Results 41 to 50 of about 237,743 (266)

Defining short linear motif binding determinants by phage display-based deep mutational scanning. [PDF]

Protein Sci
AbstractDeep mutational scanning (DMS) has emerged as a powerful approach for evaluating the effects of mutations on binding or function. Here, we developed a DMS by phage display protocol to define the specificity determinants of short linear motifs (SLiMs) binding to peptide‐binding domains.
Benz C, Maassen L, Simonetti L, Mihalic F, Lindqvist R, Tsitsa I, Konstantinou A, Jemth P, Överby AK, Davey NE, Ivarsson Y.   +10 more
europepmc   +6 more sources

Ehrlichia SLiM Ligand Mimetic Activates Notch Signaling in Human Monocytes

mBio, 2022
Ehrlichia chaffeensis evades innate host defenses by reprogramming the mononuclear phagocyte through mechanisms that involve the exploitation of multiple evolutionarily conserved cellular signaling pathways, including Notch.
LaNisha L. Patterson, Thangam Sudha Velayutham, Caitlan D. Byerly, Duc Cuong Bui, Jignesh Patel, Veljko Veljkovic, Slobodan Paessler, Jere W. McBride   +7 more
doaj   +1 more source

Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH

eLife, 2022
The human proteome is replete with short linear motifs (SLiMs) of four to six residues that are critical for protein-protein interactions, yet the importance of the sequence surrounding such motifs is underexplored.
Theresa Hwang, Sara S Parker, Samantha M Hill, Robert A Grant, Meucci W Ilunga, Venkatesh Sivaraman, Ghassan Mouneimne, Amy E Keating   +7 more
doaj   +1 more source

Understanding protein multifunctionality: from short linear motifs to cellular functions [PDF]

Cellular and Molecular Life Sciences, 2019
Moonlighting proteins perform multiple unrelated functions without any change in polypeptide sequence. They can coordinate cellular activities, serving as switches between pathways and helping to respond to changes in the cellular environment. Therefore, regulation of the multiple protein activities, in space and time, is likely to be important for the
Zanzoni, Andreas, Ribeiro, Diogo, Brun, Christine   +2 more
openaire   +3 more sources

Ehrlichia effector SLiM-icry: Artifice of cellular subversion

Frontiers in Cellular and Infection Microbiology, 2023
As an obligately intracellular bacterial pathogen that selectively infects the mononuclear phagocyte, Ehrlichia chaffeensis has evolved sophisticated mechanisms to subvert innate immune defenses. While the bacterium accomplishes this through a variety of
Nicholas A. Pittner, Regina N. Solomon, Duc-Cuong Bui, Jere W. McBride, Jere W. McBride, Jere W. McBride, Jere W. McBride, Jere W. McBride   +7 more
doaj   +1 more source

Orchestrating serine/threonine phosphorylation and elucidating downstream effects by short linear motifs [PDF]

Biochemical Journal, 2022
Cellular function is based on protein–protein interactions. A large proportion of these interactions involves the binding of short linear motifs (SLiMs) by folded globular domains. These interactions are regulated by post-translational modifications, such as phosphorylation, that create and break motif binding sites or tune the affinity of the ...
Johanna Kliche, Ylva Ivarsson
openaire   +2 more sources

iLIR : a web resource for prediction of Atg8-family interacting proteins [PDF]

, 2014
Macroautophagy was initially considered to be a nonselective process for bulk breakdown of cytosolic material. However, recent evidence points toward a selective mode of autophagy mediated by the so-called selective autophagy receptors (SARs).
Johansen, Terje, Kalvari, Ioanna, Mulakkal, Nitha Charles, Nezis, I. P., Osgood, Richard, Promponas, Vasilis J., Tsompanis, Stelios   +6 more
core   +1 more source

МЕБИЈУСОВА ТРАКА: ВРЕМЕНСКА ПЕТЉА У РОМАНУ ДЕЈАНА АЛЕКСИЋА ЦИПЕЛА НА КРАЈУ СВЕТА [PDF]

International Journal of Slavic Studies, 2020
This article investigates the time loop motif in Dejan Aleksić’s novel, A Shoe at the End of the World. Following a brief overview of the most important uses of the time motif in children’s literature, and positioning this paper within Nikolajeva’s (i ...
Тијана Тропин
doaj   +1 more source

A short linear motif in BNIP3L (NIX) mediates mitochondrial clearance in reticulocytes. [PDF]

Autophagy, 2012
Elimination of defective mitochondria is essential for the health of long-lived, postmitotic cells. To gain insight into this process, we examined programmed mitochondrial clearance in reticulocytes. BNIP3L is a mitochondrial outer membrane protein that is required for clearance.
Zhang J, Loyd MR, Randall MS, Waddell MB, Kriwacki RW, Ney PA.   +5 more
europepmc   +4 more sources

The identification of short linear motif-mediated interfaces within the human interactome. [PDF]

Bioinformatics, 2012
Abstract Motivation: Eukaryotic proteins are highly modular, containing multiple interaction interfaces that mediate binding to a network of regulators and effectors. Recent advances in high-throughput proteomics have rapidly expanded the number of known protein–protein interactions (PPIs); however, the molecular basis for the majority ...
Weatheritt RJ, Luck K, Petsalaki E, Davey NE, Gibson TJ.   +4 more
europepmc   +4 more sources