Results 31 to 40 of about 149,683 (183)

Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding

Biotechnology Progress, EarlyView.
Abstract The production of disulfide‐containing recombinant proteins often requires refolding of inclusion bodies before purification. A pre‐refolding purification step is crucial for effective refolding because impurities in the inclusion bodies interfere with refolding and subsequent purification.
Shuichiro Kimura, Wataru Yamamoto, Ai Miyamoto, Koreyoshi Imamura, Junichiro Futami   +4 more
wiley   +1 more source

pH and conductivity transients during elution of IgG from protein A columns

Biotechnology Progress, EarlyView.
Abstract The evolution of pH and conductivity waves during elution of IgG from protein A columns is studied for the resin MabSelect PrismA as well as other commercial resins using glycine and acetate buffers as eluents. The effects of buffer composition, IgG load, and residence time are explored.
Rainer Hahn, Lukas Berger, Jürgen Beck, Giorgio Carta   +3 more
wiley   +1 more source

Artificial Lipidation of Antifungal Proteins and Antifungal Behavior: A Case Study with Cholesterylation

ChemBioChem, EarlyView.
The antifungal behavior of artificial palmitoylated or cholesterylated chitinase domain is studied from biological, chemical, and physical properties, highlighting the importance of the chemical properties of the lipid moiety covalently attached to chitinase domain to obtain effective antifungal activity by artificial protein lipidation through a ...
Muhammad Safaat, Kazuki Uchida, Pugoh Santoso, Ryo Sato, Toki Taira, Rie Wakabayashi, Masahiro Goto, Noriho Kamiya   +7 more
wiley   +1 more source

Mining Thermophile Genomes for New PETases with Exceptional Thermostabilities Using Sequence Similarity Networks

ChemBioChem, EarlyView.
Targeted genome mining identified thermostable enzymes (PETases) that hydrolyze the commonly used plastic polymer polyethylene terephthalate (PET). Thermostable enzymes are identified from thermophilic microbial sources. Experimental results reveal enzymatic activity on authentic PET films, highlighting potential for industrial application.
Zhenyu Hu, Kody Klupt, David L. Zechel, Zongchao Jia, Graeme Howe   +4 more
wiley   +1 more source

Cutting‐Edge Aptasensing Approaches for Electrochemical Detection of Exosomes Utilizing MXenes

ChemElectroChem, EarlyView.
This work notably summarizes research on 2D MXenes/MXene's‐hybrid‐based nanoplatforms patterned with aptamers as an alternative to antibodies affording highly sensitive and robust biosensing of exosomes as well as their efficient isolation. The exosomes are highlighted as a valuable source of biomarkers and with the immobilization approaches of ...
Andrej Vikartovský, Natália Košútová, Lenka Lorencová, Peter Kasák, Tomáš Bertók, Ján Tkáč   +5 more
wiley   +1 more source

Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin

Cytoskeleton, EarlyView.
ABSTRACT Actin filaments are dynamic polymers whose length depends on regulated monomer association and dissociation at their ends. Actin barbed‐end dynamics are relatively better understood, primarily due to the approximately tenfold faster subunit on/off rates at barbed versus pointed ends.
Sawako Yamashiro, Shashank Shekhar, Stefanie M. Novak, Sudipta Biswas, Carol C. Gregorio, Velia M. Fowler   +5 more
wiley   +1 more source

Hybrid Polarizing Solids with Extended Pore Diameters for Dissolution Dynamic Nuclear Polarization

Chemistry–Methods, EarlyView.
Versatile and easily filtrable hyperpolarizing solids are introduced, used to generate pure hyperpolarized solutions by dissolution dynamic nuclear polarization (dDNP) for nuclear magnetic resonance spectroscopy (NMR), compatible with virtually any existing polarizer.
Ekaterina V. Pokochueva, Nghia H. Le, Sylvie Guibert, Chloé Gioiosa, Quentin Stern, James Tolchard, Charlotte Bocquelet, Olivier Cala, Matthieu Cavaillès, Laurent Veyre, Otto Mankinen, Ville‐Veikko Telkki, Chloé Thieuleux, Sami Jannin   +13 more
wiley   +1 more source

β‐Catenin localization in the ctenophore Mnemiopsis leidyi suggests an ancestral role in cell adhesion and nuclear function

Developmental Dynamics, EarlyView.
Abstract Background The emergence of multicellularity in animals marks a pivotal evolutionary event, which was likely enabled by molecular innovations in the way cells adhere and communicate with one another. β‐Catenin is significant to this transition due to its dual role as both a structural component in the cadherin–catenin complex and as a ...
Brian M. Walters, Lucas J. Guttieres, Mayline Goëb, Stanley J. Marjenberg, Mark Q. Martindale, Athula H. Wikramanayake   +5 more
wiley   +1 more source

STIP1 drives Metabolic Reprogramming in Esophageal Squamous Cell Carcinoma via AHCY‐LDHA Axis

Exploration, EarlyView.
A schematic model illustrating that LCA can inhibit ESCC growth by inhibiting the STIP1/AHCY/LDHA axis. In brief. Jin et al. demonstrated that targeting STIP1 can significantly inhibit the progression of esophageal squamous cell carcinoma. The high expression of STIP1 increased the interaction between AHCY and LDHA and then AHCY recruits PRMT3 to ...
Guoguo Jin, Yanming Song, Mingyang Yan, Shaobo Fang, Yang Shao, Kexin Zhao, Meng Liu, Qinxin Guo, Xinyang Jia, Chengjuan Zhang, Zhenwei Wang, Kangdong Liu, Xiang Li, Simin Zhao, Mee‐Hyun Lee, Zhiping Guo, Zigang Dong   +16 more
wiley   +1 more source

Self‐Emulsifying Biocatalysis Based on an Amphipathic Protein BslA‐Modified Esterase Functioning as a Fine‐Tuned System for Oil Hydrolysis

Food Bioengineering, EarlyView.
BslA is a promising tool to create an robust self‐emulsifying biocatalysis platform by reducing the droplet sizes of emulsion and improving its stability. It could guarantee the efficient hydrolysis of astaxanthin esters catalyzed by Est3‐14 without extra emusifier, increasing the yield of free astaxanthin to 184 μg·mL−1.
Xiaoxuan Yao, Kunpeng Gao, Hao Dong, Qian Sun, Jingyuan Liu, Yang Hu, Jianan Sun, Xiangzhao Mao   +7 more
wiley   +1 more source