Results 1 to 10 of about 149,683 (183)
Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose [PDF]
Applied and Environmental Microbiology, 2002 ABSTRACT Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the ...
Samuel R. Farrah, Patricia A. Shields
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Active Subunits of Transaldolase Bound to Sepharose [PDF]
European Journal of Biochemistry, 1973 Individual subunits of yeast transaldolase were studied by the method of matrix‐bound derivatives. Native dimeric transaldolase was bound to Sepharose activated with low amounts of cyanogen bromide to give matrix‐bound transaldolase in which the majority of molecules were bound covalently via only one subunit.
William W.-C. Chan +2 more
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Insulin-Sepharose and the Dynamics of Insulin Action [PDF]
Proceedings of the National Academy of Sciences, 1971 In terms of the rate of accumulation of α-aminoisobutyric acid, mammary epithelial cells from mature virgin mice respond rapidly to insulin covalently bound to sepharose particles, but do not respond initially to soluble insulin in vitro . The response to insulin-sepharose is prevented if the cells are first exposed
Oka, T, Topper, Y J
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The properties of subunits of avidin coupled to Sepharose [PDF]
Biochemical Journal, 1973 Avidin that had been coupled to Sepharose 4B activated with CNBr retained over 90% of its biotin-binding capacity. When low concentrations of CNBr were used about 75% of the protein could be removed from the Sepharose by washing with guanidinium chloride (6 m).
E. John Toms, N. Michael Green
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Affinity chromatography of creatine phosphokinase on organomercurial‐sepharose [PDF]
FEBS Letters, 1975 Creatine phosphokinase (CPK, EC 2.7.3.2) occurs mainly in muscle where it catalyses the reversible transfer of phosphate between ADP and creatine phosphate [ 1 ] . The enzyme has been isolated by combining alcohol and salt fractionation [ 1 ] followed by DEAE-column chromatography [2,3] .
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Coupling Antibody to Cyanogen Bromide-Activated Sepharose [PDF]
, 2014 Antibodies will be immobilized on a cyanogen bromide-activated Sepharose for subsequent use in pull-down assays or immunoaffinity purification.
Daniel J. Leahy, Jennifer M. Kavran
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Coupling of glycosaminoglycans to agarose beads (Sepharose 4B) [PDF]
Biochemical Journal, 1971 1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2.
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Biological Activity of Insulin-Sepharose? [PDF]
Science, 1973 Pedro Cuatrecasas +2 more
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Binding of Tubulin to Substituted Sepharose. [PDF]
Acta Chemica Scandinavica, 1976 Jan Lind +5 more
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On the utility of immobilized phenylarsine oxide in the study of redox sensitive cardiac proteins. [PDF]
Sci RepFrancois AA +5 more
europepmc +1 more source