Results 31 to 40 of about 240,931 (342)
Cyclic nucleotide specificity of the activator and catalytic sites of a cGMP-stimulated cGMP phosphodiesterase from Dictyostelium discoideum [PDF]
, 1985 The cellular slime mold Dictyostelium discoideum has an intracellular phosphodiesterase which specifically hydrolyzes cGMP. The enzyme is activated by low cGMP concentrations, and is involved in the reduction of chemoattractant-mediated elevations of ...
Baraniak, Janina, +8 more
core +2 more sources
Preparation of antibodies type IgY against Salmonella typhi lipopolysaccharide in chicken eggs
مجلة مركز بحوث التقنيات الاحيائية, 2012 ipopolysaccharide was extracted from local isolate Sallmonella typhi (previously isolated and characterized) by hot EDTA method, and the extract was partially purified by gel filtration chromatography on sepharose Cl-6B gel.
Mouruj A. Alaubydi +2 more
doaj +1 more source
Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification. [PDF]
PLoS ONE, 2018 As the proteomics field continues to expand, scientists are looking to integrate cross-disciplinary tools for studying protein structure, function, and interactions. Protein purification remains a key tool for many characterization studies.
Julia G Fraseur, Tamara L Kinzer-Ursem
doaj +1 more source
Journal of Microbiology, Immunology and Infection, 2020 Background/Purpose: HtsA (Streptococcus heme transporter A) is the lipoprotein component of the streptococcal heme ABC transporter (HtsABC). The aim of this study is to investigate whether the HtsA protein has immunoprotective effect against group A ...
Yingli Song +6 more
doaj +1 more source
Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]
, 2009 International audienceAs evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Barrass, J. David +6 more
core +2 more sources
MoleculesIn this study, the effect of various immobilization methods on the biochemical properties of phospholipase C (PLC) from Bacillus cereus obtained from the oily soil located in Sfax, Tunisia, was described. Different supports were checked: octyl sepharose,
Ines Abdelkader +3 more
doaj +1 more source
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio [PDF]
, 2015 Calsequestrin (Casq) is a high capacity, low affinity Ca2+-binding protein, critical for Ca2+-buffering in cardiac and skeletal muscle sarcoplasmic reticulum. All vertebrates have multiple genes encoding for different Casq isoforms.
Argenton, Francesco +6 more
core +1 more source
Lipoprotein lipase-like activity in the liver of mice with Sarcoma 180.
Journal of Lipid Research, 1984 The triglyceride lipase (TGL) activity of liver homogenates of mice with Sarcoma 180 was measured. The liver homogenate of normal or tumor-bearing mice was treated with 0.25% Triton X-100 and centrifuged at 100,000 g for 60 min, and the supernatant was ...
H Masuno +5 more
doaj +1 more source
Chemical Engineering Transactions, 2014 An isolation of extracellular lipases from the endemic fungal strain Geotrichum candidum-M2 was performed by vacuum evaporation, precipitation with cold acetone and vacuum drying.
I. Mladenoska
doaj +1 more source
Purification and Characterization of Porcine Angiotensin Converting Enzyme 2
Shipin gongye ke-ji, 2022 Angiotensin converting enzyme 2 (ACE2) is one of the key enzymes involved in blood pressure regulation. Conventional purification method for ACE2 was complicated and with low efficacy.
Weiwei LIU +6 more
doaj +1 more source