Results 101 to 110 of about 240,931 (342)

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

Molecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng   +5 more
doaj   +1 more source

Expression in Escherichia coli of a cloned DNA sequence encoding the pre-S2 region of hepatitis B virus [PDF]

, 1985
A DNA sequence encoding the entire pre-S2 region (amino acids 120-174; serotype ayw) of human hepatitis B virus envelope protein has been inserted into the lacZ gene of the plasmid pSKS105 yielding a recombinant, pWS3.
Acs, George, Christman, Judith K., Kent, Stephen B. H., Neurath, A. Robert, Offensperger, Wolf, Price, Peter, Strick, Nathan, Wahl, Silke   +7 more
core   +1 more source

Aberrant SUMOylation Restricts the Targetable Cancer Immunopeptidome

Advanced Science, EarlyView.
Pharmacological SUMOylation inhibition (SUMOi) counteracts tumor immune evasion by unmasking an immunogenic HLA‐I peptide and neoepitope repertoire. By restoring HLA‐I ligand availability through increased antigen processing and presentation, enhanced proteasomal cleavage, and modulated TAP1 peptide affinity, SUMOi boosts tumor immunogenicity ...
Uta M. Demel, Anna Meurer, Badeel Kh. Q. Zaghla, Bilge Atay, Daniel Steiert, Luca V. Hummel, Konstandina Isaakidis, Chuanbing Zang, Michael Korenkov, Marlon Schielin, Schayan Yousefian, Shima Mecklenbräuker, Marieluise Kirchner, Simon Haas, Antonia Busse, Philipp Mertins, Stefan Müller, Matthias Wirth, Martin G. Klatt, Ulrich Keller   +19 more
wiley   +1 more source

Beyond Catalytic Therapy: Copper‐Paeonol Nanozymes Disrupt Fascin‐Mediated Actin Bundling to Suppress Tumor Growth and Metastasis

Advanced Science, EarlyView.
Copper‐paeonol nanozymes target tumor‐specific reactive oxygen species generation and disrupt fascin‐mediated actin bundling, effectively suppressing tumor growth and metastatic colonization. Abstract Fascin, an actin‐bundling protein universally upregulated in metastatic tumors, drives tumor migration and invasion by promoting filopodia and ...
Peiying Zhang, Huajun Li, Yisen Wang, Jie Xiang, Lei Fan, Shengzhe Zhang, Lizeng Gao, Hua Dai, Juqun Xi   +8 more
wiley   +1 more source

Activity and Process Stability of Purified Green Pepper (Capsicum annuum) Pectin Methylesterase [PDF]

, 2004
Pectin methylesterase (PME) from green bell peppers (Capsicum annuum) was extracted and purified by affinity chromatography on a CNBr-Sepharose-PMEI column. A single protein peak with pectin methylesterase activity was observed.
Alonso J., Alonso J., Anthon G. E., Castaldo D., Crelier S., Denès J.-M., Doesburg J., Eagerman B. A., Fachin D., Giovane A., Gross K. C., Heuskeshoven J., Indrawati, Indrawati, Jen J. J., Korner B., Laats M. M., Lee Y. S., Lin T. P., Ly-Nguyen B., Ly-Nguyen B., Markovič O., Pressey R., Puri A., Ramesh M. N., Rexová-Benková L., Sethu K. M. P., Seyderhelm I., Seymour T. A., Van den Broeck I., Van den Broeck I., Van den Broeck I., Versteeg C., Wicker L., Yemenicioǧlu A.   +34 more
core   +2 more sources

Structure‐Based Development of Ultra‐Broad‐Spectrum 3C‐Like Protease Inhibitors

Advanced Science, EarlyView.
This study provides an in‐depth analysis of the substrate binding pocket of 3CLpros across all coronavirus species using bioinformatics and structural insights, revealing the critical impact of S2/S4 subsite diversity on the broad‐spectrum activity of approved therapeutics.
Haixia Su, Tianqing Nie, Guofeng Chen, Muya Xiong, Yumin Zhang, Guoqing Wu, Mengyuan You, Hang Xie, Jian He, Yanchao Xiong, Hangchen Hu, Wenfeng Zhao, Minjun Li, Gengfu Xiao, Leike Zhang, Yechun Xu   +15 more
wiley   +1 more source

Purificación de la proteína banda 3 de eritrocitos humanos (AE 1) Purification of band 3 protein from human erythrocytes (AE 1)

Revista Cubana de Hematología, Inmunología y Hemoterapia, 2007
Se purifica la proteína banda 3 a partir de membranas de eritrocitos humanos con rapidez y eficiencia, mediante una combinación de cromatografía de intercambio iónico en DEAE-celulosa y de afinidad mediante el empleo de una columna pCMB-Sepharose.
Ada Amalia Arce Hernández, Rinaldo Villaescusa Blanco, Julio César Merlín Linares   +2 more
doaj  

Characterization of a corticosteroid 21-dehydroxylase from the intestinal anaerobic bacterium, Eubacterium lentum.

Journal of Lipid Research, 1980
An oxygen-sensitive corticosteroid 21-dehydroxylase has been characterized in cell extracts of Eubacterium lentum. The enzyme was highly specific for corticosteroids containing and alpha-ketol structure and required FMNH2 or reduced benzyl viologen for ...
S D Feighner, P B Hylemon
doaj   +1 more source

Identification and purification of an endogenous receptor for the lectin pallidin from Polysphondylium pallidum. [PDF]

, 1982
We report the identification and purification of an endogenous carbohydrate-containing receptor of pallidin, the cell surface lectin implicated in mediating cell-cell adhesion in the cellular slime mold Polysphondylium pallidum.
Drake, DK, Rosen, SD
core   +1 more source

Coupling of glycosaminoglycans to agarose beads (Sepharose 4B) [PDF]

Biochemical Journal, 1971
1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2.
openaire   +2 more sources