Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B
Results in Chemistry, 2023 Pyridoxal 5′-phosphate (PLP) is a ubiquitous and versatile cofactor utilized by numerous enzymes involved in amino acid biosynthetic pathways. Immobilized PLP is a valuable tool to isolate unknown PLP-dependent enzymes in nature or to perform in vitro ...
Kun-Hwa Lee +2 more
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On the utility of immobilized phenylarsine oxide in the study of redox sensitive cardiac proteins [PDF]
Scientific ReportsReactive protein cysteine thiols are critical to sensing and transducing oxidant signals, often by induction of disulfide bonds that alter their activity or interactions.
Asvi Arora Francois +5 more
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Molecules, 2021 In this work, the effect of different immobilization procedures on the properties of a lipase obtained from the extremophilic microorganism Serratia sp.
Mónica Ruiz +7 more
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ISOLATION AND PURIFICATION OF A KRINGLE 5 FROM HUMAN PLASMINOGEN USING AH-SEPHAROSE [PDF]
Biotechnologia Acta, 2014 Our aim was to develop a method for isolation of human plasminogen kringle 5 possessing functional activity. The proposed method includes the following steps: hydrolysis of plasminogen with elastase, separation of mini-plasminogen from kringle fragments ...
Kapustianenko L. G. +3 more
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RPEL proteins are the molecular targets for CCG-1423, an inhibitor of Rho signaling. [PDF]
PLoS ONE, 2014 Epithelial-msenchymal transition (EMT) is closely associated with cancer and tissue fibrosis. The nuclear accumulation of myocardin-related transcription factor A (MRTF-A/MAL/MKL1) plays a vital role in EMT.
Ken'ichiro Hayashi +4 more
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Iron(III)-chelating resins X. Iron detoxification of human plasma with iron(III)-chelating resins [PDF]
, 1993 Iron detoxification of human blood plasma was studied with resins containing desferrioxamine B (DFO) or 3-hydroxy-2-methyl-4(1H)-pyridinone (HMP) as iron(III)-chelating groups.
Bantjes, A., Does, L. van der, Feng, M.
core +6 more sources
Purification and characterization of highly stable aldo-keto reductase from camel (Camelus dromedarius) liver [PDF]
Archives of Biological Sciences, 2013 Aldo-keto reductase (AKR) was purified to homogeneity from camel liver by ion exchange on Q Sepharose, affinity chromatography on Blue-Sepharose and 2,5-ADP-Sepharose 4B.
Al-Harbi Ibrahim A. +2 more
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Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose [PDF]
Applied and Environmental Microbiology, 2002 ABSTRACT Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the ...
Patricia A, Shields, Samuel R, Farrah
openaire +2 more sources
Chemically-Modified Sepharose 6B Beads for Collection of Circulating Tumor Cells
Biomolecules, 2023 The isolation and quantitative characterization of circulating tumor cells (CTCs) are of great importance in cancer diagnosis and prognosis. However, isolating and detecting CTCs in whole blood presents a significant challenge due to the low numbers of ...
Haiyan Chen +4 more
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Chromatography of Hedysarum extracts on modified Sepharose CL sorbents
Фундаментальная и клиническая медицина, 2019 Aim. To study the chromatographic efficiency of modified Sepharose CL-4B sorbents for separation, purification, and preparation of major components from Hedysarum theinum Krasnob. and Hedysarum neglectum Ledeb.
Yu. S. Fedorova +4 more
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