Structure of the SAS-6 cartwheel hub from Leishmania major [PDF]
eLife, 2014 Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the ...
Mark van Breugel +4 more
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Pericentrin-mediated SAS-6 recruitment promotes centriole assembly [PDF]
eLife, 2019 The centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar material (PCM). Although centrioles are known to regulate PCM assembly, it is less known whether and how the PCM contributes to centriole assembly. Here we
Daisuke Ito +8 more
doaj +6 more sources
Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly [PDF]
Nature Communications, 2021 Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. Here, the authors develop monobodies against Chlamydomonas reinhardtii SAS-6 and
Georgios N. Hatzopoulos +9 more
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Poc1B and Sas-6 Function Together during the Atypical Centriole Formation in Drosophila melanogaster [PDF]
Cells, 2019 Insects and mammals have atypical centrioles in their sperm. However, it is unclear how these atypical centrioles form. Drosophila melanogaster sperm has one typical centriole called the giant centriole (GC) and one atypical centriole called the proximal
Kyoung H. Jo +5 more
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Dynamic SAS-6 phosphorylation aids centrosome duplication and elimination in C. elegans oogenesis [PDF]
EMBO ReportsIn most metazoans, centrosome elimination during oogenesis ensures accurate centriole inheritance in the zygote, yet the molecular mechanisms remain poorly understood.
Feifei Qi +15 more
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The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies [PDF]
eLife, 2015 Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved,
Matthew A Cottee +5 more
doaj +5 more sources
Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture [PDF]
Nature Communications, 2021 The centriole exhibits an evolutionarily conserved 9-fold radial symmetry that stems from a cartwheel containing vertically stacked ring polymers that harbor 9 homodimers of the protein SAS-6. Here the authors show how dual properties inherent to surface-
Niccolò Banterle +11 more
doaj +7 more sources
Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells [PDF]
eLife, 2022 SAS-6 (SASS6) is essential for centriole formation in human cells and other organisms but its functions in the mouse are unclear. Here, we report that Sass6-mutant mouse embryos lack centrioles, activate the mitotic surveillance cell death pathway, and ...
Marta Grzonka, Hisham Bazzi
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De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly [PDF]
eLife, 2015 Vertebrate centrioles normally propagate through duplication, but in the absence of preexisting centrioles, de novo synthesis can occur. Consistently, centriole formation is thought to strictly rely on self-assembly, involving self-oligomerization of the
Won-Jing Wang +5 more
doaj +2 more sources
The chromatin remodeling protein CHD-1 and the EFL-1/DPL-1 transcription factor cooperatively down regulate CDK-2 to control SAS-6 levels and centriole number. [PDF]
PLoS Genetics, 2022 Centrioles are submicron-scale, barrel-shaped organelles typically found in pairs, and play important roles in ciliogenesis and bipolar spindle assembly.
Jyoti Iyer +10 more
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