Results 91 to 100 of about 93,598 (304)

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.)

BMC Genomics, 2010
Background Cucumber, Cucumis sativus L. is an important vegetable crop worldwide. Until very recently, cucumber genetic and genomic resources, especially molecular markers, have been very limited, impeding progress of cucumber breeding efforts ...
Simon Philipp W, Yang Luming, Senalik Douglas A, Cavagnaro Pablo F, Harkins Timothy T, Kodira Chinnappa D, Huang Sanwen, Weng Yiqun   +7 more
doaj   +1 more source

Tandem repeat copy-number variation in protein-coding regions of human genes

, 2005
BACKGROUND: Tandem repeat variation in protein-coding regions will alter protein length and may introduce frameshifts. Tandem repeat variants are associated with variation in pathogenicity in bacteria and with human disease.
O'Dushlaine, Colm T., Edwards, Richard, Park, Stephen D., Richard J Edwards, Colm T O'Dushlaine, Denis C Shields, Shields, Denis C., Stephen D Park   +7 more
core   +1 more source

Patient therapy outcome modeling in cancer organoids is improved by cancer‐associated fibroblasts and organoid assembly convolution

Molecular Oncology, EarlyView.
Patient‐derived organoids (PDOs) from pancreatic, colorectal, and gastric cancers were used to evaluate standard and experimental therapies. Incorporating cancer‐associated fibroblasts (CAFs) into organoid cultures improved patient therapy outcome prediction.
Marcin Grochowski, Liudmyla Dolinchuk, Michał Jerzak, Albert Gandurski, Tomasz Grochowski, Weronika Wojtyś, Maciej Zadrożny, Wojciech Kaźmierczak, Małgorzata Lenarcik, Marta Matejak‐Górska, Radosław Samsel, Tomasz Olesiński, Dawid Walerych   +12 more
wiley   +1 more source

Identification of polymorphic simple sequence repeats in the genome of the zebrafish

Genomics, 1992
The zebrafish has drawn a great deal of attention as a developmental system because it offers the ability to combine excellent embryology and genetics. Here, we report that simple sequence repeats are abundant in the zebrafish genome and are highly polymorphic between two outbred lines, making them useful markers for the construction of a genetic map ...
D J, Goff, K, Galvin, H, Katz, M, Westerfield, E S, Lander, C J, Tabin   +5 more
openaire   +2 more sources

Data Mining for Simple Sequence Repeats in Oil Palm Expressed Sequence Tags

, 2009
Expressed Sequence Tags or ESTs are small pieces of DNA sequence that are generated by sequencing either one or both ends of an expressed gene. ESTs provide researchers with a quick and inexpensive route for discovering new genes, for obtaining data on ...
Vadivel Arunachalam, Aikkal Riju
core  

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Transferable analysis of pea EST-SSRs on faba bean and its application

浙江大学学报. 农业与生命科学版, 2011
In order to develop novel molecular markers for faba bean (Vicia faba L.), the transferability of pea (Pisum sativum L.) EST-SSR (expressed sequence tag-simple sequence repeats) markers and their application were studied in faba bean germplasms.
GONG Ya-ming, XU Sheng-chun, MAO Wei-hua, LI Ze-yun   +3 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Genome-wide identification of microsatellite markers from cultivated peanut (Arachis hypogaea L.)

BMC Genomics, 2019
Background Microsatellites, or simple sequence repeats (SSRs), represent important DNA variations that are widely distributed across the entire plant genome and can be used to develop SSR markers, which can then be used to conduct genetic analyses and ...
Qing Lu, Yanbin Hong, Shaoxiong Li, Hao Liu, Haifen Li, Jianan Zhang, Haofa Lan, Haiyan Liu, Xingyu Li, Shijie Wen, Guiyuan Zhou, Rajeev K. Varshney, Huifang Jiang, Xiaoping Chen, Xuanqiang Liang   +14 more
doaj   +1 more source