Results 41 to 50 of about 470,172 (244)

Directed ultrafast conformational changes accompany electron transfer in a photolyase as resolved by serial crystallography. [PDF]

Nat Chem
Charge-transfer reactions in proteins are important for life, such as in photolyases which repair DNA, but the role of structural dynamics remains unclear.
Cellini A, Shankar MK, Nimmrich A, Hunt LA, Monrroy L, Mutisya J, Furrer A, Beale EV, Carrillo M, Malla TN, Maj P, Vrhovac L, Dworkowski F, Cirelli C, Johnson PJM, Ozerov D, Stojković EA, Hammarström L, Bacellar C, Standfuss J, Maj M, Schmidt M, Weinert T, Ihalainen JA, Wahlgren WY, Westenhoff S.   +25 more
europepmc   +2 more sources

Robust image descriptor for machine learning based data reduction in serial crystallography. [PDF]

J Appl Crystallogr
This paper proposes a pipeline to categorize serial crystallography data, consisting of a real-time feature extraction algorithm, an image descriptor and a machine learning classifier.
Rahmani V, Nawaz S, Pennicard D, Graafsma H.   +3 more
europepmc   +2 more sources

In situ counter-diffusion crystallization and long-term crystal preservation in microfluidic fixed targets for serial crystallography. [PDF]

J Appl Crystallogr
User-friendly in situ counter-diffusion crystallization and long-term storage setups in microfluidic fixed targets provide a reliable approach to forming large crystals and maintaining their hydration for weeks, allowing ample time to grow, select and ...
Liu Z, Gu K, Shelby M, Roy D, Muniyappan S, Schmidt M, Narayanasamy SR, Coleman M, Frank M, Kuhl TL.   +9 more
europepmc   +2 more sources

Guide to serial synchrotron crystallography

Current Research in Structural Biology
Serial crystallography (SX) is an emerging technique that can be used to determine the noncryogenic crystal structure of macromolecules while minimizing radiation damage.
Ki Hyun Nam
doaj   +3 more sources

Application of Serial Crystallography for Merging Incomplete Macromolecular Crystallography Datasets

Crystals
In macromolecular crystallography (MX), a complete diffraction dataset is essential for determining the three-dimensional structure. However, collecting a complete experimental dataset using a single crystal is frequently unsuccessful due to poor crystal
Ki Hyun Nam
doaj   +2 more sources

Serial crystallography using synchrotron radiation [PDF]

IUCrJ, 2014
A brief history is given of how X-ray diffraction data from crystals have been recorded. Today there are new possibilities, spawned by the availability of free electron lasers that produce powerful femtosecond long X-ray pulses.
Michael G. Rossmann
doaj   +3 more sources

X-ray lasers and serial crystallography [PDF]

IUCrJ, 2015
A summary is given of the achievements and opportunities which resulted from the first use of an X-ray laser for serial crystallography and related methods in 2009.
John C. H. Spence
doaj   +4 more sources

Serial crystallography using automated drop dispensing [PDF]

Journal of Synchrotron Radiation, 2021
Automated, pulsed liquid-phase sample delivery has the potential to greatly improve the efficiency of both sample and photon use at pulsed X-ray facilities. In this work, an automated drop on demand (DOD) system that accelerates sample exchange for serial femtosecond crystallography (SFX) is demonstrated.
Zhen Su, Joshua Cantlon, Lacey Douthit, Max Wiedorn, Sébastien Boutet, Jan Kern, Chun Hong Yoon, Daniel DePonte   +7 more
openaire   +5 more sources

Nanoflow electrospinning serial femtosecond crystallography [PDF]

Acta Crystallographica Section D Biological Crystallography, 2012
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min−1to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min−1and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or ...
Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J.   +37 more
openaire   +2 more sources

Serial Electron Diffraction Data Processing With diffractem and CrystFEL

Frontiers in Molecular Biosciences, 2021
Serial electron diffraction (SerialED) is an emerging technique, which applies the snapshot data-collection mode of serial X-ray crystallography to three-dimensional electron diffraction (3D Electron Diffraction), forgoing the conventional rotation ...
Robert Bücker, Robert Bücker, Pascal Hogan-Lamarre, Pascal Hogan-Lamarre, Pascal Hogan-Lamarre, R. J. Dwayne Miller, R. J. Dwayne Miller   +6 more
doaj   +1 more source