Results 101 to 110 of about 12,731 (219)
DeepAptamer: Advancing high-affinity aptamer discovery with a hybrid deep learning model
Molecular Therapy: Nucleic AcidsOligonucleotide aptamers are typically identified through a rigorous and time-consuming process known as systematic evolution of ligands by exponential enrichment (SELEX), which requires 20 to 30 iterative rounds to eliminate non/weak binding sequences ...
Xin Yang +18 more
doaj +1 more source
SELEX-technology development in high-troughput
, 2012 Der SELEX-Prozess zur Selektion funktioneller Nukleinsäuren (hier: Aptamere) ist, obgleich mittlerweile vielfach modifiziert, noch immer ein zeit- und damit kostenintensives Verfahren.
Schütze, Tatjana
core +1 more source
Quantitative Characterization of Partitioning Stringency in SELEX
Maintaining stringent conditions in SELEX (Systematic Evolution of Ligands by EXponential enrichment) is crucial for obtaining high-affinity aptamers; however, excessive stringency greatly increases the risk of SELEX failure.
Eden, Teclemichael +3 more
core +1 more source
, 2012 RU reached at the plateau of the association curve for all different channels of the Biacore CM5 chip with DNA isolated from various rounds of SELEX (4, 7, 10, 12 and 14) using channels coated with ß-conglutin, gliadin crude extract and simply blocked ...
Marketa Svobodova (170632) +4 more
core +1 more source
Recent Advances in the Optimization of Nucleic Acid Aptamers and Aptasensors
BiosensorsNucleic acid aptamers are single-stranded DNA or RNA molecules that can bind to a target with high specificity and affinity, as screened by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
Yuan Wang, Mengyan Nie
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In vivo SELEX of bone targeting aptamer in prostate cancer bone metastasis model
, 2018 Lingxiao Chen,1,* Wei He,1,* Huichuan Jiang,1,* Longxiang Wu,1 Wei Xiong,1 Bolun Li,1 Zhihua Zhou,2 Yuna Qian3–5 1Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; 2School of Chemistry and Chemical ...
Wu L +7 more
core
Selex results on and production
Nuclear Physics B - Proceedings Supplements, 2003 Abstract The SELEX experiment (E781) is a 3-stage magnetic spectrometer for a high statistics study of hadroproduction of charm baryons out to large χF using 650 GeV/c Σ−, π− and p beams. The main features of the spectrometer are: a high precision silicon vertex system; powerful particle identification provided by TRD and RICH; forward decay ...
openaire +2 more sources
Inelastic Electron-Pion Scattering at FNAL (SELEX) [PDF]
, 1999 We describe the analysis status of SELEX electron-pion inelastic {pi}e{yields}{pi}'e'{gamma} and {pi}e{yields}{pi}'e'{pi}{sup 0} reaction ...
M. Moinester, A. Ocherashvili and V. Steiner
core
Selection conditions in the SELEX process.
, 2015 BACE1: β-Site amyloid precursor protein cleaving enzyme 1aA blank control well was introduced for counter selection since the 4th round.Selection conditions in the SELEX process.
Huiyu Liang (531468) +9 more
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Specific Aspects of SELEX Protocol: Different Approaches for ssDNA Generation
Methods and ProtocolsBackground: Synthetic DNA aptamers are a class of molecules with potential applications in medicine, serving as molecular sensors or ligands for targeted drug delivery. Systematic evolution of ligands by exponential enrichment (SELEX) is a technology for
Alexandr Garanin +5 more
doaj +1 more source