Results 91 to 100 of about 34,646 (246)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

インターロイキン2 ジョウホウ デンタツ キコウ [PDF]

, 2003
Extensive studies on the IL-2 and IL-2 receptor system have established fundamental principles of immune responses and signal transducing mechanisms of hematopoietic cell proliferation and survival.
Asao Hironobu
core   +1 more source

Phosphorylation of p130Cas initiates Rac activation and membrane ruffling [PDF]

, 2008
Background Non-receptor tyrosine kinases (NTKs) regulate physiological processes such as cell migration, differentiation, proliferation, and survival by interacting with and phosphorylating a large number of substrates simultaneously.
Alok Sharma, Bruce J Mayer, DA Lauffenburger, AJ Ridley, M Vicente-Manzanares, S Even-Ram, A Hall, PG Charest, YL Wang, RA Klinghoffer, D Ilic, AJ Koleske, H Honda, M Hagel, PY Chan, MD Schaller, MA Schwartz, LA Cary, BS Cobb, TR Polte, DD Schlaepfer, DD Schlaepfer, LA Cary, RL Klemke, RL Klemke, DA Cheresh, MD Welch, G Giannone, AJ Ridley, RL Klemke, SY Cho, SY Cho, AJ Ridley, H Miki, S Eden, MT Brown, SM Thomas, J Schlessinger, AB Reynolds, M Matsuda, R Sakai, R Sakai, M Auvinen, T Nakamoto, LA Petch, PJ Ruest, LA Cary, PM Fonseca, SM Bockholt, K Vuori, K Fujiwara, A Sharma, A Sharma, KM Arndt, K Machida, V Petit, SK Sastry, E Zamir, I Kaverina, MT Harte, SH Lo, V Benard, WE Allen, D Cox, AH Bouton, M Tamada, Y Sawada, H Honda, J Leng, CM Wells, R Pankov, GM Rivera, GM Rivera   +72 more
core   +2 more sources

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Mechanism and function of Vav1 localisation in TCR signalling [PDF]

, 2012
The antigen-specific binding of T cells to antigen presenting cells results in recruitment of signalling proteins to microclusters at the cell-cell interface known as the immunological synapse (IS).
Carter, T, Faroudi, M, Ksionda, O, Köchl, R, Rapley, J, Rittinger, K, Saveliev, A, Smith-Garvin, JE, Tybulewicz, VL, Wülfing, C   +9 more
core   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Stronger inflammatory/cytotoxic T cell response in women identified by microarray analysis [PDF]

, 2008
Women develop chronic inflammatory autoimmune diseases like lupus more often than men. The mechanisms causing the increased susceptibility are incompletely understood, although estrogen is believed to contribute.
Anura Hewagama, Bruce C. Richardson, Dipak Patel, Faith M. Strickland, Sushma Yarlagadda   +4 more
core   +1 more source