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DNA aptamers selection for Staphylococcus aureus cells by SELEX and Cell-SELEX
Molecular Biology Reports, 2022Staphylococcus aureus is the most common bacteria found in skin, soft tissues, bone, and bone prostheses infections. The aim of this study was to select DNA aptamers for S. aureus to be applied in the diagnosis of bacteria.We used SELEX (Systematic Evolution of Ligands by EXponencial Enrichment) for peptidoglycan followed by cell-SELEX with S.
Mariane Izabella Abreu de Melo +3 more
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Functional SELEX and Biomedical Applications of Aptamers: Beyond Molecular Recognition.
Angewandte ChemieAptamers, developed through SELEX (systematic evolution of ligands by exponential enrichment), are generally short oligonucleotide molecules with remarkable specificity and binding affinity to diverse biological targets.
Minze He +9 more
semanticscholar +1 more source
In silico approach for Post-SELEX DNA aptamers: A mini-review.
Journal of Molecular Graphics and Modelling, 2021Aptamers are short oligonucleotides that possess high specificity and affinity against their target. Generated via Systematic Evolution of Ligands by Exponential Enrichment, (SELEX) in vitro, they were screened and enriched.
Nor Azlina Ahmad +3 more
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Improving Aptamer Affinity and Determining Sequence-Activity Relationships via Motif-SELEX.
Journal of the American Chemical SocietyThe affinity of nucleic acid aptamers isolated in vitro via Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is often limited because the entire potential sequence space cannot be screened.
Haixiang Yu +7 more
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A microfluidic SELEX prototype
Analytical and Bioanalytical Chemistry, 2005Aptamers are nucleic acid binding species capable of recognizing a wide variety of targets ranging from small organic molecules to supramolecular structures, including organisms. They are isolated from combinatorial libraries of synthetic nucleic acid by an iterative process referred to as SELEX (Systematic Evolution of Ligands by Exponential ...
Glen, Hybarger +4 more
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Analytica Chimica Acta
This review summarizes the stepwise strategy and key points for magnetic beads (MBs)-based aptamer selection which is suitable for isolating aptamers against small and large molecules via systematic evolution of ligands by exponential enrichment (SELEX).
Ioana Manea +5 more
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This review summarizes the stepwise strategy and key points for magnetic beads (MBs)-based aptamer selection which is suitable for isolating aptamers against small and large molecules via systematic evolution of ligands by exponential enrichment (SELEX).
Ioana Manea +5 more
semanticscholar +1 more source
Journal of Genetic Engineering and Biotechnology
Aptamers are single-stranded oligonucleotide sequences capable of binding to specific ligands with high affinity. In this manner, they are like antibodies but have advantages such as lower manufacturing costs, lower immunogenicity, fewer batch-to-batch ...
D. Chinchilla-Cárdenas +6 more
semanticscholar +1 more source
Aptamers are single-stranded oligonucleotide sequences capable of binding to specific ligands with high affinity. In this manner, they are like antibodies but have advantages such as lower manufacturing costs, lower immunogenicity, fewer batch-to-batch ...
D. Chinchilla-Cárdenas +6 more
semanticscholar +1 more source
Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches.
Trends in Biotechnology, 2021Aptamers are structural single-stranded oligonucleotides generated in vitro to bind to a specific target molecule. Aptamers' versatility can be enhanced with nucleic acid mimics (NAMs) during or after a selection process, also known as systematic ...
Ricardo Oliveira +5 more
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Post-SELEX optimization of aptamers
Analytical and Bioanalytical Chemistry, 2016Aptamers are functional single-stranded DNA or RNA oligonucleotides, selected in vitro by SELEX (Systematic Evolution of Ligands by Exponential Enrichment), which can fold into stable unique three-dimensional structures that bind their target ligands with high affinity and specificity.
Shunxiang, Gao +3 more
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Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging.
Analytical Chemistry, 2021Cancer has become one of the most common diseases with high mortality in humans. Early and accurate diagnosis of cancer is of great significance to enhance the survival rate of patients.
Lie Li +7 more
semanticscholar +1 more source