SAS-6 oligomerization: the key to the centriole?
Nature Chemical Biology, 2011 Centrioles are among the most beautiful of biological structures. How their highly conserved nine-fold symmetry is generated is a question that has intrigued cell biologists for decades. Two recent structural studies provide the tantalizing suggestion that the self-organizing properties of the SAS-6 protein hold the answer.
Cottee, M, Raff, J, Lea, S, Roque, H
openaire +3 more sources
Identification and localization of SAS-6 in the microsporidium Nosema bombycis
Infection, Genetics and Evolution, 2019 The centriole in eukaryotes functions as the cell's microtubule-organizing center (MTOC) to nucleate spindle assembly. The evolutionarily conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis.
Weijiang, Dai +8 more
openaire +2 more sources
Phosphorylation of SAS-6 by ZYG-1 Is Critical for Centriole Formation in C. elegans Embryos [PDF]
Developmental Cell, 2009 Despite being essential for proper cell division, the mechanisms governing centrosome duplication are incompletely understood and represent an important open question in cell biology. Formation of a new centriole next to each existing one is critical for centrosome duplication. In Caenorhabditis elegans embryos, the proteins SPD-2, ZYG-1, SAS-6, SAS-5,
Kitagawa D +3 more
openaire +3 more sources
SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture [PDF]
Nature Cell Biology, 2016 Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS-6-based oligomers with symmetries ranging from five- to ten-fold.
Hilbert, Manuel +17 more
openaire +3 more sources
SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells [PDF]
Current Biology, 2020 Centrioles are essential components of centrosome, the main microtubule-organizing center of animal cells required for robust spindle bipolarity [1, 2]. They are duplicated once during the cell cycle [3], and the duplication involves assembly of a cartwheel on the pre-existing centriole followed by assembly of triplet microtubules around the cartwheel [
Hindol, Gupta +8 more
openaire +2 more sources
Coupling Form and Function: How the Oligomerisation Symmetry of the SAS-6 Protein Contributes to the Architecture of Centriole Organelles [PDF]
Symmetry, 2017 Centrioles make up the centrosome and basal bodies in animals and as such play important roles in cell division, signalling and motility. They possess characteristic 9-fold radial symmetry strongly influenced by the protein SAS-6. SAS-6 is essential for canonical centriole assembly as it forms the central core of the organelle, which is then surrounded
Jodie E. Ford +2 more
openaire +2 more sources
A primary microcephaly-associated sas-6 mutation perturbs centrosome duplication, dendrite morphogenesis, and ciliogenesis in Caenorhabditis elegans. [PDF]
Genetics, 2023 Bergwell M +10 more
europepmc +1 more source
The chromatin remodeling protein CHD-1 and the EFL-1/DPL-1 transcription factor cooperatively down regulate CDK-2 to control SAS-6 levels and centriole number. [PDF]
PLoS Genet, 2022 Iyer J +10 more
europepmc +1 more source
Re-engineering Caenorhabditis elegans SAS-6: from spirals to rings
, 2019 Centrioles have conserved 9-fold symmetry. This symmetry is templated by a scaffold of SAS-6. SAS-6 oligomerises to form a stack of rings, each with 9-fold radial symmetry, that forms the cartwheel, which imparts symmetry to the organelle. However, crystallographic and electron microscopy studies suggest that C.
openaire +1 more source
How to Break a Ring: Exploring the Mechanisms of SAS-6 Oligomerisation [PDF]
Biophysical Journal, 2017 Julia M.C. Busch +3 more
openaire +1 more source