Results 191 to 200 of about 35,719 (245)
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Screening for scFv-fragments that are stable and active in the cytosol
Human Antibodies, 2020Intrabodies are antibodies that are not secreted but bind to their antigens inside the cell producing them. Intrabodies targeting antigens in the endoplasmatic reticulum were successfully used in vitro and in vivo. However, many target antigens interesting for research or therapy are located in the reducing environment of the cytosol, where correct ...
Stefanie, Behring +4 more
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Journal of Immunological Methods, 2001
M13 scFv display libraries are frontline research tools due to the rapidity in which target binding scFv can be obtained. Simple modifications of the standard affinity selection and amplification protocol allow for multiple targets to be panned simultaneously.
J, Castillo, B, Goodson, J, Winter
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M13 scFv display libraries are frontline research tools due to the rapidity in which target binding scFv can be obtained. Simple modifications of the standard affinity selection and amplification protocol allow for multiple targets to be panned simultaneously.
J, Castillo, B, Goodson, J, Winter
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ScFv-CH3 and scFv-Fc Fusion Proteins
2001Earlier chapters (Chapters 2–16) have presented methods for producing small engineered antibodies (notably, scFv) from hybridomas or through selection from combinatorial libraries. It is now possible to generate fragments with a vast spectrum of specificities, including against targets previously inaccessible through hybridoma technology.
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High avidity scFv multimers; diabodies and triabodies
Journal of Immunological Methods, 1999Multivalent recombinant antibody fragments provide high binding avidity and unique specificity to a wide range of target antigens and haptens. This review describes how careful choice of linker length between V-domains creates new types of Fv modules with size, flexibility and valency suited to in vivo imaging and therapy. Further, we review the design
P J, Hudson, A A, Kortt
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Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library
2015B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ...
Dangaj D, Scholler N
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Efficient display of scFv antibodies on bacteriophage lambda
Journal of Immunological Methods, 2006In the present work we demonstrate the efficient display of functional scFv antibodies on the bacteriophage lambda capsid. A single-chain (scFv) anti-CEA antibody gene was cloned in two different vectors to obtain fusion of the scFv antibody to the N- or C-terminus of the bacteriophage lambda capsid protein D (gpD). Lambda bacteriophage assembly occurs
VACCARO P +5 more
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2001
Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
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Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
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Preparation and characterization of scFv for affinity purification of reteplase
Journal of Biochemical and Biophysical Methods, 2006The work was to explore the feasibility of protein affinity purification using ligand isolated from phage library. Reteplase was used as the model protein and a humanized semi-synthetic single chain fragment variable phage library as the source of ligand. After four rounds of biopanning, reteplase-specific phage clones were greatly enriched.
Zhigang, Guo +6 more
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Journal of Agricultural and Food Chemistry, 2016
Citreoviridin (CIT), a small food-borne mycotoxin produced by Penicillium citreonigrum, is generally distributed in various cereal grains and farm crop products around the world and has caused cytotoxicity as an uncompetitive inhibitor of ATP hydrolysis.
Rongzhi Wang +5 more
semanticscholar +1 more source
Citreoviridin (CIT), a small food-borne mycotoxin produced by Penicillium citreonigrum, is generally distributed in various cereal grains and farm crop products around the world and has caused cytotoxicity as an uncompetitive inhibitor of ATP hydrolysis.
Rongzhi Wang +5 more
semanticscholar +1 more source
[Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2006To construct a human phage displayed single chain Fv antibody (scFv) library and screen specific scFv against rabies virus.Using a phage-display technique, IgVH and IgVL genes were cloned from peripheral blood lymphocytes from three donors immunized with the WISTAR PM strain vaccine.
Xiao-ling, Zhao +4 more
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