Results 51 to 60 of about 878,005 (252)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Impact of inter- and intra-individual variation, sample storage and sampling fraction on human stool microbial community profiles [PDF]

PeerJ, 2019
Stools are commonly used as proxies for studying human gut microbial communities as sample collection is straightforward, cheap and non-invasive.
Yun Kit Yeoh, Zigui Chen, Mamie Hui, Martin C.S. Wong, Wendy C.S. Ho, Miu Ling Chin, Siew C. Ng, Francis K.L. Chan, Paul K.S. Chan   +8 more
doaj   +2 more sources

An Optimized Approach for Serial Crystallography Using Chips

Crystals, 2023
Serial crystallography is a rapidly developing method for the determination of the structure of biomolecules at room temperature at near-atomic resolution from an ensemble of small crystals. Numerous advances in detectors, data analysis pipelines, sample
Marina Galchenkova, Aida Rahmani Mashhour, Patrick Y. A. Reinke, Sebastian Günther, Jan Meyer, Henry N. Chapman, Oleksandr M. Yefanov   +6 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Small but mighty: the power of microcrystals in structural biology

IUCrJ
Advancements in macromolecular crystallography, driven by improved sources and cryocooling techniques, have enabled the use of increasingly smaller crystals for structure determination, with microfocus beamlines now widely accessible. Initially developed
Courtney J. Tremlett, Jack Stubbs, William S. Stuart, Patrick D. Shaw Stewart, Jonathan West, Allen M. Orville, Ivo Tews, Nicholas J. Harmer   +7 more
doaj   +1 more source

A model for rapid, active surveillance for medically-attended acute gastroenteritis within an integrated health care delivery system. [PDF]

PLoS ONE, 2018
BACKGROUND:This study presents a novel methodology for estimating all-age, population-based incidence rates of norovirus and other pathogens that contribute to acute gastroenteritis in the United States using an integrated healthcare delivery system as a
Mark A Schmidt, Holly C Groom, Allison L Naleway, Christianne Biggs, S Bianca Salas, Kayoko Shioda, Zachary Marsh, Judy L Donald, Aron J Hall   +8 more
doaj   +1 more source

A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples

Micromachines
Conventional laboratory methods for handling valuable biological samples typically use pipettes or needles, which are prone to issues such as dead volume and sample waste. Furthermore, the sampling and processing of pathogenic bacteria, such as Escherichia coli (E.
Ziyang Han, Pengfei Gong, Hengxiang Su, Zehang Gao, Shilun Feng, Jianlong Zhao   +5 more
openaire   +3 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source