Results 201 to 210 of about 35,719 (245)
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Selection of human anti-CD28 scFvs from a T-NHL related scFv library using ribosome display
Journal of Biotechnology, 2007Engineered antibodies have become an invaluable source of biopharmaceuticals against a wide range of diseases. About 200 antibody-based biologicals have been tested in clinical trials. Single chain variable fragments of antibodies (scFvs) provide binding specificity and offer an increased ease of in vitro display selection.
Achim, Rothe +7 more
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High-Level Periplasmic Expression and Purificationof scFvs
2003The isolation of recombinant antibodies by phage display naturally leads to experiments to evaluate their biological and immunological properties. Although crude preparations may have their value in initial studies, the need often exists for highly purified protein that can be tested in vivo.
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Journal of Immunological Methods, 1998
We describe here a method for the efficient and rapid analysis of antigen binding characteristics of recombinant antibodies (ab) selected by phage display. This novel approach combines the bacterial production of soluble single chain ab (scFv)-pIII fusion proteins on a microtiter scale with the detection of these fusion proteins via a pIII-specific ab.
M, Mersmann +9 more
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We describe here a method for the efficient and rapid analysis of antigen binding characteristics of recombinant antibodies (ab) selected by phage display. This novel approach combines the bacterial production of soluble single chain ab (scFv)-pIII fusion proteins on a microtiter scale with the detection of these fusion proteins via a pIII-specific ab.
M, Mersmann +9 more
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Production of Bispecific Antibodies: Diabodies and Tandem scFv
2012Recombinant bispecific antibodies have many different applications; especially promising is their therapeutic potential due to their ability to retarget an effector molecule or a cell to a disease-related target structure. In the last years, many formats have been developed: two commonly used are the bispecific diabody and the tandem scFv.
Nora, Hornig, Aline, Färber-Schwarz
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2021
Antibodies are used in many diagnostic, therapeutic, and research applications around the world. Traditionally, conventional full-length antibodies have been used for these applications, however, with the development of antibody engineering the use of antibody fragments has become more appealing.
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Antibodies are used in many diagnostic, therapeutic, and research applications around the world. Traditionally, conventional full-length antibodies have been used for these applications, however, with the development of antibody engineering the use of antibody fragments has become more appealing.
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2010
Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are
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Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are
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Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions
Nature Biotechnology, 2016S. Morita +10 more
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Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells
2003Michelle, de Graaf +3 more
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