Results 101 to 110 of about 22,058 (247)

Aptamer Screening: Current Methods and Future Trend towards Non-SELEX Approach

Biosensors
Aptamers are nucleic acid sequences that specifically bind with target molecules and are vital to applications such as biosensing, drug development, disease diagnostics, etc.
Zhihui Fang, Xiaorui Feng, Fan Tang, Han Jiang, Shuyuan Han, Ran Tao, Chenze Lu   +6 more
doaj   +1 more source

Using Aptamers for Cancer Biomarker Discovery

Journal of Nucleic Acids, 2013
Aptamers are single-stranded synthetic DNA- or RNA-based oligonucleotides that fold into various shapes to bind to a specific target, which includes proteins, metals, and molecules.
Yun Min Chang, Michael J. Donovan, Weihong Tan   +2 more
doaj   +1 more source

Aptamers and SELEX in Chemistry & Biology

Chemistry & Biology, 2014
Nucleic acid aptamers, or simply aptamers, are oligonucleotides that bind specific ligands that vary from small molecules to proteins. An aptamer for a specific ligand is routinely identified through the process of systematic evolution of ligands by exponential enrichment, although some aptamers are found in nature as ligand-binding sites of special ...
Famulok, M., Mayer, G.
openaire   +3 more sources

Characterisation of aptamer–target interactions by branched selection and high-throughput sequencing of SELEX pools [PDF]

, 2015
Daniel M. Dupont, Niels Larsen, Jan K. Jensen, Peter A. Andreasen, Jørgen Kjems   +4 more
openalex   +1 more source

NMR monitoring of the SELEX process to confirm enrichment of structured RNA

Scientific Reports, 2017
RNA aptamers are RNA molecules that bind to a target molecule with high affinity and specificity using uniquely-folded tertiary structures. RNA aptamers are selected from an RNA pool typically comprising up to 1015 different sequences generated by ...
Ryo Amano, Kazuteru Aoki, Shin Miyakawa, Yoshikazu Nakamura, Tomoko Kozu, Gota Kawai, Taiichi Sakamoto   +6 more
doaj   +1 more source

Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity

Frontiers in Molecular Biosciences, 2018
Aptamers are structured nucleic acid molecules that can bind to their targets with high affinity and specificity. However, conventional SELEX (Systematic Evolution of Ligands by EXponential enrichment) methods may not necessarily produce aptamers of ...
Priya Kalra, Abhijeet Dhiman, Abhijeet Dhiman, William C. Cho, John G. Bruno, Tarun K. Sharma, Tarun K. Sharma   +6 more
doaj   +1 more source

DeepAptamer: Advancing high-affinity aptamer discovery with a hybrid deep learning model

Molecular Therapy: Nucleic Acids
Oligonucleotide aptamers are typically identified through a rigorous and time-consuming process known as systematic evolution of ligands by exponential enrichment (SELEX), which requires 20 to 30 iterative rounds to eliminate non/weak binding sequences ...
Xin Yang, Chi Ho Chan, Shanshan Yao, Hang Yin Chu, Minchuan Lyu, Ziqi Chen, Huan Xiao, Yuan Ma, Sifan Yu, Fangfei Li, Jin Liu, Luyao Wang, Zongkang Zhang, Bao-Ting Zhang, Lu Zhang, Aiping Lu, Yaofeng Wang, Ge Zhang, Yuanyuan Yu   +18 more
doaj   +1 more source

Selection of aptamers by cell-SELEX [PDF]

, 2014
Ge Zhang, Chao Liang, Baosheng Guo, Aiping Lü, Lingqiang Zhang, Ge Zhang   +5 more
openalex   +1 more source

Fabrication of graphene nanoplatelets embedded “partition cartridge” for efficient separation of target-bound ssDNA during SELEX

, 2021
Sagar Narlawar, Sonu Gandhi
openalex   +1 more source

The Study of a Hybrid-SELEX Method for the Development and Characterisation of an Aptamer to the Dendritic Cell Surface Receptor: Clec9A [PDF]

, 2022
Rebecca Palmer
openalex   +1 more source