Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans. [PDF]
Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals. For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of ...
Akihiko Kameyama +2 more
doaj +1 more source
Gel filtration of sialoglycoproteins [PDF]
The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin).
openaire +2 more sources
Isolation and Characteristics of Human Urinary Sialoglycoproteins [PDF]
Three sialoglycoproteins (S1, S2, and S3) have been isolated from normal human urine by ultrafiltration, zone electrophoresis, gel chromatography, and ion-exchange chromatography. The average yields per litre of urine were 0.39 mg (S1), 0.40 mg (S2), and 1.9 mg (S3).
I, Berggård +3 more
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Objectives: To assess the biochemical indicators of the connective tissue of rat periodontium (collagen, proteoglycans, glycoproteins) in local gum damage associated with lipopolysaccharide-induced systemic inflammatory response (SIR).
Yelinskaya A. N. +2 more
doaj +1 more source
Inhibitory effects of Trypanosoma cruzi sialoglycoproteins on CD4+ T cells are associated with increased susceptibility to infection. [PDF]
BackgroundThe Trypanosoma cruzi infection is associated with severe T cell unresponsiveness to antigens and mitogens characterized by decreased IL-2 synthesis. Trypanosoma cruzi mucin (Tc Muc) has been implicated in this phenomenom.
Marise Pinheiro Nunes +14 more
doaj +1 more source
Bacterial sialoglycosidases in Virulence and Pathogenesis
Human oral microbiome and dysbiotic infections have been recently evidently identified. One of the major reasons for such dysbiosis is impairment of the immune system.
Preethi Sudhakara +2 more
doaj +1 more source
Sialylation of cell surface glycans plays an essential role in cell–cell interaction and communication of cells with their microenvironment. Among the tools that have been developed for the study of sialylation in living cells, metabolic oligosaccharide ...
Jodie Scache +6 more
doaj +1 more source
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio [PDF]
Calsequestrin (Casq) is a high capacity, low affinity Ca2+-binding protein, critical for Ca2+-buffering in cardiac and skeletal muscle sarcoplasmic reticulum. All vertebrates have multiple genes encoding for different Casq isoforms.
Argenton, Francesco +6 more
core +1 more source
Sialic acid transporter NanT participates in Tannerella forsythia biofilm formation and survival on epithelial cells [PDF]
Tannerella forsythia is a periodontal pathogen implicated in periodontitis. This gram-negative pathogen depends on exogenous peptidoglycan amino sugar N-acetylmuramic acid (NAM) for growth.
Frey, A.M. +4 more
core +1 more source
Sialoglycosylation of RBC in visceral leishmaniasis leads to enhanced oxidative stress, calpain-induced fragmentation of spectrin and hemolysis. [PDF]
Visceral leishmaniasis (VL) caused by the intracellular parasite Leishmania donovani accounts for an estimated 12 million cases of human infection. It is almost always associated with anemia, which severely complicates the disease course.
Sajal Samanta +5 more
doaj +1 more source

