Results 41 to 50 of about 32,322,339 (203)

Expressed protein ligation for a large dimeric protein [PDF]

, 2011
Expressed protein ligation (EPL) is a protein engineering tool for post-translational ligation of protein or peptide fragments. This technique allows modification of specific parts of proteins, opening possibilities for incorporating probes for ...
NMR Spectroscopy, Karagöz, G.E., Sub Cellular Protein Chemistry, O. Hsieh, Sub NMR Spectroscopy, T. Sinnige, Rüdiger, S.G.D., Hsieh, O., Sinnige, T, G. E. Karagoz, S. G. D. Rudiger, Cellular Protein Chemistry   +11 more
core   +1 more source

Identification and functional analysis of Joka2, a tobacco member of the family of selective autophagy cargo receptors [PDF]

, 2011
Two main mechanisms of protein turnover exist in eukaryotic cells: the ubiquitin-proteasome system and the autophagylysosomal pathway. Autophagy is an emerging important constituent of many physiological and pathological processes, such as response to ...
Sirko, Agnieszka, Jolanta Łukomska, Surowiecki, Przemysław, Agnieszka Sirko, Lewandowska, Małgorzata, Liszewska, Frantz, Małgorzata Lewandowska, Anna Wawrzyńska, Grzegorz Moniuszko, Łukomska, Jolanta, Frantz Liszewska, Moniuszko, Grzegorz, Wawrzyńska, Anna, Gwozdecki, Rafał, Katarzyna Zientara-Rytter, Rafał Gwozdecki, Przemysław Surowiecki, Zientara-Rytter, Katarzyna   +17 more
core   +1 more source

Picky Hsp90-Every Game with Another Mate [PDF]

, 2017
In this issue of Molecular Cell, Sahasrabudhe et al. (2017) present a dramatically renovated functional cycle for the molecular chaperone Hsp90, which stimulates re-thinking of the mechanism of this vital protein folding ...
Sub Cellular Protein Chemistry, Rüdiger, Stefan G D, Radli, Martina, Cellular Protein Chemistry   +3 more
core   +2 more sources

The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence [version 2; peer review: 2 approved]

F1000Research
Sequestosome-1, encoded by the gene SQSTM1, functions as a bridge between ubiquitinated proteins and the proteasome or autophagosome, thereby regulating protein degradation pathways.
Peter S. McPherson, Carl Laflamme, Kathleen Southern, Riham Ayoubi, Walaa Alshafie, Irina Shlaifer   +5 more
doaj   +1 more source

Selective Autophagy Receptor p62/SQSTM1, a Pivotal Player in Stress and Aging

Frontiers in Cell and Developmental Biology, 2022
Efficient proteostasis is crucial for somatic maintenance, and its decline during aging leads to cellular dysfunction and disease. Selective autophagy is a form of autophagy mediated by receptors that target specific cargoes for degradation and is an ...
Anita V. Kumar, Joslyn Mills, Louis R. Lapierre   +2 more
doaj   +1 more source

UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling

eLife, 2021
To adapt in an ever-changing environment, cells must integrate physical and chemical signals and translate them into biological meaningful information through complex signaling pathways.
Stéphanie Torrino, Victor Tiroille, Bastien Dolfi, Maeva Dufies, Charlotte Hinault, Laurent Bonesso, Sonia Dagnino, Jennifer Uhler, Marie Irondelle, Anne-sophie Gay, Lucile Fleuriot, Delphine Debayle, Sandra Lacas-Gervais, Mireille Cormont, Thomas Bertero, Frederic Bost, Jerome Gilleron, Stephan Clavel   +17 more
doaj   +1 more source

Essential role for autophagy protein VMP1 in maintaining neuronal homeostasis and preventing axonal degeneration

Cell Death and Disease, 2021
Vacuole membrane protein 1 (VMP1), the endoplasmic reticulum (ER)-localized autophagy protein, plays a key role during the autophagy process in mammalian cells.
Panpan Wang, Xi Chen, Yuanyuan Wang, Congcong Jia, Xinyao Liu, Ying Wang, Haifeng Wu, Huaibin Cai, Han-Ming Shen, Weidong Le   +9 more
doaj   +1 more source

Computational Protein Design Force Field Optimization: A Negative Design Approach [PDF]

, 2007
An accurate force field is essential to computational protein design and protein folding studies. Proper force field tuning is problematic, however, due in part to the incomplete modeling of the unfolded state.
Alvizo, Oscar
core   +1 more source

Targeting Tex10 Overcomes Oxaliplatin Resistance by Competitively Disrupting the Non‐Canonical BAF Complex in Colorectal Cancer

Advanced Science, EarlyView.
This study reveals that Tex10 drives oxaliplatin resistance in colorectal cancer by competitively binding BRD9 to disrupt the ncBAF complex, thereby suppressing AMBRA1 transcription and ULK1‐mediated autophagy. Gemcitabine is identified as a direct Tex10 inhibitor that restores autophagy and overcomes resistance.
Ping Xu, Jie Huang, Xiaojin Gao, Lin Deng, Yanheng Deng, Denglu Wang, Chunlei Yu, Yunxiang Zhang, Jingdong Li, Xiaocong Xiang   +9 more
wiley   +1 more source

The ICP0 Protein of Herpes Simplex Virus 1 (HSV-1) Downregulates Major Autophagy Adaptor Proteins Sequestosome 1 and Optineurin during the Early Stages of HSV-1 Infection [PDF]

Journal of Virology, 2019
Autophagy is a homeostatic mechanism of cells to recycle components, as well as a defense mechanism to get rid of pathogens. Strategies that HSV-1 has developed to counteract autophagy have been described and involve inhibition of autophagosome formation or indirect mechanisms.
Hope, Waisner, Maria, Kalamvoki
openaire   +2 more sources