Results 71 to 80 of about 149,683 (183)

WNT5a export onto extracellular vesicles studied at single‐molecule and single‐vesicle resolution

The FEBS Journal, EarlyView.
WNT signaling, which orchestrates tissue development and homeostasis, is mediated by hydrophobic WNT proteins traveling from secreting to receiving cells on various carriers. Here, fusion constructs of fluorescent proteins with WNT5a were developed and functionally validated.
Antonia Schubert, Ajaree Mongkolsittisilp, Andrei Kobitski, Matthias Schulz, Oksana Voloshanenko, Meike Schaffrinski, Nadine Winkler, Michelle Neßling, Karsten Richter, Dominique Kranz, Karin Nienhaus, Dirk Jäger, Lorenz Trümper, Judith Büntzel, Claudia Binder, Gerd Ulrich Nienhaus, Michael Boutros   +16 more
wiley   +1 more source

Protocol for affinity enrichment of polyclonal autoantibodies from human plasma. [PDF]

STAR Protoc
Aljnabi A, Hagadorn KA, Neumann LE, Peterson ME, Scott B, Miura K, Ongoiba A, Doumbo S, Doumtabe D, Li S, Kayentao K, Traore B, Bolland S, Crompton PD, Hopp CS.   +14 more
europepmc   +1 more source

Structural and functional insights into extreme thermal stability and activity of two GH12 domains of a multidomain glycosidase from a hyperthermophilic euryarchaeon

The FEBS Journal, EarlyView.
The multidomain glycosidase MDG from the hyperthermophilic euryarchaeon Thermococcus sp. contains five protein domains: three catalytic domains (GH5, GH12‐1, and GH12‐2) and two cellulose‐binding modules (CBM2). In this study, the GH12‐1 and GH12‐2 domains were individually purified and biochemically characterized.
Kseniya S. Zayulina, Evgenii N. Frolov, Christina Stracke, Alexandra A. Klyukina, Anna N. Khusnutdinova, Peter Stogios, Tatiana Skarina, Alexander F. Yakunin, Peter N. Golyshin, Bettina Siebers, Tatiana E. Shugaeva, Ilya V. Kublanov   +11 more
wiley   +1 more source

Sinomenine Inhibits TOPK To Ameliorate Psoriasiform Dermatitis. [PDF]

ACS Omega
Lu H, Zeng F, Gong H, Du J, Gao W, Xu J, Cai X, Yang Y, Xiao H.   +8 more
europepmc   +1 more source

NOTIZEN: Gewinnung von Cobalamin-Coenzym-Sepharose / Preparation of Cobalamin Coenzyme-sepharose

Zeitschrift für Naturforschung C, 1973
Margarethe Schlecht, Otto Muller
openaire   +2 more sources

DmdA‐independent lag phase shortening in Phaeobacter inhibens bacteria under stress conditions

The FEBS Journal, EarlyView.
The lag phase is crucial for resuming bacterial growth and can be shortened by fueling the methionine cycle with methyl groups from DMSP or other methylated compounds. While DmdA is the primary DMSP demethylase in Phaeobacter inhibens, stress activates additional enzymes like Bmt. This enzymatic flexibility allows bacteria to exit the lag phase rapidly,
Delia A. Narváez‐Barragán, Martin Sperfeld, Einat Segev   +2 more
wiley   +1 more source

Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coli. [PDF]

STAR Protoc
Holzner C, Böttinger K, Huber CG, Dall E, Brandstetter H.   +4 more
europepmc   +1 more source

TENT5C functions as a corepressor in the ligand‐bound glucocorticoid receptor and estrogen receptor α complexes

The FEBS Journal, EarlyView.
TENT5C is a suppressor of ERα and GR transcriptional activation. A specific LXXLL motif in TENT5C, absent in the other members of the TENT5 subfamily, mediates a stable interaction with the Ligand Binding Domain of ERα but not with that of GR.
Yin Li, Lalith Perera, Rebecca S. He, Marine Baptissart, Robert M. Petrovich, Marcos Morgan   +5 more
wiley   +1 more source

Development of Methods to Produce SARS CoV-2 Virus-Like Particles at Scale. [PDF]

Biotechnol Bioeng
Edeling MA, Earnest L, Carrera Montoya J, Yap AHY, Mumford J, Roberts J, Wong CY, Hans D, Grima J, Bisset N, Bodle J, Rockman S, Torresi J.   +12 more
europepmc   +1 more source

Specific binding of bovine immunoglobulins to Sepharose and Sephadex [PDF]

FEBS Letters, 1983
Yuji Inada, Teruaki Imada, Yuji Saito, Hiroshi Shimada   +3 more
openaire   +3 more sources