Results 181 to 190 of about 35,719 (245)
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Protein and Peptide Letters, 2018
The most common recombinant antibody format is the single chain fragment variable (scFv) which it contains the complete antigen-binding domains of an intact antibody. ScFv fragments have found vast medical and non-medical applications. Several approaches have been employed to increase the affinity, avidity and structural stability related to these ...
Peyman Bemani, Mozafar Mohammadi
exaly +4 more sources
The most common recombinant antibody format is the single chain fragment variable (scFv) which it contains the complete antigen-binding domains of an intact antibody. ScFv fragments have found vast medical and non-medical applications. Several approaches have been employed to increase the affinity, avidity and structural stability related to these ...
Peyman Bemani, Mozafar Mohammadi
exaly +4 more sources
Deciphering Protein Corona by scFv-Based Affinity Chromatography.
Nano letters (Print), 2021It remains challenging to precisely decipher the structural and functional characteristics of protein coronas. To overcome the drawbacks frequently occurring in the traditional separation methods, an anti-PEG single-chain variable fragment (PEG-scFv ...
Yuxiu Chu +8 more
semanticscholar +1 more source
Biochemical and Biophysical Research Communications - BBRC, 2020
Gene-modifying T cells expressing chimeric antigen receptor (CAR) with an extracellular domain consisting of single chain variable fragment (scFv) and an intracellular domain with a T cell activation motif, are promising cancer immuno-medicines that can ...
K. Fujiwara +3 more
semanticscholar +1 more source
Gene-modifying T cells expressing chimeric antigen receptor (CAR) with an extracellular domain consisting of single chain variable fragment (scFv) and an intracellular domain with a T cell activation motif, are promising cancer immuno-medicines that can ...
K. Fujiwara +3 more
semanticscholar +1 more source
Anti-PEG scFv corona ameliorates accelerated blood clearance phenomenon of PEGylated nanomedicines.
Journal of Controlled Release, 2020Anti-PEG antibodies have been witnessed in patients and experimental animals, accelerating the blood clearance (termed ABC phenomenon) of PEGylated nanomedicines by activating complement after absorption on the nano-surface.
Zui Zhang +8 more
semanticscholar +1 more source
Cell Biochemistry and Biophysics, 2013
Osteoporosis is a systemic metabolic disease characterized by low bone mass with deterioration of the bony microstructure which leads to both bone brittleness and increased risk of fracture. Sclerostin is a protein encoded by the SOST gene which is specifically expressed in osteocyte.
Qi, Yao +5 more
openaire +2 more sources
Osteoporosis is a systemic metabolic disease characterized by low bone mass with deterioration of the bony microstructure which leads to both bone brittleness and increased risk of fracture. Sclerostin is a protein encoded by the SOST gene which is specifically expressed in osteocyte.
Qi, Yao +5 more
openaire +2 more sources
Reformatting of scFv Antibodies into the scFv-Fc Format and Their Downstream Purification
2014The scFv-Fc format allows for rapid characterization of candidate scFvs isolated from phage display libraries before conversion into a full-length IgG. This format offers several advantages over the phage display-derived scFv, including bivalent binding, longer half-life, and Fc-mediated effector functions.
Emil, Bujak +3 more
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Enzymatic Assembly for scFv Library Construction
2017Recombinant monoclonal antibodies can be established by displaying single-chain variable fragment (scFv) antibody libraries on phages and then biopanning against the target. For constructing superior scFv libraries, antibody light-chain variable region (VL) and heavy-chain variable region (VH) fragments must be assembled into scFvs without loss of ...
Mieko, Kato, Yoshiro, Hanyu
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Parallelized Microscale Expression of Soluble scFv
2019Antibody phage display is a key technology to generate recombinant, mainly human, antibodies for diagnostic and therapy, but also as tools for basic research. After antibody selection by "panning," a crucial step is the screening of monoclonal binders to isolate those which show antigen specificity.
Giulio, Russo +4 more
openaire +2 more sources