Bruno Batitucci Castrillo, Ricardo Pires Alvim, Marcelo Houat de Brito, Helio R. Gomes, Sônia M. D. Brucki, Jerusa Smid, Ricardo Nitrini, Michele C. Landemberger, Vilma R. Martins, Jerson L. Silva, Tuane C. R. G. Vieira   +11 more
doaj   +1 more source

Standardization of Data Analysis for RT-QuIC-Based Detection of Chronic Wasting Disease

Pathogens, 2023
Chronic wasting disease (CWD) is a disease affecting cervids and is caused by prions accumulating as pathogenic fibrils in lymphoid tissue and the central nervous system.
Gage R. Rowden, Catalina Picasso-Risso, Manci Li, Marc D. Schwabenlander, Tiffany M. Wolf, Peter A. Larsen   +5 more
doaj   +1 more source

Detection and Quantification of CWD Prions in Fixed Paraffin Embedded Tissues by Real-Time Quaking-Induced Conversion [PDF]

Scientific Reports, 2016
AbstractTraditional diagnostic detection of chronic wasting disease (CWD) relies on immunodetection of misfolded CWD prion protein (PrPCWD) by western blotting, ELISA, or immunohistochemistry (IHC). These techniques require separate sample collections (frozen and fixed) which may result in discrepancies due to variation in prion tissue distribution and
Clare E. Hoover, Kristen A. Davenport, Davin M. Henderson, Laura A. Pulscher, Candace K. Mathiason, Mark D. Zabel, Edward A. Hoover   +6 more
openaire   +2 more sources

Estimation of prion infectivity in tissues of cattle infected with atypical BSE by real time-quaking induced conversion assay. [PDF]

J Vet Med Sci, 2019
Atypical bovine spongiform encephalopathy (BSE), first identified in 2004, poses a threat due to the potential to spread the disease to cattle and other animals, including humans. Here, we estimated prion titers in various tissues of cattle infected with atypical BSE using a real-time quaking-induced conversion assay that detects amyloid seeding ...
Sawada K, Suzuki A, Yamasaki T, Iwamaru Y, Matsuura Y, Miyazawa K, Masujin K, Atarashi R, Horiuchi M.   +8 more
europepmc   +4 more sources

A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility

Annals of Clinical and Translational Neurology, Volume 13, Issue 6, Page 1088-1105, June 2026.
ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento, Daniela F. Santos, Tuane C. R. G. Vieira, Tiago F. Outeiro   +3 more
wiley   +1 more source

Quantitative assessment of prion infectivity in tissues and body fluids by real-time quaking-induced conversion

Journal of General Virology, 2015
Prions are amyloid-forming proteins that cause transmissible spongiform encephalopathies through a process involving the templated conversion of the normal cellular prion protein (PrP(C)) to a pathogenic misfolded conformation. Templated conversion has been modelled in several in vitro assays, including serial protein misfolding amplification, amyloid ...
Davin M, Henderson, Kristen A, Davenport, Nicholas J, Haley, Nathaniel D, Denkers, Candace K, Mathiason, Edward A, Hoover   +5 more
openaire   +2 more sources

Exercise Intervention for REM Sleep Behavior Disorder in Parkinson's Disease: Mechanisms and Implications for Neurorehabilitation

CNS Neuroscience &Therapeutics, Volume 32, Issue 6, June 2026.
This review systematically explores exercise as a multi‐target intervention for RBD, highlighting its mechanisms of action and regulatory effects on key pathophysiological processes. ABSTRACT Backgrounds REM sleep behavior disorder (RBD) is a prodromal non‐motor symptom of Parkinson's disease (PD) and other α‐synucleinopathies, affecting approximately ...
Xinhui Qiu, Zihui Wang, Ruixue Xu, Yiying Hu, Weidong Le   +4 more
wiley   +1 more source

A direct assessment of human prion adhered to steel wire using real-time quaking-induced conversion [PDF]

Scientific Reports, 2016
AbstractAccidental transmission of prions during neurosurgery has been reported as a consequence of re-using contaminated surgical instruments. Several decontamination methods have been studied using the 263K-hamster prion; however, no studies have directly evaluated human prions.
Tsuyoshi Mori, Ryuichiro Atarashi, Kana Furukawa, Hanae Takatsuki, Katsuya Satoh, Kazunori Sano, Takehiro Nakagaki, Daisuke Ishibashi, Kazuko Ichimiya, Masahisa Hamada, Takehisa Nakayama, Noriyuki Nishida   +11 more
openaire   +2 more sources

Olfactory swab sampling optimization for α-synuclein aggregate detection in patients with Parkinson’s disease

Translational Neurodegeneration, 2022
Background In patients with Parkinson’s disease (PD), real-time quaking-induced conversion (RT-QuIC) detection of pathological α-synuclein (α-syn) in olfactory mucosa (OM) is not as accurate as in other α-synucleinopathies.
Matilde Bongianni, Mauro Catalan, Daniela Perra, Elena Fontana, Francesco Janes, Claudio Bertolotti, Luca Sacchetto, Stefano Capaldi, Matteo Tagliapietra, Paola Polverino, Valentina Tommasini, Giulia Bellavita, Elham Ataie Kachoie, Roberto Baruca, Andrea Bernardini, Mariarosaria Valente, Michele Fiorini, Erika Bronzato, Stefano Tamburin, Laura Bertolasi, Lorenzo Brozzetti, Maria Paola Cecchini, Gianluigi Gigli, Salvatore Monaco, Paolo Manganotti, Gianluigi Zanusso   +25 more
doaj   +1 more source