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Enzymatic Assembly for scFv Library Construction
2017Recombinant monoclonal antibodies can be established by displaying single-chain variable fragment (scFv) antibody libraries on phages and then biopanning against the target. For constructing superior scFv libraries, antibody light-chain variable region (VL) and heavy-chain variable region (VH) fragments must be assembled into scFvs without loss of ...
Mieko, Kato, Yoshiro, Hanyu
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Parallelized Microscale Expression of Soluble scFv
2019Antibody phage display is a key technology to generate recombinant, mainly human, antibodies for diagnostic and therapy, but also as tools for basic research. After antibody selection by "panning," a crucial step is the screening of monoclonal binders to isolate those which show antigen specificity.
Giulio, Russo +4 more
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ScFv-CH3 and scFv-Fc Fusion Proteins
2001Earlier chapters (Chapters 2–16) have presented methods for producing small engineered antibodies (notably, scFv) from hybridomas or through selection from combinatorial libraries. It is now possible to generate fragments with a vast spectrum of specificities, including against targets previously inaccessible through hybridoma technology.
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Journal of Immunological Methods, 2001
M13 scFv display libraries are frontline research tools due to the rapidity in which target binding scFv can be obtained. Simple modifications of the standard affinity selection and amplification protocol allow for multiple targets to be panned simultaneously.
J, Castillo, B, Goodson, J, Winter
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M13 scFv display libraries are frontline research tools due to the rapidity in which target binding scFv can be obtained. Simple modifications of the standard affinity selection and amplification protocol allow for multiple targets to be panned simultaneously.
J, Castillo, B, Goodson, J, Winter
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Journal of Agricultural and Food Chemistry, 2016
Citreoviridin (CIT), a small food-borne mycotoxin produced by Penicillium citreonigrum, is generally distributed in various cereal grains and farm crop products around the world and has caused cytotoxicity as an uncompetitive inhibitor of ATP hydrolysis.
Rongzhi Wang +5 more
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Citreoviridin (CIT), a small food-borne mycotoxin produced by Penicillium citreonigrum, is generally distributed in various cereal grains and farm crop products around the world and has caused cytotoxicity as an uncompetitive inhibitor of ATP hydrolysis.
Rongzhi Wang +5 more
semanticscholar +1 more source
2001
Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
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Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
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Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library
2015B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ...
Dangaj D, Scholler N
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[Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2006To construct a human phage displayed single chain Fv antibody (scFv) library and screen specific scFv against rabies virus.Using a phage-display technique, IgVH and IgVL genes were cloned from peripheral blood lymphocytes from three donors immunized with the WISTAR PM strain vaccine.
Xiao-ling, Zhao +4 more
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High-Level Periplasmic Expression and Purificationof scFvs
2003The isolation of recombinant antibodies by phage display naturally leads to experiments to evaluate their biological and immunological properties. Although crude preparations may have their value in initial studies, the need often exists for highly purified protein that can be tested in vivo.
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