Results 61 to 70 of about 240,931 (342)

Subfractionation of human high density lipoproteins by heparin-Sepharose affinity chromatography.

Journal of Lipid Research, 1980
A reproducible and quantitative subfractionation of human high density lipoproteins (HDL) by heparin-Sepharose affinity chromatography has been developed. Two elution methods (A and B) were used to subfractionate HDL(2) (d 1.063-1.125 g/ml) or total HDL (
K H Weisgraber, R W Mahley
doaj   +1 more source

Herpes simplex virus ICP27 protein directly interacts with the nuclear pore complex through NUP62, inhibiting host nucleocytoplasmic transport pathways [PDF]

, 2012
The herpes simplex virus ICP27 protein is important for the expression and nuclear export of viral mRNAs. Although several binding sites have been mapped along the ICP27 sequence for various RNA and protein partners including the transport receptor TAP ...
Arnold, Aubert, Bachi, Bonifaci, Braun, Bryant, Chen, Chen, Copeland, Cullen, Darshan, Ellison, Ellison, Escudero-Paunetto, Faria, Fischer, Flach, Fontaine-Rodriguez, Fontoura, Fornerod, Fridell, Gerace, Gruffat, Grüter, Gustin, Gustin, Gustin, Han, Hardwicke, Hernandez, Hiriart, Hofemeister, Hutten, Johnson, Kambach, Koffa, Larralde, Lengyel, Lengyel, Li, Lilley, Long, Macaulay, Malik, Malik, McCarthy, Mears, Mears, Mears, Meyer, Michael, Nelson, Park, Pasdeloup, Pasquinelli, Pearson, Pellet, Petersen, Phelan, Piñol-Roma, Pollard, Pritchard, Rice, Rice, Rice, Rice, Rojas, Saavedra, Sandri-Goldin, Satterly, Schmitt, Sekulovich, Siomi, Smith, Soliman, Soliman, Souki, Strambio-De-Castillia, Strässer, Tsuji, Wagstaff, Wente, Zhi, Zhi   +83 more
core   +3 more sources

Properties ofHuman Hemoglobin Immobilized on Sepharose 4B [PDF]

European Journal of Biochemistry, 1978
This paper reports the properties of human hemoglobin covalently bound to Sepharose 4B both in ‘high‐affinity’ and ‘low‐affinity’ conformations.The results suggest that the coupling reaction is strongly affected by the conformational changes linked to oxygenation of the protein.
M R, Rossi Fanelli, G, Amiconi, E, Antonini   +2 more
openaire   +2 more sources

Membrane Vesicles from Lacticaseibacillus Casei BL23 Exhibit Antimicrobial Activity Against Escherichia coli and Immunostimulatory Effects on Human Peripheral Blood Mononuclear Cells

Advanced Healthcare Materials, EarlyView.
Membrane vesicles derived from the probiotic Lacticaseibacillus casei BL23 demonstrate antimicrobial properties against Escherichia coli and a potential biological effect in improving the overall survival of C. elegans infected with Pseudomonas aeruginosa. These vesicles stimulated immune responses in primary cells without causing toxicity. Our results
Cecilia L. D'Antoni, Leila Pourtalebi Jahromi, Lorenzo Sana, Ana Paula Domínguez Rubio, Maja Dorfner, Jennifer Munkert, Heike Danzer, Philipp Arnold, Mikhail Lebedev, Esther Zanin, Oscar E. Pérez, Gregor Fuhrmann   +11 more
wiley   +1 more source

Subfractionation of human very low density lipoproteins by heparin-Sepharose affinity chromatography

Journal of Lipid Research, 1983
Very low density lipoproteins obtained from normolipidemic subjects were fractionated into subclasses by means of affinity chromatography on a heparin-Sepharose column in the presence of MnCl2. The four subfractions eluted at 0.05, 0.12, 0.20, and 0.38 M
E Trezzi, C Calvi, P Roma, A L Catapano
doaj   +1 more source

Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain [PDF]

, 1983
A calcium and calmodulin-dependent protein kinase has been purified from rat brain. It was monitored during the purification by its ability to phosphorylate the synaptic vesicle-associated protein, synapsin I.
Bennett, Mark K., Erondu, Ngozi E., Kennedy, Mary B.   +2 more
core  

Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

Advanced Healthcare Materials, EarlyView.
Commercial vascular grafts are made from ePTFE, a highly hydrophobic, foreign material that fails at a high rate in small‐diameter applications. Plasma polymer nanoparticles (PPN) are a versatile material functionalisation tool, used here to present fibrillin‐1 fragment PF8 on the graft surface.
Bob S. L. Lee, Yuen Ting Lam, Alex H. P. Chan, Praveesuda L. Michael, Timothy C. Mitchell, Jui Chien Hung, Miguel Santos, Khoon S. Lim, Richard P. Tan, Steven G. Wise   +9 more
wiley   +1 more source

PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L. [PDF]

Journal of Sciences, Islamic Republic of Iran, 2002
Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-
doaj  

Strategies to Improve the Lipophilicity of Hydrophilic Macromolecular Drugs

Advanced Healthcare Materials, EarlyView.
Hydrophilic macromolecular drugs can be successfully lipidized by covalent attachment of lipids, by hydrophobic ion pairing with negatively or positively charged surfactants, and by dry or wet reverse micelle formation. Lipophilicity enhancement of hydrophilic macromolecules has several benefits including stability and bioavailability improvement ...
Sera Lindner, Stefan Keim, Soheil Haddadzadegan, Odile Fernandez Romero, Katrin Zöller, Gabriel Stern, Ilaria Cesi, Krum Kafedjiiski, Andreas Bernkop‐Schnürch   +8 more
wiley   +1 more source

Expression of linoleate isomerase gene from Lactobacillus reuteri PYR8 in Pichia pastoris

浙江大学学报. 农业与生命科学版, 2006
Linoleate isomerase gene was amplified by PCR from chromosome of Lactobacillus reuteri PYR8, then the gene was cloned into Pichia pastoris expression vector pPIC9K. The recombinant plasmid pPIC9K-LI was transformed into P.
ZHANG Yan-he, ZHANG Lan-wei, HU Sen, BU Zhi-gao   +3 more
doaj   +1 more source