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scFv Antibody: Principles and Clinical Application [PDF]
Clinical and Developmental Immunology, 2012 To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments.
Swee Yeap, Wan Yong Ho, Muhajir H Hamid
exaly +5 more sources
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Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions
Nature Biotechnology, 2016Kazuhiko Nakabayashi +2 more
exaly +2 more sources
OncoImmunology, 2016 We report here the successful vectorization of a hamster monoclonal IgG (namely J43) recognizing the murine Programmed cell death-1 (mPD-1) in Western Reserve (WR) oncolytic vaccinia virus.
Laurence Zitvogel
exaly +2 more sources
Reformatting of scFv Antibodies into the scFv-Fc Format and Their Downstream Purification
2014The scFv-Fc format allows for rapid characterization of candidate scFvs isolated from phage display libraries before conversion into a full-length IgG. This format offers several advantages over the phage display-derived scFv, including bivalent binding, longer half-life, and Fc-mediated effector functions.
Emil, Bujak +3 more
openaire +2 more sources
Journal of Immunological Methods, 2001
M13 scFv display libraries are frontline research tools due to the rapidity in which target binding scFv can be obtained. Simple modifications of the standard affinity selection and amplification protocol allow for multiple targets to be panned simultaneously.
J, Castillo, B, Goodson, J, Winter
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M13 scFv display libraries are frontline research tools due to the rapidity in which target binding scFv can be obtained. Simple modifications of the standard affinity selection and amplification protocol allow for multiple targets to be panned simultaneously.
J, Castillo, B, Goodson, J, Winter
openaire +2 more sources
Enzymatic Assembly for scFv Library Construction
2017Recombinant monoclonal antibodies can be established by displaying single-chain variable fragment (scFv) antibody libraries on phages and then biopanning against the target. For constructing superior scFv libraries, antibody light-chain variable region (VL) and heavy-chain variable region (VH) fragments must be assembled into scFvs without loss of ...
Mieko, Kato, Yoshiro, Hanyu
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Parallelized Microscale Expression of Soluble scFv
2019Antibody phage display is a key technology to generate recombinant, mainly human, antibodies for diagnostic and therapy, but also as tools for basic research. After antibody selection by "panning," a crucial step is the screening of monoclonal binders to isolate those which show antigen specificity.
Giulio, Russo +4 more
openaire +2 more sources
2001
Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
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Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
openaire +1 more source
Selection of human anti-CD28 scFvs from a T-NHL related scFv library using ribosome display
Journal of Biotechnology, 2007Engineered antibodies have become an invaluable source of biopharmaceuticals against a wide range of diseases. About 200 antibody-based biologicals have been tested in clinical trials. Single chain variable fragments of antibodies (scFvs) provide binding specificity and offer an increased ease of in vitro display selection.
Achim, Rothe +7 more
openaire +2 more sources