Results 81 to 90 of about 56,769 (258)

The advent of clinical self-amplifying RNA vaccines

Molecular Therapy
Self-amplifying RNA (saRNA) technology is an emerging platform for vaccine development, offering significant advantages over conventional mRNA vaccines. By enabling intracellular amplification of RNA, saRNA facilitates robust antigen expression at lower doses, thereby enhancing both immunogenicity and cost-effectiveness. This review examines the latest
Irafasha C. Casmil, Jongwoo Jin, Eun-Jeong Won, Cynthia Huang, Suiyang Liao, Hyunjoo Cha-Molstad, Anna K. Blakney   +6 more
openaire   +2 more sources

The Aging Blood: Cellular Origins, Circulating Drivers, and Therapeutic Potential

Aging and Cancer, EarlyView.
As a conduit linking all organs, the blood system both reflects and actively drives systemic aging. This review highlights how circulating pro‐aging and antiaging factors and age‐associated hematopoietic stem cell dysfunction contribute to immunosenescence and multi‐organ decline, positioning the hematopoietic system as a target for aging intervention.
Hanqing He, Jianwei Wang
wiley   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying RNA Vaccines

Vaccines, 2020
messenger RNA (mRNA)-based vaccines combine the positive attributes of both live-attenuated and subunit vaccines. In order for these to be applied for clinical use, they require to be formulated with delivery systems.
Giulia Anderluzzi, Gustavo Lou, Simona Gallorini, Michela Brazzoli, Russell Johnson, Derek T. O’Hagan, Barbara C. Baudner, Yvonne Perrie   +7 more
doaj   +1 more source

Molecular mechanisms of neurogenic aging in the adult mouse subventricular zone [PDF]

, 2019
In the adult rodent brain, the continuous production of new neurons by neural stem/progenitor cells (NSPCs) residing in specialized neurogenic niches and their subsequent integration into pre-existing cerebral circuitries supports odour discrimination ...
Biagioni, Stefano, Cacci, Emanuele, Gioia, Roberta, Lupo, Giuseppe, Nisi, PAOLA SERENA   +4 more
core   +1 more source

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

Research progress on the quality control of mRNA vaccines

Expert Review of Vaccines
Introduction The mRNA vaccine technologies have progressed rapidly in recent years. The COVID-19 pandemic has accelerated the application of mRNA vaccines, with research and development and clinical trials underway for many vaccines.
Chaoying Hu, Yu Bai, Jianyang Liu, Yiping Wang, Qian He, Xuanxuan Zhang, Feiran Cheng, Miao Xu, Qunying Mao, Zhenglun Liang   +9 more
doaj   +1 more source

HSP90 inhibitors stimulate DNAJB4 protein expression through a mechanism involving N6-methyladenosine. [PDF]

, 2019
Small-molecule inhibitors for the 90-kDa heat shock protein (HSP90) have been extensively exploited in preclinical studies for the therapeutic interventions of human diseases accompanied with proteotoxic stress.
Chen, Xuemei, Dai, Xiaoxia, Li, Lin, Miao, Weili, Wang, Yinsheng, Zhao, Yonghui   +5 more
core   +1 more source

The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration. [PDF]

, 2019
Metabolic reprogramming is an active regulator of stem cell fate choices, and successful stem cell differentiation in different compartments requires the induction of oxidative phosphorylation.
Cunningham, Thomas J, Dall'Agnese, Alessandra, Duester, Gregg, Etxaniz, Usue, Latella, Lucia, Nicoletti, Chiara, Puri, Pier Lorenzo, Sacco, Alessandra, Sala, David, Stec, Michael J   +9 more
core   +3 more sources

Mannosylation of LNP Results in Improved Potency for Self-Amplifying RNA (SAM) Vaccines [PDF]

ACS Infectious Diseases, 2019
Mannosylation of Lipid Nanoparticles (LNP) can potentially enhance uptake by Antigen Presenting Cells, which are highly abundant in dermal tissues, to improve the potency of Self Amplifying mRNA (SAM) vaccines in comparison to the established unmodified LNP delivery system.
Roshan Goswami, Despo Chatzikleanthous, Gustavo Lou, Fabiola Giusti, Alessandra Bonci, Marianna Taccone, Michela Brazzoli, Simona Gallorini, Ilaria Ferlenghi, Francesco Berti, Derek T. O’Hagan, Carlo Pergola, Barbara C. Baudner, Roberto Adamo   +13 more
openaire   +2 more sources