Results 11 to 20 of about 497,131 (99)

Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel. [PDF]

Structure, 2022
Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9-fold radial symmetry imparted by a likewise symmetrical central scaffold, the cartwheel.
Kantsadi AL, Hatzopoulos GN, Gönczy P, Vakonakis I.   +3 more
europepmc   +4 more sources

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly. [PDF]

Nat Commun, 2021
AbstractCentrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation.
Hatzopoulos GN, Kükenshöner T, Banterle N, Favez T, Flückiger I, Hamel V, Andany S, Fantner GE, Hantschel O, Gönczy P.   +9 more
europepmc   +7 more sources

Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. [PDF]

Elife, 2019
The centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar material (PCM). Although centrioles are known to regulate PCM assembly, it is less known whether and how the PCM contributes to centriole assembly. Here we investigate the interaction between centriole components and the PCM by taking advantage of fission
Ito D, Zitouni S, Jana SC, Duarte P, Surkont J, Carvalho-Santos Z, Pereira-Leal JB, Ferreira MG, Bettencourt-Dias M.   +8 more
europepmc   +6 more sources

Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells. [PDF]

Elife, 2022
Abstract Cell division fidelity is crucial for stem cell propagation and the maintenance of pluripotency. Centrosomes, which organize the mitotic spindle microtubules to ensure proper bipolar cell division, have a core of a pair of centrioles that duplicate once per cell cycle.
Grzonka M, Bazzi H.
europepmc   +4 more sources

Identification of compounds that bind the centriolar protein SAS-6 and inhibit its oligomerization. [PDF]

J Biol Chem, 2020
Centrioles are key eukaryotic organelles that are responsible for the formation of cilia and flagella, and for organizing the microtubule network and the mitotic spindle in animals. Centriole assembly requires oligomerization of the essential protein spindle assembly abnormal 6 (SAS-6), which forms a structural scaffold templating the organization of ...
Busch JMC, Matsoukas MT, Musgaard M, Spyroulias GA, Biggin PC, Vakonakis I.   +5 more
europepmc   +5 more sources

The PLK4-STIL-SAS-6 module at the core of centriole duplication. [PDF]

Biochem Soc Trans, 2016
Centrioles are microtubule-based core components of centrosomes and cilia. They are duplicated exactly once during S-phase progression. Central to formation of each new (daughter) centriole is the formation of a nine-fold symmetrical cartwheel structure onto which microtubule triplets are deposited.
Arquint C, Nigg EA.
europepmc   +9 more sources

Generation of sas-6::ha by CRISPR/Cas9 editing. [PDF]

MicroPubl Biol, 2019
Centrosomes are comprised of a pair of barrel-shaped centrioles that are oriented at right angles to each other, and embedded in electron dense pericentriolar material. The centrosomes mediate spindle assembly and function as basal bodies to promote cilia and flagella formation (reviewed in Pintard and Bowerman, 2019 and Fırat-Karalar and Stearns, 2014)
Bergwell M, Smith A, Lakin H, Slay R, Iyer J.   +4 more
europepmc   +3 more sources

Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly. [PDF]

Dev Cell, 2013
Assembly of SAS-6 dimers to form the centriolar cartwheel requires the ZYG-1/Plk4 kinase. Here, we show that ZYG-1 recruits SAS-6 to the mother centriole independently of its kinase activity; kinase activity is subsequently required for cartwheel assembly. We identify a direct interaction between ZYG-1 and the SAS-6 coiled coil that explains its kinase
Lettman MM, Wong YL, Viscardi V, Niessen S, Chen SH, Shiau AK, Zhou H, Desai A, Oegema K.   +8 more
europepmc   +4 more sources

Structure of the SAS-6 cartwheel hub from Leishmania major. [PDF]

Elife, 2014
Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole
van Breugel M, Wilcken R, McLaughlin SH, Rutherford TJ, Johnson CM.   +4 more
europepmc   +6 more sources

Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture. [PDF]

Nat Commun, 2021
Abstract Discovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates.
Banterle N, Nievergelt AP, de Buhr S, Hatzopoulos GN, Brillard C, Andany S, Hübscher T, Sorgenfrei FA, Schwarz US, Gräter F, Fantner GE, Gönczy P.   +11 more
europepmc   +7 more sources