Results 111 to 120 of about 81,661 (282)

constclust: Consistent clusters for scRNA-seq

, 2019
Unsupervised clustering to identify distinct cell types is a crucial early step in analyses of scRNAseq data. Current methods are dependent on a number of parameters, whose effect on the resulting solution's accuracy and reproducibility are frequently uncharacterized – resulting in ad-hoc selection by users. constclust is a novel meta-clustering method
Virshup, Isaac, Jarny Choi, Bower, Neil, Hogan, Ben, Kim-Anh Le Cao, Wells, Christine   +5 more
openaire   +1 more source

Bufalin Inhibits the PI3K/AKT Pathway by Targeting GTF3C4 to Impede Breast Cancer Progression

Advanced Science, EarlyView.
Mechanisms of Bufalin‐Mediated Intervention in Breast Cancer. ABSTRACT Breast cancer incidence is rising globally, presenting challenges such as treatment side effects and drug resistance. Bufalin is a bufadienolides compound with potential anti‐cancer effects. This study shows that bufalin inhibits malignant proliferation of MDA‐MB‐231 and MCF‐7 cells
Siyu Guo, Xiaodong Chen, Haojia Wang, Jiying Zhou, Peiying Lu, Jiangying Liu, Keyan Chai, Jingyuan Zhang, Siyun Yang, Shan Lu, Yifei Gao, Zhengsen Jin, Xiaoyu Tao, Zhongdong Hu, Qinglin Li, Jiarui Wu   +15 more
wiley   +1 more source

PD‐1 Inhibits CD4+ TRM‐Mediated cDC1 Mobilization via Suppressing JAML in Human NSCLC

Advanced Science, EarlyView.
CD4+ tissue‐resident memory T cells (TRMs) in non‐small cell lung cancer recruit conventional type 1 dendritic cells via XCL1‐XCR1 signaling, orchestrating antitumor immunity. The costimulatory molecule JAML is essential for this process. PD‐1 blockade restores JAML expression and cDC1 mobilization, while JAML agonists synergize with anti‐PD‐1 therapy,
Zheyu Shao, Qinyuan Liu, Zhongwei Xin, Zhiyao Zhou, Mingjie Lin, Di Chen, Zhixing Hao, Yongyuan Chen, Wenxuan Wu, Shuyang Zhang, Xiaoke Chen, Xia Xu, Jinfan Li, Wei Lin, Yuhao Lu, Dang Wu, Pin Wu   +16 more
wiley   +1 more source

Clinical application of single‐cell RNA sequencing in disease and therapy

Clinical and Translational Medicine
Background The emergence of single‐cell RNA sequencing (scRNA‐seq) technology has revolutionized our capacity to study cell functions in complex tissue microenvironments. Traditional transcriptomic approaches, such as microarrays and bulk RNA sequencing,
Aisha Shigna Nadukkandy, Sowmiya Kalaiselvan, Lin Lin, Yonglun Luo   +3 more
doaj   +1 more source

Single-Cell RNA Sequencing of hESC-Derived 3D Retinal Organoids Reveals Novel Genes Regulating RPC Commitment in Early Human Retinogenesis. [PDF]

, 2019
The development of the mammalian retina is a complicated process involving the generation of distinct types of neurons from retinal progenitor cells (RPCs) in a spatiotemporal-specific manner.
An, Qin, Fan, Guoping, Hu, Youjin, Liu, Qinghuai, Mao, Xiying, Wang, Jinmei, Xi, Huiyu, Yang, Xian-Jie, Yuan, Songtao, Zhang, Xiangmei   +9 more
core  

Tendon Organoids Enable Functional Tendon Rejuvenation Through ALKBH5‐Dependent RNA Demethylation

Advanced Science, EarlyView.
FT organoids reverse the aged phenotype of tendon cells, reinstating a fetal‐like state. This breakthrough establishes a potent cell source for tendon tissue engineering, effectively advancing regenerative medicine. ABSTRACT Adult tendon injuries pose a major clinical challenge due to limited self‐repair capacity, resulting in suboptimal regeneration ...
Tian Qin, Aini Pan, Zhuoning Miao, Yanyan Zhao, Xianan Mo, Heng Sun, Chunmei Fan, Junyu Guo, Bingbing Wu, Weiliang Shen, Qiangqiang Zheng, Jun Lu, Xi Jiang, Zi Yin, Xiao Chen   +14 more
wiley   +1 more source

Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. [PDF]

, 2019
Tissue fibrosis is a major cause of mortality that results from the deposition of matrix proteins by an activated mesenchyme. Macrophages accumulate in fibrosis, but the role of specific subgroups in supporting fibrogenesis has not been investigated in ...
Abate, Adam R, Aran, Dvir, Bhattacharya, Mallar, Butte, Atul J, Chak, Suzanna, Fong, Valerie, Hsu, Austin, Liu, Leqian, Looney, Agnieszka P, Naikawadi, Ram P, Wolters, Paul J, Wu, Esther   +11 more
core  

Single‐Cell RNA Sequencing of Human Lung Tissues Reveals Metallothionein‐Positive T Cells as a Novel Potential Marker of Susceptibility to Chronic Obstructive Pulmonary Disease

Advanced Science, EarlyView.
Using single‐cell RNA sequencing of lung samples and flow cytometry of peripheral blood, we identified a novel T cell subset with high metallothionein (MT) expression. These MT‐high T cells suppress CD8+ T cell cytotoxicity and are progressively depleted from healthy controls to chronic obstructive pulmonary disease (COPD) patients, positioning them as
Zengqing Liu, Xiaoxia Ren, Mi Mu, Huanyu Long, Jialu Lv, Zhihong Feng, Jie Liu, Jiajia Wang, Hui Deng, Fanyu Shi, Muzhi Zhang, Ruoyang Zhang, Yang Liu, Mengyu Xu, Yang Fan, Zengtao Wang, Zhe Lv, Yan Chen, Chris J. Corrigan, Ying Sun, Yahong Chen, Chen Chen, Ye Cui, Wei Wang   +23 more
wiley   +1 more source

Immune Predictors of Radiotherapy Outcomes in Cervical Cancer

Advanced Science, EarlyView.
This study reveals dynamic immune remodeling in cervical cancer following radiotherapy. Single‐cell analysis identifies the C3/C3AR1 axis as a central mediator of epithelial–myeloid crosstalk, whose inhibition reduces treatment efficacy in mice. Guided by these insights, the eight‐feature machine‐learning model: Cervical Cancer Radiotherapy Immune ...
Linghao Wang, Jie Zhu, Zequn Ding, Zhiyuan Xie, Xingchen Liu, Feihong Zhang, Xiaojun Liu, Yan Zhang, Haiyan Chen   +8 more
wiley   +1 more source

Machine Learning for Integrating Data in Biology and Medicine: Principles, Practice, and Opportunities

, 2018
New technologies have enabled the investigation of biology and human health at an unprecedented scale and in multiple dimensions. These dimensions include a myriad of properties describing genome, epigenome, transcriptome, microbiome, phenotype, and ...
Goldenberg, Anna, Hoffman, Michael M., Leskovec, Jure, Nguyen, Francis, Wang, Bo, Zitnik, Marinka   +5 more
core   +1 more source