Results 11 to 20 of about 5,783 (206)

Structure of the substrate-engaged SecA-SecY protein translocation machine [PDF]

Nature Communications, 2019
Proteins are translocated across membranes through the Sec61/SecY channel. Here, the authors present the structure of a translocating peptide chain trapped inside the SecA-SecY complex which suggests how peptides are actively moved through the channel.
Chengying Ma, Xiaofei Wu, Dongjie Sun, Eunyong Park, Marco A. Catipovic, Tom A. Rapoport, Ning Gao, Long Li   +7 more
doaj   +2 more sources

Dynamic Organization of SecA and SecY Secretion Complexes in the B. subtilis Membrane. [PDF]

PLoS ONE, 2016
In prokaryotes, about one third of cellular proteins are translocated across the plasma membrane or inserted into it by concerted action of the cytoplasmic ATPase SecA and the universally conserved SecYEG heterotrimeric polypeptide-translocating pore ...
Alex Dajkovic, Elizabeth Hinde, Calum MacKichan, Rut Carballido-Lopez   +3 more
doaj   +2 more sources

Unlocking the Bacterial SecY Translocon [PDF]

Structure, 2016
The Sec translocon performs protein secretion and membrane protein insertion at the plasma membrane of bacteria and archaea (SecYEG/β), and the endoplasmic reticular membrane of eukaryotes (Sec61). Despite numerous structures of the complex, the mechanism underlying translocation of pre-proteins, driven by the ATPase SecA in bacteria, remains ...
Corey, Robin A, Allen, William J, Komar, Joanna, Masiulis, Simonas, Menzies, Sam, Robson, Alice, Collinson, Ian R   +6 more
openaire   +6 more sources

Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY [PDF]

The Journal of Cell Biology, 2005
During their biosynthesis, many proteins pass through the membrane via a hydrophilic channel formed by the heterotrimeric Sec61/SecY complex. Whether this channel forms at the interface of multiple copies of Sec61/SecY or is intrinsic to a monomeric complex, as suggested by the recently solved X-ray structure of the Methanococcus jannaschii SecY ...
Cannon, Kurt S., Or, Eran, Clemons, William M., Jr., Shibata, Yoko, Rapoport, Tom A.   +4 more
openaire   +7 more sources

Simulations of a Protein Translocation Pore:  SecY

Biochemistry, 2006
SecY is the central channel protein of the SecYEbeta translocon, the structure of which has been determined by X-ray diffraction. Extended (15 ns) MD simulations of the isolated SecY protein in a phospholipid bilayer have been performed to explore the relationship between protein flexibility and the mechanisms of channel gating.
Haider, S, Hall, B, Sansom, MS
openaire   +3 more sources

Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

, 2012
Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in ...
Foissac, Xavier, Danet, Jean-Luc, Malembic-Maher, Sylvie, Salar, Pascal, Safarova, Dana, Valova, Pavla, Navratil, Milan   +6 more
openaire   +4 more sources

Anomalous behavior of water inside the SecY translocon [PDF]

Proceedings of the National Academy of Sciences, 2015
Significance α-Helical membrane proteins are assembled cotranslationally into cell membranes with the aid of the heterotrimeric membrane-embedded translocon complex. The structures of several translocons reveal that the interior has an hourglass shape filled with water.
Capponi, Sara, Heyden, Matthias, Bondar, Ana-Nicoleta, Tobias, Douglas J, White, Stephen H   +4 more
openaire   +6 more sources

Structure of the SecY Complex Unlocked by a Preprotein Mimic

Cell Reports, 2012
The Sec complex forms the core of a conserved machinery coordinating the passage of proteins across or into biological membranes. The bacterial complex SecYEG interacts with the ATPase SecA or translating ribosomes to translocate secretory and membrane ...
Dilem Hizlan, Alice Robson, Sarah Whitehouse, Vicki A. Gold, Janet Vonck, Deryck Mills, Werner Kühlbrandt, Ian Collinson   +7 more
doaj   +5 more sources

Interrogating the Escherichia coli Epitranscriptome Via CRISPR Interference and Nanopore Native RNA Sequencing. [PDF]

Microbiologyopen
Epitranscriptomics is understudied in bacteria but has the ability to modulate antimicrobial resistance, virulence and pathogenicity. Pitt et al. implement two cutting‐edge techniques: CRISPR interference and Nanopore direct RNA sequencing along with functional assays to study RNA modifications in Escherichia coli.
Pitt ME, Zhang J, Nguyen ANT, Hall MB, Jebeli L, Featherstone LA, Myers GSA, Scott NE, Coin LJM.   +8 more
europepmc   +2 more sources

SecY-SecA fusion protein retains the ability to mediate protein transport. [PDF]

PLoS ONE, 2017
In bacteria, the membrane protein complex SecY/E/G and SecA ATPase are essential for protein translocation. About 30% of newly synthesized proteins in the cytosol are targeted to and translocated across the cytoplasmic membrane by the Sec factors ...
Yasunori Sugano, Arata Furukawa, Osamu Nureki, Yoshiki Tanaka, Tomoya Tsukazaki   +4 more
doaj   +1 more source