Results 51 to 60 of about 149,683 (183)

Stabilized Extracellular Vesicle Formulations for Inhalable Dry Powder Development

Small, EarlyView.
Extracellular vesicles (EVs) hold promise for chronic obstructive pulmonary disease (COPD) treatment but face challenges with storage and delivery. This study demonstrates the stabilization of lung fibroblast‐derived EVs using inulin, enabling freeze‐drying and spray‐drying.
Eva M. Jansen, Luke van der Koog, Robin A.B. Elferink, Karim Rafie, Anika Nagelkerke, Reinoud Gosens, Henderik W. Frijlink, Wouter L.J. Hinrichs   +7 more
wiley   +1 more source

Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract

Small, EarlyView.
Cell nuclei have been reconstituted in membrane‐less compartments using Xenopus egg extract, but not within lipid bilayer‐enclosed compartments. This study modifies the inverted emulsion method by adding chloroform to the lipid‐dispersed oil and extending lipid monolayer formation at the oil–egg extract interface, enabling the encapsulation of egg ...
Sho Takamori, Hisatoshi Mimura, Toshihisa Osaki, Tomo Kondo, Miyuki Shintomi, Keishi Shintomi, Miho Ohsugi, Shoji Takeuchi   +7 more
wiley   +1 more source

Imaging Ligand‐Receptor Interactions at Single‐Protein Resolution with DNA‐PAINT

Small Methods, EarlyView.
A labeling approach for small ligands in DNA‐PAINT super‐resolution microscopy preserving ligand functionality is demonstrated. This method enables the study of ligand‐receptor interactions at single‐protein resolution providing detailed insights into their spatial arrangement and interactions. Here, the interaction of the Epidermal Growth Factor (EGF)
Monique Honsa, Isabelle Pachmayr, Larissa Heinze, Levent Bas, Luciano A. Masullo, Jisoo Kwon, Ana Perovic, Brenda Schulman, Ralf Jungmann   +8 more
wiley   +1 more source

Manipulation of Wnt/β‐Catenin Signaling by Synthetic Frizzled Agonist and LRP Antagonist in Organoid Cultures and In Vivo

Small Methods, EarlyView.
Harnessing synthetic key receptor modulators, this study presents a breakthrough in Wnt/β‐catenin regulation. The FZD agonist RRP‐pbFn bypasses LRP5/6 to drive organoid expansion and tissue regeneration, while the LRP antagonist RRP‐Dkk1c eradicates tumors. These tools enable precise pathway control, advancing regenerative and cancer therapies.
Quanhui Dai, Jiawen Wang, Zihuan Lin, Danni Yu, Hui Yang, Jinsong Wei, Xiaoyu Li, Hao Hu, Chao Ni, Bing Zhao   +9 more
wiley   +1 more source

Investigating Impacts of Amino Acids on the Structural Stability of Anionic Biomembranes

ChemSystemsChem, Volume 7, Issue 2, March 2025.
Glycine and lysine interact with the anionic membrane composed of POPC and POPG (1 : 1). Lysine partitions into the membrane and induces structural alterations, while the membrane remains impermeable to it. In contrast, glycine causes no physicochemical damage and permeates through the membrane.
Arslan Siddique, Lauren A. Lowe, Soumya Kanti De, Daniel W. K. Loo, Anton P. Le Brun, Andrew R. J. Nelson, Anna Wang   +6 more
wiley   +1 more source

Evaluating the Therapeutic Potential of Superoxide Dismutase in a Rat Model of Wet Age‐Related Macular Degeneration

FASEB BioAdvances, EarlyView.
Oral Superoxide Dismutase Therapy Mitigates Choroidal Neovascularization in a Laser‐Induced Rat Model of Wet Age‐Related Macular Degeneration. Oral Administration of GF103, a Superoxide Dismutase From Bacillus amyloliquefaciens, Reduces Choroidal Neovascularization (CNV) in a Laser‐Induced CNV Rat Model.
Hyun Chul Jeong, Chang Sik Cho, Jeong Hyun Kim, Inik Chang, Jeong Hun Kim   +4 more
wiley   +1 more source

Changes of the lipid membrane structures caused by chain‐length‐dependent doxorubicin embedment in PEGylated liposomes

Journal of Applied Crystallography, EarlyView.
Chain‐length‐dependent membrane changes in PEGylated liposomes induced by doxorubicin loading are revealed using small‐ and wide‐angle X‐ray scattering and cryo‐EM.Poly(ethylene glycol)‐grafted (PEGylated) liposomes receive increasingly more attention due to their practical applications in delivering vaccines, nutrients and drug molecules such as ...
Jia-Jhen Kang, Zhih-Chen Huang, Li-Wen Tang, Chun-Jen Su, Hua-De Gao, Hsien-Ming Lee, U-Ser Jeng   +6 more
wiley   +1 more source

CAV1 Exacerbates Renal Tubular Epithelial Cell Senescence by Suppressing CaMKK2/AMPK‐Mediated Autophagy

Aging Cell, Volume 24, Issue 5, May 2025.
This study revealed that the expression of CAV1 is increased in the renal tissues of D‐gal‐induced aging mice and human renal proximal tubular epithelial cells (HK‐2). Using a GST pull‐down assay, it was demonstrated that CAV1 can directly bind to CaMKK2 and inhibit its activity, thereby suppressing the AMPK‐mediated autophagy process and exacerbating ...
Liya Sun, Lujun Xu, Tongyue Duan, Yiyun Xi, Zebin Deng, Shilu Luo, Chongbin Liu, Chen Yang, Huafeng Liu, Lin Sun   +9 more
wiley   +1 more source

Echinococcus granulosus antigen B regulates T‐cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia

British Journal of Haematology, EarlyView.
During the pathological process of immune thrombocytopenia, signal transducer and activator of transcription 3 (STAT3) is activated by JAKs, leading to the production of a large number of inflammatory cytokines and promoting the activation of Th1, Th2 and Th17 cells.
Yingbin Yue, Yunfei Zhang, Yongfeng Cheng, Hongjie Jiao, Mei Yan   +4 more
wiley   +1 more source

P2Y14 receptor activation of platelets induces Ca2+ mobilization and Rho‐GTPase‐dependent motility that requires an interaction with P2Y1 receptors

British Journal of Pharmacology, EarlyView.
Background and Purpose Platelet function during inflammation is dependent on activation by endogenous nucleotides acting on purinergic receptors. The P2Y14 receptor has been reported to be expressed on platelets and is involved in leukocyte recruitment during inflammation.
Md Monir Hossain, Dingxin Pan, Kate L. Arkless, Khondaker Miraz Rahman, Clive P. Page, Kalwant S. Authi, Simon C. Pitchford   +6 more
wiley   +1 more source