Results 81 to 90 of about 7,535 (227)

Structure‐Based Development of Ultra‐Broad‐Spectrum 3C‐Like Protease Inhibitors

Advanced Science, EarlyView.
This study provides an in‐depth analysis of the substrate binding pocket of 3CLpros across all coronavirus species using bioinformatics and structural insights, revealing the critical impact of S2/S4 subsite diversity on the broad‐spectrum activity of approved therapeutics.
Haixia Su, Tianqing Nie, Guofeng Chen, Muya Xiong, Yumin Zhang, Guoqing Wu, Mengyuan You, Hang Xie, Jian He, Yanchao Xiong, Hangchen Hu, Wenfeng Zhao, Minjun Li, Gengfu Xiao, Leike Zhang, Yechun Xu   +15 more
wiley   +1 more source

Time-resolved structural studies with serial crystallography: A new light on retinal proteins

Structural Dynamics, 2015
Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy.
Valérie Panneels, Wenting Wu, Ching-Ju Tsai, Przemek Nogly, Jan Rheinberger, Kathrin Jaeger, Gregor Cicchetti, Cornelius Gati, Leonhard M. Kick, Leonardo Sala, Guido Capitani, Chris Milne, Celestino Padeste, Bill Pedrini, Xiao-Dan Li, Jörg Standfuss, Rafael Abela, Gebhard Schertler   +17 more
doaj   +1 more source

FELIX: an algorithm for indexing multiple crystallites in X-ray free-electron laser snapshot diffraction images [PDF]

, 2017
A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented.
Adams, Afonine, Agarwal, Barty, Boutet, Chapman, Cornelius Gati, DePonte, Gati, Gildea, Gildea, Ginn, Guo, Hart, Hattne, Henry N. Chapman, Hunter, Ivan G. Kazantsev, Kazantsev, Kazantsev, Kenneth R. Beyerlein, Morawiec, Morawiec, Nicolai Fog-Gade Nielsen, Oleksandr Yefanov, Paithankar, Park, Peter M. Larsen, Philipp, Powell, Powell, Sauter, Schmidt, Schmidt, Sharma, Sierra, Stellato, Søren Schmidt, Sørensen, Thomas A. White, Vaughan, Weierstall, White, White, White, Yefanov, Zaefferer, Zhang, Zhang   +48 more
core   +3 more sources

IgG‐Bridging–Seeded Synergistic Aggregation of SARS‐CoV‐2 Spikes Underlies Potent Neutralization by a Low‐Affinity Antibody

Advanced Science, EarlyView.
Low‐affinity antibodies are frequently disregarded in discovery pipelines. This work reports P5‐1C8, a Class 1 SARS‐CoV‐2 antibody with weak trimer binding (KD‐to‐IC50 > 3700‐fold) yet potent neutralization of Omicron JN.1. Structural, biophysical, functional, and coarse‐grained simulations collectively demonstrate that transient inter‐spike IgG ...
Niannian Lv, Peng Chen, Xiaobin Dai, Hu Xu, Ziheng Li, Zelin Shan, Jinqian Li, Fenglin Guo, Yuanfang Chen, Jiayi Li, Yiqian Huang, Guizhi Dong, Yifan Jiang, Liang Chen, Xuanyu Nan, Hanjun Zhao, Kang Zhang, Shilong Fan, Yuanchen Dong, Dongsheng Liu, Xinquan Wang, Deli Huang, Xiaojing Pan, Chunying Chen, Zhihua Liu, Li‐Tang Yan, Qi Zhang, Linqi Zhang, Yuliang Zhao, Yuhe Renee Yang   +29 more
wiley   +1 more source

Analysis of Multi-Hit Crystals in Serial Synchrotron Crystallography Experiments Using High-Viscosity Injectors

Crystals, 2021
Serial Synchrotron Crystallography (SSX) is rapidly emerging as a promising technique for collecting data for time-resolved structural studies or for performing room temperature micro-crystallography measurements using micro-focused beamlines.
Marjan Hadian-Jazi, Peter Berntsen, Hugh Marman, Brian Abbey, Connie Darmanin   +4 more
doaj   +1 more source

Choosing the optimal spectroscopic toolkit to understand protein function [PDF]

, 2017
Spectroscopy was one of the earliest methods used to study the properties and reactions of proteins, and remains one of the most powerful and widely used approaches to this day. A sometimes bewildering range of spectroscopies is now available, applicable
Andrew, Barth, Benfatto, Berera, Best, Billeter, Bui, Cheung, Dworkowski, D’Angelo, Franco, Glockner, Gossert, Hough, Kern, Kovermann, Kowalska, Kutta, Le Sueur, Levantino, Li, McGeehan, Michael A. Hough, Miles, Murakawa, Orville, Ritter, Robustelli, Roder, Rygula, Strange, Strange, Svistunenko, Teilum, von Stetten, Wallace, Wishart, Wuthrich, Yang, Yano, Zuiderweg   +40 more
core   +1 more source

Microscale Mapping of Fiber Strain and Damage in Composite Wrinkled Laminates Using Computed Tomography Assisted Wide‐Angle X‐Ray Scattering

Advanced Science, EarlyView.
This study combines full‐field tomography with diffraction mapping to quantify radial (ε002$\varepsilon _{002}$) and axial (ε100$\varepsilon _{100}$) lattice strain in wrinkled carbon‐fiber specimens for the first time. Radial microstrain gradients (−14.5 µεMPa$\varepsilon \mathrm{MPa}$−1) are found to signal damage‐prone zones ahead of failure, which ...
Hoang Minh Luong, James Trevarthen, Igor Dolbnya, Wadwan Singhapong, Nicholas Pilato, Richard Butler, Jiraphant Srisuriyachot, Alexander J. G. Lunt   +7 more
wiley   +1 more source

The current status and future prospects of the Synchrotron Radiation Protein Crystallography Core Facility at NSRRC: a focus on the TPS 05A, TPS 07A and TLS 15A1 beamlines

Journal of Synchrotron Radiation
The Synchrotron Radiation Protein Crystallography Core Facility (SPXF) at the National Synchrotron Radiation Research Center has been pivotal in advancing structural biology research in Taiwan.
Chung-Kuang Chou, Chien-Chang Tseng, Cheng-Hung Chiang, Yi-Hui Chen, Yi-Chun Liu, Chen-Ying Huang, Chun-Hsiung Chao, Chun-Hsiang Huang   +7 more
doaj   +1 more source

Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals [PDF]

, 2017
We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray ...
Adriano, Luigi, Aquila, Andrew, Awel, Salah, Bajt, Saša, Barty, Anton, Beyerlein, Kenneth R., Chapman, Henry N., Horke, Daniel A., Hunter, Mark S., James, Daniel, Kirian, Richard A., Knoska, Juraj, Küpper, Jochen, Mariani, Valerio, Morgan, Andrew, Oberthür, Dominik, Robinson, Joseph S., Rode, Andrei V., Roth, Nils, Roy-Chowdhury, Shatabdi, Tolstikova, Alexandra, Weierstall, Uwe, Wiedorn, Max O., Xavier, P. Lourdu, Yefanov, Oleksandr   +24 more
core   +4 more sources

Transmission of Radio‐Frequency Waves and Nuclear Magnetic Resonance in Lanthanum Superhydrides

Advanced Science, EarlyView.
1H NMR and radio‐frequency transmission measurements of lanthanum superhydrides at 165 GPa reveals a transition at 260–280 K by a strong suppression of the signal intensity, significant changes in NMR spectra, including a dramatic decrease of the relaxation rate 1/T1T, and pronounced shielding effect, which may have a superconducting nature.
Dmitrii V. Semenok, Florian Bärtl, Di Zhou, Toni Helm, Sven Luther, Joachim Wosnitza, Ivan A. Troyan, Viktor V. Struzhkin, Hannes Kühne   +8 more
wiley   +1 more source