Results 21 to 30 of about 497,131 (99)

Self-assembling SAS-6 multimer is a core centriole building block. [PDF]

J Biol Chem, 2010
Centrioles are conserved microtubule-based organelles with 9-fold symmetry that are essential for cilia and mitotic spindle formation. A conserved structure at the onset of centriole assembly is a "cartwheel" with 9-fold radial symmetry and a central tubule in its core. It remains unclear how the cartwheel is formed.
Gopalakrishnan J, Guichard P, Smith AH, Schwarz H, Agard DA, Marco S, Avidor-Reiss T.   +6 more
europepmc   +5 more sources

Poc1B and Sas-6 Function Together during the Atypical Centriole Formation in Drosophila melanogaster. [PDF]

Cells, 2019
Insects and mammals have atypical centrioles in their sperm. However, it is unclear how these atypical centrioles form. Drosophila melanogaster sperm has one typical centriole called the giant centriole (GC) and one atypical centriole called the proximal centriole-like structure (PCL).
Jo KH, Jaiswal A, Khanal S, Fishman EL, Curry AN, Avidor-Reiss T.   +5 more
europepmc   +5 more sources

Dynamic SAS-6 phosphorylation aids centrosome duplication and elimination in C. elegans oogenesis. [PDF]

EMBO Rep
Abstract In most metazoans, centrosome elimination during oogenesis ensures accurate centriole inheritance in the zygote, yet the molecular mechanisms remain poorly understood. Here, we reveal a critical role for controlled SAS-6 phosphorylation in centrosome dynamics during oogenesis.
Qi F, Yin S, Yang X, Ju N, Liu B, Zhang X, Zhu Z, Ji L, Zhang F, Zhao L, Wang R, Liu M, Zhang L, Zhao H, Zhou J, Gao J.   +15 more
europepmc   +3 more sources

The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. [PDF]

Elife, 2015
Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD).
Cottee MA, Muschalik N, Johnson S, Leveson J, Raff JW, Lea SM.   +5 more
europepmc   +5 more sources

SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication. [PDF]

Dev Cell, 2014
Centrioles are 9-fold symmetric structures duplicating once per cell cycle. Duplication involves self-oligomerization of the centriolar protein SAS-6, but how the 9-fold symmetry is invariantly established remains unclear. Here, we found that SAS-6 assembly can be shaped by preexisting (or mother) centrioles.
Fong CS, Kim M, Yang TT, Liao JC, Tsou MF.   +4 more
europepmc   +5 more sources

A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components. [PDF]

Eukaryot Cell, 2013
ABSTRACTSAS-6 is required for centriole biogenesis in diverse eukaryotes. Here, we describe a novel family of SAS-6-like (SAS6L) proteins that share an N-terminal domain with SAS-6 but lack coiled-coil tails. SAS6L proteins are found in a subset of eukaryotes that contain SAS-6, including diverse protozoa and green algae.
de Leon JC, Scheumann N, Beatty W, Beck JR, Tran JQ, Yau C, Bradley PJ, Gull K, Wickstead B, Morrissette NS.   +9 more
europepmc   +7 more sources

A dynamically interacting flexible loop assists oligomerisation of the Caenorhabditis elegans centriolar protein SAS-6. [PDF]

Sci Rep, 2019
AbstractCentrioles are conserved organelles fundamental for the organisation of microtubules in animal cells. Oligomerisation of the spindle assembly abnormal protein 6 (SAS-6) is an essential step in the centriole assembly process and may act as trigger for the formation of these organelles.
Busch JMC, Erat MC, Blank ID, Musgaard M, Biggin PC, Vakonakis I.   +5 more
europepmc   +5 more sources

Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry. [PDF]

Proc Natl Acad Sci U S A, 2013
Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown
Hilbert M, Erat MC, Hachet V, Guichard P, Blank ID, Flückiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gönczy P, Vakonakis I.   +11 more
europepmc   +6 more sources

SAS-6 is a Cartwheel Protein that Establishes the 9-Fold Symmetry of the Centriole [PDF]

Current Biology, 2007
Centrioles consist of nine-triplet microtubules arranged in rotational symmetry. This structure is highly conserved among various eukaryotic organisms and serves as the base for the ciliary axoneme. Recently, several proteins such as SAS-6 have been identified as essential to the early process of centriole assembly, but the mechanism that produces the ...
Nakazawa, Yuki, Hiraki, Madoka, Kamiya, Ritsu, Hirono, Masafumi   +3 more
openaire   +2 more sources

Structures of SAS-6 Suggest Its Organization in Centrioles

Science, 2011
Self-assembly of a centriolar protein may contribute to organizing the cartwheel-like hub and establishing centriole symmetry.
van Breugel, Mark, Hirono, Masafumi, Andreeva, Antonina, Yanagisawa, Haru-aki, Yamaguchi, Shoko, Nakazawa, Yuki, Morgner, Nina, Petrovich, Miriana, Ebong, Ima-Obong, Robinson, Carol V, Johnson, Christopher M, Veprintsev, Dmitry, Zuber, Benoît   +12 more
openaire   +4 more sources