Results 41 to 50 of about 149 (148)

Insights into Siglec‐7 Binding to Gangliosides: NMR Protein Assignment and the Impact of Ligand Flexibility

Advanced Science, EarlyView.
The molecular recognition of the sugar moiety of GD3 and Gb3 (globotriaosylceramide) derivatives by Siglec‐7 has been thoroughly studied offering insights for cancer diagnostics and therapies. A combination of structural biology techniques, nuclear magnetic resonance (NMR) spectroscopy, physicochemical analysis, and in silico methods suggested that ...
Cristina Di Carluccio, Luis Padilla‐Cortés, Marta Tiemblo‐Martìn, Giulia Roxana Gheorghita, Rosario Oliva, Linda Cerofolini, Alessandro Antonio Masi, Celeste Abreu, Hsin‐Kai Tseng, Antonio Molinaro, Pompea Del Vecchio, Ondřej Vaněk, Chun‐Cheng Lin, Roberta Marchetti, Marco Fragai, Alba Silipo   +15 more
wiley   +1 more source

Serine proteinase inhibitors in seeds of Cycas siamensis and other gymnosperms

Phytochemistry, 2008
Seeds of 32 species selected from two of the four major groups of gymnosperms, the ancient Cycadales and the economically important Coniferales, were analysed for inhibitors (I) of the serine proteinases trypsin (T), chymotrypsin (C), subtilisin (S) and elastase (E) using isoelectric focusing (IEF) combined with gelatin replicas.
Konarev, A. V., Lovegrove, A., Shewry, P. R.   +2 more
openaire   +3 more sources

Post‐Translational Modifications in Cilia and Ciliopathies

Advanced Science, EarlyView.
This review synthesizes current understanding of post‐translational modifications (PTMs) in ciliary proteins and emphasizes their roles in ciliary formation, homeostasis, and signaling. This review also discusses the implication of PTM dysregulation in ciliopathies and explores therapeutic strategies targeting PTM‐modifying enzymes.
Jie Ran, Jun Zhou
wiley   +1 more source

Nuclear‐Localized BCKDK Facilitates Homologous Recombination Repair to Support Breast Cancer Progression and Therapy Resistance

Advanced Science, EarlyView.
Under DNA damage, tumor cells rely on efficient DNA repair for survival and therapy resistance. This study has demonstrated that BCKDK localizes to breast cancer cell nuclei, where it binds to and phosphorylates RNF8, thereby blocking ubiquitin‐mediated degradation of RAD51 and enhancing HRR. A selective BCKDK inhibitor synergizes with clinical agents,
Haiying Liu, Jiaqian Feng, Tingting Pan, Pinggen Zhang, Ling Ye, Zetan Jiang, Zilong Zhou, Qiankun Mao, Jian Li, Xinyi Yang, Ping Gao, De Huang, Huafeng Zhang   +12 more
wiley   +1 more source

Dual Role of CRABP2 in Colorectal Cancer: Oncogenesis via Nuclear RB1 and Cytoplasmic AFG3L2/SLC25A39 Axis, While Limiting Liver Metastasis through Cytoplasmic AFG3L2/PINK1/Parkin‐Mediated Mitophagy

Advanced Science, EarlyView.
CRABP2 plays a dual role in colorectal cancer: it promotes proliferation and suppresses apoptosis through the nuclear CRABP2/RB1 axis and the cytoplasmic AFG3L2/SLC25A39 axis. Meanwhile, it inhibits colorectal liver metastasis via AFG3L2/PINK1/Parkin‐mediated mitophagy.
Chuanxin Tian, Sheng Yang, Chuan Zhang, Renzhong Zhu, Chen Chen, Xiaowei Wang, Dongsheng Zhang, Qingyang Sun, Hengjie Xu, Hongxu Nie, Yue Zhang, Dongjian Ji, Junwei Tang, Kangpeng Jin, Yueming Sun   +14 more
wiley   +1 more source

Sortilin‐Mediated Rapid, Precise and Sustained Degradation of Membrane Proteins via mRNA‐Encoded Lysosome‐Targeting Chimera

Advanced Science, EarlyView.
The study identified sortilin as a promising LTR, enabling targeted degradation of oncogenic proteins through an mRNA‐encoded MedTAC strategy. In a mouse model, MedTACPTK7 reduced PTK7 by up to 80%, extended survival, and showed excellent pharmacokinetics without toxicity, providing a scalable platform for targeted therapies.
Xin Chang, Xinyu Qiu, Xiaoning Tong, Shaoju Gan, Weicheng Yi, Sitao Xie, Xiangsheng Liu, Chao Zuo, Weihong Tan   +8 more
wiley   +1 more source

Proteomics‐Empowered Microfluidic‐SERS Immunoassay for Identifying and Detecting Biomarkers of Micropapillary Lung Adenocarcinoma

Advanced Science, EarlyView.
The presence of a micropapillary (MPP) component is critical for lung adenocarcinoma (LUAD) surgery, yet reliable blood biomarkers remain lacking. This study integrates proteomics for biomarker identification, a nanomixing‐enhanced surface‐enhanced Raman spectroscopy (SERS) platform for sensitive detection, and machine learning for accurate ...
Dechun Zhang, Kaiming Peng, Hui Xu, Yanping Chen, Jing Wang   +4 more
wiley   +1 more source

Dynamic Cap‐Mediated Substrate Access and Potent Inhibitor Design of Monkeypox Virus I7L Protease

Advanced Science, EarlyView.
The first crystal structure of MPXV I7L protease, revealing its unique dimeric form is resolved. The structures and MD simulations uncovered a dynamic cap region that regulates substrate access to the active site. The structural basis of substrate recognition and the catalytic mechanism are elucidated, which led to the development of a FRET‐based assay
Haixia Su, Guoqing Wu, Muya Xiong, Yuhang Wang, Junyuan Cao, Mengyuan You, Yingchun Xiang, Tianqing Nie, Minjun Li, Gengfu Xiao, Leike Zhang, Qiang Shao, Yechun Xu   +12 more
wiley   +1 more source

PP1A Modulates the Efficacy of Lenvatinib Plus ICIs Therapy by Inhibiting Ferroptosis in Hepatocellular Carcinoma

Advanced Science, EarlyView.
PP1A dephosphorylates Keap1 at the S104 site, stabilizing and promoting the nuclear translocation of Nrf2, which upregulates the expression of antioxidant genes and PD‐L1. This leads to suppressed ferroptosis and enhanced immune evasion, thereby improving the efficacy of TKI and ICI combination therapy.
Jitong Zhou, Meng Gao, Shikun Zhang, Wing‐Wa Guo, Wenzhi He, Minghe Zhang, Xi Chen, Cairang Dongzhi, Xiaomian Li, Yufeng Yuan, Weijie Ma   +10 more
wiley   +1 more source

Nephronectin (NPNT) is a Crucial Determinant of Idiopathic Pulmonary Fibrosis: Modulating Cellular Senescence via the ITGA3/YAP1 Signaling Axis

Advanced Science, EarlyView.
Through a comprehensive multi‐omics analysis, this study identifies a marked reduction in Nephronectin (NPNT) expression within fibrotic lung tissue. This reduction impairs the binding capability to the ITGA3 receptor, consequently causing YAP1 to persist in the cytoplasm, where it undergoes degradation.
Jiayu Guo, Yan Wang, Qiudi Liu, Zhaoyang Luo, Xiaomu Tian, Yuquan Wang, Yang Liu, Zhiwei Ning, Yingying Guo, Huiying Gao, Xinyue Wang, Jinglong Feng, Mengmeng Liu, Dina Saifullina, Yixin Zhang, Tengfei Pan, Yu Bian, Tao Ban, Tianyu Li, Yunyan Gu, Haihai Liang   +20 more
wiley   +1 more source