Results 101 to 110 of about 20,710 (205)

DeepAptamer: Advancing high-affinity aptamer discovery with a hybrid deep learning model

Molecular Therapy: Nucleic Acids
Oligonucleotide aptamers are typically identified through a rigorous and time-consuming process known as systematic evolution of ligands by exponential enrichment (SELEX), which requires 20 to 30 iterative rounds to eliminate non/weak binding sequences ...
Xin Yang, Chi Ho Chan, Shanshan Yao, Hang Yin Chu, Minchuan Lyu, Ziqi Chen, Huan Xiao, Yuan Ma, Sifan Yu, Fangfei Li, Jin Liu, Luyao Wang, Zongkang Zhang, Bao-Ting Zhang, Lu Zhang, Aiping Lu, Yaofeng Wang, Ge Zhang, Yuanyuan Yu   +18 more
doaj   +1 more source

Plate cell – SELEX v2 [PDF]

, 2019
Aptamers are short single-stranded oligonucleotide (DNA or RNA) molecules with the ability to bind to other molecules with high affinity and specificity. Nowadays, aptamers are used as biosensing molecules with broader applications, such as ribozymes (RNA enzyme molecules with catalytic activities); riboswitches (modulating translational activities ...
openaire   +1 more source

Specific Aspects of SELEX Protocol: Different Approaches for ssDNA Generation

Methods and Protocols
Background: Synthetic DNA aptamers are a class of molecules with potential applications in medicine, serving as molecular sensors or ligands for targeted drug delivery. Systematic evolution of ligands by exponential enrichment (SELEX) is a technology for
Alexandr Garanin, Andrey Shalaev, Lidia Zabegina, Ekaterina Kadantseva, Tatiana Sharonova, Anastasia Malek   +5 more
doaj   +1 more source

SELEX Technologieentwicklung im Hochdurchsatz

, 2012
The SELEX process for the selection of functional nucleic acids (here: aptamers) is, although frequently modified, still a time and therefore cost- intensive process. A parallel and semiautomated selection of DNA aptamers in 10 rounds against Streptavidin was first conducted according to conventional protocols (cloning, Sanger sequencing), and then ...
openaire   +2 more sources

Sequence-based modeling of low-affinity transcription factor-DNA binding through deep learning. [PDF]

NAR Genom Bioinform
Wang Y, Li J, Chiu TP, Xin B, Rohs R.
europepmc   +1 more source

Development of an aptamer-based rapid diagnostic assay for <i>Salmonella</i> detection using whole-cell SELEX technique. [PDF]

Microbiol Spectr
Aga AM, Mulugeta D, Motuma A, Gebreegziabxier Weldemariam A, Woldesemayat AA, Wakitole B, Woldemariyam FT, Bashea C, Kelel M, Teferi Z, Hunderra GC, Abubeker R, Gebremicael G, Ali A, Leta GT, Berihun N, Tadesse S, Oljira S, Nigussie D, Gemeda MT.   +19 more
europepmc   +1 more source

Novel Homo-SELEX for selecting universal aptamer for cross-species kidney injury biomarker KIM-1 in muti-scenario situ imaging. [PDF]

J Nanobiotechnology
Yang H, Du Z, Zhu L, Guo H, Che X, Cao Q, Zhu Y, He X, Dong Y, Huang K, Xu W.   +10 more
europepmc   +1 more source

Selection of ssDNA aptamers targeting the serine protease SPSFQ of Acinetobacter baumannii and development of an electrochemical impedance spectroscopy-based ultrasensitive SPSFQ biosensor. [PDF]

Mikrochim Acta
Özyurt C, Afşar M, Ülker G, Man E, Evran S, Sezgintürk MK.   +5 more
europepmc   +1 more source

From Selection to Use: Aptamers as Targeting Reagents in Hematology. [PDF]

Biomedicines
Albert B, Ebanks F, Gharagozloo K, Hai X, Ngu R, Munting S, McKeague M.   +6 more
europepmc   +1 more source

RaptScore: a large language model-based algorithm for versatile aptamer evaluation. [PDF]

Nucleic Acids Res
Kimura-Yamazaki A, Adachi T, Nakamura S, Nakamura Y, Hamada M.   +4 more
europepmc   +1 more source